142 research outputs found
The shape distribution of asteroid families -- evidence for evolution driven by small impacts
A statistical analysis of brightness variability of asteroids reveals how
their shapes evolve from elongated to rough spheroidal forms, presumably driven
by impact-related phenomena. Based on the Sloan Digital Sky Survey Moving
Object Catalog, we determined the shape distribution of 11,735 asteroids, with
special emphasis on eight prominent asteroid families. In young families,
asteroids have a wide range of shape elongations, implying
fragmentation-formation. In older families we see an increasing number of rough
spheroids, in agreement with the predictions of an impact-driven evolution. Old
families also contain a group of moderately elongated members, which we suggest
correspond to higher-density, more impact-resistant cores of former fragmented
asteroids that have undergone slow shape erosion. A few percent of asteroids
have very elongated shapes, and can either be young fragments or tidally
reshaped bodies. Our results confirm that the majority of asteroids are
gravitationally bound ``rubble piles''.Comment: Accepted by Icarus. 22 pages, 1 table, 6 figures, 31 figure panel
The amplitude of solar oscillations using stellar techniques
The amplitudes of solar-like oscillations depend on the excitation and
damping, both of which are controlled by convection. Comparing observations
with theory should therefore improve our understanding of the underlying
physics. However, theoretical models invariably compute oscillation amplitudes
relative to the Sun, and it is therefore vital to have a good calibration of
the solar amplitude using stellar techniques. We have used daytime spectra of
the Sun, obtained with HARPS and UCLES, to measure the solar oscillations and
made a detailed comparison with observations using the BiSON helioseismology
instrument. We find that the mean solar amplitude measured using stellar
techniques, averaged over one full solar cycle, is 18.7 +/- 0.7 cm/s for the
strongest radial modes (l=0) and 25.2 +/- 0.9 cm/s for l=1. In addition, we use
simulations to establish an equation that estimates the uncertainty of
amplitude measurements that are made of other stars, given that the mode
lifetime is known. Finally, we also give amplitudes of solar-like oscillations
for three stars that we measured from a series of short observations with HARPS
(gamma Ser, beta Aql and alpha For), together with revised amplitudes for five
other stars for which we have previously published results (alpha Cen A, alpha
Cen B, beta Hyi, nu Ind and delta Pav).Comment: 8 pages, accepted by ApJ. Minor wording changes and added a referenc
Solar-like oscillations in the G2 subgiant beta Hydri from dual-site observations
We have observed oscillations in the nearby G2 subgiant star beta Hyi using
high-precision velocity observations obtained over more than a week with the
HARPS and UCLES spectrographs. The oscillation frequencies show a regular comb
structure, as expected for solar-like oscillations, but with several l=1 modes
being strongly affected by avoided crossings. The data, combined with those we
obtained five years earlier, allow us to identify 28 oscillation modes. By
scaling the large frequency separation from the Sun, we measure the mean
density of beta Hyi to an accuracy of 0.6%. The amplitudes of the oscillations
are about 2.5 times solar and the mode lifetime is 2.3 d. A detailed comparison
of the mixed l=1 modes with theoretical models should allow a precise estimate
of the age of the star.Comment: 13 pages, 14 figures, accepted by ApJ. Fixed minor typo (ref to Fig
14
PloS one
In eukaryotes the TFIID complex is required for preinitiation complex assembly which positions RNA polymerase II around transcription start sites. On the other hand, histone acetyltransferase complexes including SAGA and ATAC, modulate transcription at several steps through modification of specific core histone residues. In this study we investigated the function of Drosophila melanogaster proteins TAF10 and TAF10b, which are subunits of dTFIID and dSAGA, respectively. We generated a mutation which eliminated the production of both Drosophila TAF10 orthologues. The simultaneous deletion of both dTaf10 genes impaired the recruitment of the dTFIID subunit dTAF5 to polytene chromosomes, while binding of other TFIID subunits, dTAF1 and RNAPII was not affected. The lack of both dTAF10 proteins resulted in failures in the larval-pupal transition during metamorphosis and in transcriptional reprogramming at this developmental stage. Surprisingly, unlike dSAGA mutations, dATAC subunit mutations resulted in very similar changes in the steady state mRNA levels of approximately 5000 genes as did ablation of both dTaf10 genes, indicating that dTAF10- and/or dTAF10b-containing complexes and dATAC affect similar pathways. Importantly, the phenotype resulting from dTaf10+dTaf10b mutation could be rescued by ectopically added ecdysone, suggesting that dTAF10- and/or dTAF10b-containing complexes are involved in the expression of ecdysone biosynthetic genes. Indeed, in dTaf10+dTaf10b mutants, cytochrome genes, which regulate ecdysone synthesis in the ring gland, were underrepresented. Therefore our data support the idea that the presence of dTAF10 proteins in dTFIID and/or dSAGA is required only at specific developmental steps. We propose that distinct forms of dTFIID and/or dSAGA exist during Drosophila metamorphosis, wherein different TAF compositions serve to target RNAPII at different developmental stages and tissues
The peak-flux of GRB 221009A measured with GRBAlpha
The brightest gamma-ray burst ever observed, long-duration GRB 221009A, was
detected by GRBAlpha nano-satellite without saturation. We present light curves
of the prompt emission in 13 energy bands, from 80 keV to 950 keV, and perform
a spectral analysis to calculate the peak flux and peak isotropic-equivalent
luminosity. Since the satellite's attitude information is not available for the
time of this GRB, more than 200 incident directions were probed in order to
find the median luminosity and its systematic uncertainty. We found that the
peak flux in the keV range (observer frame) was
ph cms or
erg cms
and the fluence in the same energy range of the first GRB episode lasting 300
s, which was observable by GRBAlpha, was erg
cm or erg cm for
the extrapolated range of keV. We infer the isotropic-equivalent
released energy of the first GRB episode to be
erg in the
keV band (rest frame at ). The peak isotropic-equivalent luminosity in
the keV range (rest frame) was
erg s and the
bolometric peak isotropic-equivalent luminosity was
erg s (4 s
scale) in the keV range (rest frame). The peak emitted energy is
keV. Our measurement of
is consistent with the Yonetoku relation. It is
possible that, due to the spectral evolution of this GRB and orientation of
GRBAlpha at the peak time, the true values of peak flux, fluence,
, and are even higher. [abridged]Comment: 7 pages, 7 figures, 1 table, accepted for publication in Astronomy &
Astrophysic
Experimental identification and analysis of macronuclear non-coding RNAs from the ciliate Tetrahymena thermophila
The ciliate Tetrahymena thermophila is an important eukaryotic model organism that has been used in pioneering studies of general phenomena, such as ribozymes, telomeres, chromatin structure and genome reorganization. Recent work has shown that Tetrahymena has many classes of small RNA molecules expressed during vegetative growth or sexual reorganization. In order to get an overview of medium-sized (40–500 nt) RNAs expressed from the Tetrahymena genome, we created a size-fractionated cDNA library from macronuclear RNA and analyzed 80 RNAs, most of which were previously unknown. The most abundant class was small nucleolar RNAs (snoRNAs), many of which are formed by an unusual maturation pathway. The modifications guided by the snoRNAs were analyzed bioinformatically and experimentally and many Tetrahymena-specific modifications were found, including several in an essential, but not conserved domain of ribosomal RNA. Of particular interest, we detected two methylations in the 5′-end of U6 small nuclear RNA (snRNA) that has an unusual structure in Tetrahymena. Further, we found a candidate for the first U8 outside metazoans, and an unusual U14 candidate. In addition, a number of candidates for new non-coding RNAs were characterized by expression analysis at different growth conditions
SNOntology: Myriads of novel snornas or just a mirage?
<p>Abstract</p> <p>Background</p> <p>Small nucleolar RNAs (snoRNAs) are a large group of non-coding RNAs (ncRNAs) that mainly guide 2'-O-methylation (C/D RNAs) and pseudouridylation (H/ACA RNAs) of ribosomal RNAs. The pattern of rRNA modifications and the set of snoRNAs that guide these modifications are conserved in vertebrates. Nearly all snoRNA genes in vertebrates are localized in introns of other genes and are processed from pre-mRNAs. Thus, the same promoter is used for the transcription of snoRNAs and host genes.</p> <p>Results</p> <p>The series of studies by Dahai Zhu and coworkers on snoRNAs and their genes were critically considered. We present evidence that dozens of species-specific snoRNAs that they described in vertebrates are experimental artifacts resulting from the improper use of Northern hybridization. The snoRNA genes with putative intrinsic promoters that were supposed to be transcribed independently proved to contain numerous substitutions and are, most likely, pseudogenes. In some cases, they are localized within introns of overlooked host genes. Finally, an increased number of snoRNA genes in mammalian genomes described by Zhu and coworkers is also an artifact resulting from two mistakes. First, numerous mammalian snoRNA pseudogenes were considered as genes, whereas most of them are localized outside of host genes and contain substitutions that question their functionality. Second, Zhu and coworkers failed to identify many snoRNA genes in non-mammalian species. As an illustration, we present 1352 C/D snoRNA genes that we have identified and annotated in vertebrates.</p> <p>Conclusions</p> <p>Our results demonstrate that conclusions based only on databases with automatically annotated ncRNAs can be erroneous. Special investigations aimed to distinguish true RNA genes from their pseudogenes should be done. Zhu and coworkers, as well as most other groups studying vertebrate snoRNAs, give new names to newly described homologs of human snoRNAs, which significantly complicates comparison between different species. It seems necessary to develop a uniform nomenclature for homologs of human snoRNAs in other vertebrates, e.g., human gene names prefixed with several-letter code denoting the vertebrate species.</p
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