Investigating Bodily Injury

This study shall analyse the statutory provisions on bodily injuries or batteries with a special outlook to the difficulties caused by the pandemic. The study shall introduce the relations of corona virus and HIV virus to bodily injuries. Interpretation of the recommendations of criminalistics is indispensable for the detection of bodily injuries, similarly, the identification of the methods revealing criminal actions and the questions to be asked during the interrogation of the suspect are also absolute necessities in the process

Investigating Bodily Injury

This study shall analyse the statutory provisions on bodily injuries or batteries with a special outlook to the difficulties caused by the pandemic. The study shall introduce the relations of corona virus and HIV virus to bodily injuries. Interpretation of the recommendations of criminalistics is indispensable for the detection of bodily injuries, similarly, the identification of the methods revealing criminal actions and the questions to be asked during the interrogation of the suspect are also absolute necessities in the process

Investigating Bodily Injury

This study shall analyse the statutory provisions on bodily injuries or batteries with a special outlook on the difficulties caused by the pandemic. The study formulates forensic recommendations based on the Hungarian national standard and knowledge of criminal procedure and analyzes the methodology of the investigation of bodily harm exclusively from the Hungarian point of view. It approaches the detection of the crime from a practical and empirical point of view, as well as from a legal point of view. The study shall introduce the relations of corona virus and HIV virus to bodily injuries. Interpretation of the recommendations of criminalistics is indispensable for the detection of bodily injuries, similarly, the identification of the methods revealing criminal actions and the questions to be asked during the interrogation of the suspect are also absolute necessities in the process

Un Ballo in maschera

De cada obra s'ha digitalitzat un programa sencer. De la resta s'han digitalitzat les parts que són diferents.Direcció: Laszlo HalaszEmpresa: Juan A. PamiasÒpera de Giuseppe Verdi, amb llibret d'Antonio Somm

Andrea Chenier

De cada obra s'ha digitalitzat un programa sencer. De la resta s'han digitalitzat les parts que són diferents.Direcció: Laszlo HalaszEmpresa: Juan A. Pamia

Tristan e Isolda

Director Halasz LaszloDrama líric en tres actes, llibret i música de R. WagnerEmpresa J.F. ArquerFunció a benefici dels empleats permanents, acomodadors i porters del G.T. del LiceuMestre de Cor : Vittorio Barbieri ; Decorats : J. Mestres i Cabane

The transcriptional control of the VEGFA-VEGFR1 (FLT1) axis in alternatively polarized murine and human macrophages

Introduction: Macrophages significantly contribute to the regulation of vessel formation under physiological and pathological conditions. Although the angiogenesis-regulating role of alternatively polarized macrophages is quite controversial, a growing number of evidence shows that they can participate in the later phases of angiogenesis, including vessel sprouting and remodeling or regression. However, the epigenetic and transcriptional regulatory mechanisms controlling this angiogenesis-modulating program are not fully understood. Results: Here we show that IL-4 can coordinately regulate the VEGFA-VEGFR1 (FLT1) axis via simultaneously inhibiting the proangiogenic Vegfa and inducing the antiangiogenic Flt1 expression in murine bone marrow-derived macrophages, which leads to the attenuated proangiogenic activity of alternatively polarized macrophages. The IL-4-activated STAT6 and IL-4-STAT6 signaling pathway-induced EGR2 transcription factors play a direct role in the transcriptional regulation of the Vegfa-Flt1 axis. We demonstrated that this phenomenon is not restricted to the murine bone marrow-derived macrophages, but can also be observed in different murine tissue-resident macrophages ex vivo and parasites-elicited macrophages in vivo with minor cell type-specific differences. Furthermore, IL-4 exposure can modulate the hypoxic response of genes in both murine and human macrophages leading to a blunted Vegfa/VEGFA and synergistically induced Flt1/FLT1 expression. Discussion: Our findings establish that the IL-4-activated epigenetic and transcriptional program can determine angiogenesis-regulating properties in alternatively polarized macrophages under normoxic and hypoxic conditions

Orfeo

Coreògraf i mestre de ball Joan MagrinyàDe cada obra s'ha digitalitzat un programa sencer. De la resta s'han digitalitzat les parts que són diferents.Empresa: Juan A. PamiasOrquestra del Gran Teatre del Liceu dirigida per Laszlo HalaszÒpera de Gluck i llibret de Calzabig

The transcription factor EGR2 is the molecular linchpin connecting STAT6 activation to the late, stable epigenomic program of alternative macrophage polarization

Macrophages polarize into functionally distinct subtypes while responding to microenvironmental cues. The identity of proximal transcription factors (TFs) downstream from the polarization signals are known, but their activity is typically transient, failing to explain the long-term, stable epigenomic programs developed. Here, we mapped the early and late epigenomic changes of interleukin-4 (IL-4)-induced alternative macrophage polarization. We identified the TF, early growth response 2 (EGR2), bridging the early transient and late stable gene expression program of polarization. EGR2 is a direct target of IL-4-activated STAT6, having broad action indispensable for 77% of the induced gene signature of alternative polarization, including its autoregulation and a robust, downstream TF cascade involving PPARG. Mechanistically, EGR2 binding results in chromatin opening and the recruitment of chromatin remodelers and RNA polymerase II. Egr2 induction is evolutionarily conserved during alternative polarization of mouse and human macrophages. In the context of tissue resident macrophages, Egr2 expression is most prominent in the lung of a variety of species. Thus, EGR2 is an example of an essential and evolutionarily conserved broad acting factor, linking transient polarization signals to stable epigenomic and transcriptional changes in macrophages

The Epigenetic State of IL-4-Polarized Macrophages Enables Inflammatory Cistromic Expansion and Extended Synergistic Response to TLR Ligands

Prior exposure to microenvironmental signals could fundamentally change the response of macrophages to subsequent stimuli. It is believed that T helper-2 (Th2)-cell-type cytokine interleukin-4 (IL-4) and Toll-like receptor (TLR) ligand-activated transcriptional programs mutually antagonize each other, and no remarkable convergence has been identified between them. In contrast, here, we show that IL-4-polarized macrophages established a hyperinflammatory gene expression program upon lipopolysaccharide (LPS) exposure. This phenomenon, which we termed extended synergy, was supported by IL-4-directed epigenomic remodeling, LPS-activated NF-κB-p65 cistrome expansion, and increased enhancer activity. The EGR2 transcription factor contributed to the extended synergy in a macrophage-subtype-specific manner. Consequently, the previously alternatively polarized macrophages produced increased amounts of immune-modulatory factors both in vitro and in vivo in a murine Th2 cell-type airway inflammation model upon LPS exposure. Our findings establish that IL-4-induced epigenetic reprogramming is responsible for the development of inflammatory hyperresponsiveness to TLR activation and contributes to lung pathologies