19 research outputs found
NyelĆcsĆ betegsĂ©gek etiolĂłgiĂĄjĂĄnak Ă©s kezelĂ©sĂ©nek nĂ©hĂĄny Ășj klinikai Ă©s kĂsĂ©rletes aspektusa
The esophagus is one of the most inscrutable organs of the gastrointestinal tract, hereby it challenges all physicians who specialize on it, including gastroenterologists, surgeons and also primary care physicians.
The complexity of the esophagus manifests itself on the colorful spectrum of complications that develop on the ground of gastroesophageal reflux disease (GERD). It is already unexplainable why chronic acid regurgitation provokes reflux esophagitis in some patients, while in others no endoscopic or histologic sign of inflammation can be detected, yet patients have severe complains. Development of diverse functional and structural esophageal disorders have been described in association with gastroesophageal reflux disease, but to date, it is unclear whether these secondary pathologies are simple consequences of GERD or is it possible that some of them are defensive reactions? Is there an expediency in their development or is it mere coincidence? Do the presumed adaptive esophageal changes influence the therapeutic strategy? Gastroesophageal reflux disease has become an endemic malady worldwide in the past decades. Understanding the longterm impacts of acid regurgitation on the esophagus would be imperative in order to better comprehend the behavior of this organ and to improve our knowledge about the possible complications of GERD
âA vilĂĄg egy vĂĄrosbanâ1 Liverpooli EKF tapasztalatok
Az elmĂșlt idĆszakban, tekintve, hogy 2010-ben egy magyarorszĂĄgi vĂĄros, PĂ©cs viselte az EurĂłpa KulturĂĄlis FĆvĂĄrosa (EKF) cĂmet, egyre több ĂrĂĄs lĂĄtott napvilĂĄgot hazai szakfolyĂłiratokban is, gĂłrcsĆ alĂĄ vĂ©ve a fent nevezett uniĂłs projektet, annak törtĂ©nelmĂ©t, nemzeti sajĂĄtossĂĄgait, tĂĄrsadalmasĂtĂĄsĂĄt, tĂ©nyleges nĂ©pszerƱsĂ©gĂ©t. A nagyközönsĂ©g a pĂ©csi projektet könyv formĂĄjĂĄban is megismerhette. Jelen tanulmĂĄny tĂ©mĂĄjĂĄt tekintve ebbe a sorba illeszkedik, hiszen egyet a sikeres EKF programok közĂŒl igyekszik bemutatni, jelesĂŒl a liverpoolit. A dolgozat keretei nem teszik lehetĆvĂ©, hogy teljes spektrumĂĄt bejĂĄrjuk az elemzĂ©snek, hiszen megannyi kĂ©rdĂ©s vĂĄr megvĂĄlaszolĂĄsra az integrĂĄciĂł eme lakossĂĄg körĂ©ben is egyre ismertebb kezdemĂ©nyezĂ©sĂ©vel kapcsolatban. Arra azonban vĂĄllalkozni merĂŒnk, hogy a többszintƱ kormĂĄnyzĂĄs hatĂ©kony megvalĂłsulĂĄsĂĄt Ă©s az EKF program sikerĂ©t összevessĂŒk az angliai vĂĄros pĂ©ldĂĄjĂĄn keresztĂŒl. ĂrĂĄsunk cĂ©lja Liverpool esetĂ©n keresztĂŒl felhĂvni a figyelmet a kultĂșrĂĄban rejlĆ lehetĆsĂ©gekre, amelyek felhasznĂĄlĂĄsĂĄval elĂ©rhetĆvĂ© vĂĄlik egy korĂĄbban komoly gazdasĂĄgi, tĂĄrsadalmi problĂ©mĂĄkkal kĂŒzdĆ vĂĄros ĂĄtalakĂtĂĄsa, Ă©s az EKF programnak köszönhetĆen nemzetközi szintƱ megismertetĂ©se. ĂllĂtĂĄsunk, hogy a többszintƱ kormĂĄnyzĂĄs megvalĂłsulĂĄsa (mindig a szereplĆk kĂvĂĄnt arĂĄnyĂș beavatkozĂĄsĂĄval), a döntĂ©shozatali mechanizmusok hierarchia központĂșsĂĄgĂĄnak mellĆzĂ©se, magĂĄban hordozza egy közössĂ©gi kezdemĂ©nyezĂ©s sikerĂ©t
A gastrooesophagealis refluxbetegsĂ©g nyelĆcsĆszövĆdmĂ©nyei: következmĂ©nyek vagy vĂ©dekezĆreakciĂłk? | Esophageal complications of gastroesophageal reflux disease: consequences or defensive reactions?
Absztrakt:
A gastrooesophagealis refluxbetegsĂ©g a felnĆtt lakossĂĄg több mint 10%-ĂĄt Ă©rinti.
A betegek nagy többsĂ©ge Ă©letmĂłdbeli vĂĄltoztatĂĄsokkal Ă©s hatĂĄsos savcsökkentĆ
szerekkel jĂłl kezelhetĆ. Az Ă©rintettek körĂŒlbelĂŒl 10%-a azonban a kezelĂ©s dacĂĄra
panaszos marad, Ă©s nĂĄluk sĂșlyos szövĆdmĂ©nyek fejlĆdhetnek ki. KĂŒlönös mĂłdon a
szövĆdmĂ©nyek egy rĂ©sze vĂ©dekezĆ jellegƱnek tƱnik, amivel a beteg panaszai
mĂ©rsĂ©klĆdhetnek, illetve mintha az ĂĄllapot sĂșlyosbodĂĄsĂĄnak Ă©s tovĂĄbbi
szövĆdmĂ©nyek kialakulĂĄsĂĄnak megelĆzĂ©sĂ©t cĂ©loznĂĄk. Az egyĂ©rtelmƱen
refluxszövĆdmĂ©nynek tekinthetĆ Barrett-oesophagusban jelentĆsen mĂ©rsĂ©klĆdhetnek
az Ă©letminĆsĂ©get rontĂł panaszok, mert a Barrett-hĂĄm sokkal ellenĂĄllĂłbb a
gyomorsav marĂł hatĂĄsĂĄval szemben, mint a nyelĆcsövet normĂĄlisan bĂ©lelĆ laphĂĄm. A
refluxszövĆdmĂ©nykĂ©nt kialakulĂł motilitĂĄszavarok (hipertenzĂv alsĂł
nyelĆcsĆsphincter, achalasia cardiae Ă©s cricopharyngealis achalasia) Ă©s
strukturĂĄlis elvĂĄltozĂĄsok (Schatzki-gyƱrƱ, nyelĆcsĆstrictura, subglotticus
tracheastenosis) pedig segĂtenek megelĆzni az aspirĂĄciĂłt, amely Ășjabb panaszok
megjelenĂ©sĂ©vel jĂĄrhat, Ă©s tovĂĄbbi sĂșlyos szövĆdmĂ©nyek kialakulĂĄsĂĄnak a
lehetĆsĂ©gĂ©t hordozza magĂĄban. Orv Hetil. 2017; 158(20): 763â769.
|
Abstract:
Gastroesophageal reflux disease affects more than 10% of the adult population.
Most patients can be effectively treated with lifestyle changes and adequate
acid-reducing therapy. However, about 10% of the patients remain symptomatic
despite treatment and severe complications may develop. Interestingly, some of
these complications seem to be a sort of defensive mechanism that may either
alleviate the patientâs symptoms or prevent developing further complications. In
Barrettâs esophagus, which can be unambigously considered as a complication of
gastroesophageal reflux disease, reflux symptoms ruining the quality of life may
significantly improve, since the metaplastic Barrett epithelium is much more
resistent to gastric acid, than the normal epithelial lining of the esophagus.
Furthermore, the motility disorders (hypertensive lower esophageal sphincter,
achalasia, cricopharyngeal achalasia) and structural changes (Schatzkiâs ring,
esophageal stricture, subglottic trachea stenosis), which develop as a
complication of reflux may help to prevent aspiration that can cause new
complaints and may lead to further complications. Orv Hetil. 2017; 158(20):
763â769
Light Control of Salt-Induced Proline Accumulation is Mediated by Elongated Hypocotyl 5 in Arabidopsis
Plants have to adapt their metabolism to constantly changing environmental conditions, among which the availability of light and water is crucial in determining growth and development. Proline accumulation is one of the sensitive metabolic responses to extreme conditions; it is triggered by salinity or drought and is regulated by light. Here we show that red and blue but not far-red light is essential for salt-induced proline accumulation, upregulation of Delta 1-PYRROLINE-5-CARBOXYLATE SYNTHASE 1 (P5CS1) and downregulation of PROLINE DEHYDROGENASE 1 (PDH1) genes, which control proline biosynthetic and catabolic pathways, respectively. Chromatin immunoprecipitation and electrophoretic mobility shift assays demonstrated that the transcription factor ELONGATED HYPOCOTYL 5 (HY5) binds to G-box and C-box elements of P5CS1 and a C-box motif of PDH1. Salt-induced proline accumulation and P5CS1 expression were reduced in the hy5hyh double mutant, suggesting that HY5 promotes proline biosynthesis through connecting light and stress signals. Our results improve our understanding on interactions between stress and light signals, confirming HY5 as a key regulator in proline metabolism
Light Control of Salt-Induced Proline Accumulation Is Mediated by ELONGATED HYPOCOTYL 5 in Arabidopsis
Plants have to adapt their metabolism to constantly changing environmental conditions, among which the availability of light and water is crucial in determining growth and development. Proline accumulation is one of the sensitive metabolic responses to extreme conditions; it is triggered by salinity or drought and is regulated by light. Here we show that red and blue but not far-red light is essential for salt-induced proline accumulation, upregulation of Delta 1-PYRROLINE-5-CARBOXYLATE SYNTHASE 1 (P5CS1) and downregulation of PROLINE DEHYDROGENASE 1 (PDH1) genes, which control proline biosynthetic and catabolic pathways, respectively. Chromatin immunoprecipitation and electrophoretic mobility shift assays demonstrated that the transcription factor ELONGATED HYPOCOTYL 5 (HY5) binds to G-box and C-box elements of P5CS1 and a C-box motif of PDH1. Salt-induced proline accumulation and P5CS1 expression were reduced in the hy5hyh double mutant, suggesting that HY5 promotes proline biosynthesis through connecting light and stress signals. Our results improve our understanding on interactions between stress and light signals, confirming HY5 as a key regulator in proline metabolism
Prognosis in human glioblastoma based on expression of ligand growth hormone-releasing hormone, pituitary-type growth hormone-releasing hormone receptor, its splicing variant receptors, EGF receptor and PTEN genes
Purpose G lioblastoma (GB) is the most frequent brain
tumor. Despite recent improvement in therapeutic strategies,
the prognosis of GB remains poor. Growth hormone-releasing
hormone (GHRH) may act as a growth factor; antagonists
of GHRH have been successfully applied for experimental
treatment of different types of tumors. The expression profile
of GHRH receptor, its main splice variant SV1 and GHRH
have not been investigated in human GB tissue samples.
Methods We examined the expression of GHRH, fulllength
pituitary-type GHRH receptor (pGHRHR), its functional
splice variant SV1 and non-functional SV2 by RTPCR
in 23 human GB specimens. Epidermal growth factor
receptor (EGFR) and phosphatase and tensin homolog gene
(PTEN) expression levels were also evaluated by quantitative
RT-PCR. Correlations between clinico-pathological
parameters and gene expressions were analyzed.
Results E xpression of GHRH was found to be positive
in 61.9 % of samples. pGHRH receptor was not expressed
in our sample set, while SV1 could be detected in 17.4 %
and SV2 in 8.6 % of the GB tissues. In 65.2 and 78.3 %
of samples, significant EGFR over-expression or PTEN
under-representation could be detected, respectively. In
47.8 % of cases, EGFR up-regulation and PTEN down-regulation
occurred together. Survival was significantly poorer
in tumors lacking GHRH expression. This worse prognosis
in GHRH negative group remained significant even if SV1
was also expressed.
Conclusion Our study shows that GHRH and SV1 genes
expressed in human GB samples and their expression patterns
are associated with poorer prognosis
Synthesis and direct assay of large macrocycle diversities by combinatorial late-stage modification at picomole scale
Macrocycles have excellent potential as therapeutics due to their ability to bind challenging targets. However, generating macrocycles against new targets is hindered by a lack of large macrocycle libraries for high-throughput screening. To overcome this, we herein established a combinatorial approach by tethering a myriad of chemical fragments to peripheral groups of structurally diverse macrocyclic scaffolds in a combinatorial fashion, all at a picomole scale in nanoliter volumes using acoustic droplet ejection technology. In a proof-of-concept, we generate a target-tailored library of 19,968 macrocycles by conjugating 104 carboxylic-acid fragments to 192 macrocyclic scaffolds. The high reaction efficiency and small number of side products of the acylation reactions allowed direct assay without purification and thus a large throughput. In screens, we identify nanomolar inhibitors against thrombin (Kiâ=â44â±â1ânM) and the MDM2:p53 protein-protein interaction (Kd MDM2â=â43â±â18ânM). The increased efficiency of macrocycle synthesis and screening and general applicability of this approach unlocks possibilities for generating leads against any protein target
TERT promoter mutations are highly recurrent in SHH subgroup medulloblastoma
Telomerase reverse transcriptase (TERT) promoter mutations were recently shown to drive telomerase activity in various cancer types, including medulloblastoma. However, the clinical and biological implications of TERT mutations in medulloblastoma have not been described. Hence, we sought to describe these mutations and their impact in a subgroup-specific manner. We analyzed the TERT promoter by direct sequencing and genotyping in 466 medulloblastomas. The mutational distributions were determined according to subgroup affiliation, demographics, and clinical, prognostic, and molecular features. Integrated genomics approaches were used to identify specific somatic copy number alterations in TERT promoter-mutated and wild-type tumors. Overall, TERT promoter mutations were identified in 21 % of medulloblastomas. Strikingly, the highest frequencies of TERT mutations were observed in SHH (83 %; 55/66) and WNT (31 %; 4/13) medulloblastomas derived from adult patients. Group 3 and Group 4 harbored this alteration in <5 % of cases and showed no association wit