Nell Gwyn’s many after-lives : taming ‘the Protestant Whore’ in 21st century popular fiction

Ever since her supposed self-fashioning as ‘the Protestant Whore’ in the 1660s, Nell Gwyn has become a figure of fascination, revamped and reinterpreted in a multitude of ways along the years: from the black and white films of the 1930s, the story of this Restoration orange seller turned Royal concubine continues to excite the imagination of not just film makers, but of novelists, artists and even jam makers nowadays, as much as it inflamed Restoration audiences. The aim of this paper is to analyse the discourse that lays at the basis of three modern-day reconstructions of Nell Gwyn’s figure in an attempt at drawing a connection between celebrity, pop culture and historical fiction so as to explain the reimagining of this actress as an innocent strumpet, a scheming shrew, a dignified lady and all things in between; this paper takes ideas on celebrity and historical fiction as the theoretical basis upon which to build the criticism of these revampings of Nell Gwyn to better understand the survival of her figure three centuries after her death.peer-reviewe

River Bed Response to Channel Width Variation: Theory and Experiments (HES 49)

Illinois Water Resources Center (USGS Project 04 Contract 14-08-0004-G2017unpublishednot peer reviewe

U.S. SDG Data Revolution Roadmap

One year after adopting the SDGs, in an addendum to its Open Government National Action Plan, the U.S. Government committed to develop an SDG Data Revolution Roadmap that "charts the future course of efforts to fill data gaps and build capacity to use data for decision-making and innovation to advance sustainable development." The U.S. Government's SDG Data Revolution Roadmap will outline the government's commitments-to-action from 2017-2018. With a deadline of June 2017, it will be developed by the U.S. Government "through an open and inclusive process that engages the full range of citizen, non-governmental, and private sector stakeholders."This report represents the beginning of that engagement process. On December 14, 2016, the Center for Open Data Enterprise and the Global Partnership for Sustainable Development Data convened a Roundtable to develop recommended priorities for the U.S. Government's SDG Data Revolution Roadmap The Roundtable brought together more than 40 stakeholders from government, civil society, and the private sector with expertise in achieving and promoting sustainable development

RNA-binding protein immunoprecipitation as a tool to investigate plant miRNA processing interference by regulatory proteins of diverse origin

Background: Due to the nature of viral RNA genomes, RNA viruses depend on many RNA-binding proteins (RBP) of viral and host origin for replication, dissemination and evasion of host RNA degradation pathways. Some viruses interfere with the microRNA (miRNA) pathway to generate better fitness. The development of an adjusted, reliable and sensitive ribonucleoprotein immunoprecipitation (RIP) assay is needed to study the interaction between RBP of different origin (including viral origin) and miRNA precursors. The method could be further applied to transiently expressed heterologous proteins in different plant species. Results: Here we describe a modified RIP assay applied to nuclear epitope-tagged proteins of heterologous origin and transiently expressed in Nicotiana benthamiana. The assay includes a combination of optimized steps as well as the careful selection of control samples and rigorous data analysis. It has proven efficient to detect and quantify miRNA processing intermediates associated with regulatory proteins. Conclusions: The RIP method described here provides a reliable tool to study the interaction of RBPs, such as transiently expressed regulatory proteins with lowly represented host RNA, as is the case of miRNA precursors. This modified method was efficiently adjusted to recover nuclear proteins and reduce unspecific background. The purification scheme optimized here for GFP-tagged proteins can be applied to a wide array of RBPs. The subsequent application of next-generation sequencing technologies will permit to sequence and characterize all RNA species bound in vivo by a given RBP.Fil: Marmisollé, Facundo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentin