Holocene climate variability, vegetation dynamics and fire regime in the central Pyrenees: the Basa de la Mora sequence (NE Spain)

High resolution multiproxy data (pollen, sedimentology, geochemistry, chironomids and charcoal) from the Basa de la Mora (BSM) lake sequence (42° 32′ N, 0° 19′ E, 1914 m a.s.l.) show marked climate variability in the central southern Pyrenees throughout the Holocene. A robust age model based on 15 AMS radiocarbon dates underpins the first precise reconstruction of rapid climate changes during the Holocene from this area. During the Early Holocene, increased winter snowpack and high snowmelt during summer, as a consequence of high seasonality, led to higher lake levels, a chironomid community dominated by non-lacustrine taxa (Orthocladiinae) related to higher inlet streams, and a forested landscape with intense run-off processes in the watershed. From 9.8 to 8.1 cal ka BP, climate instability is inferred from rapid and intense forest shifts and high fluctuation in surface run-off. Shifts among conifers and mesophytes reveal at least four short-lived dry events at 9.7, 9.3, 8.8 and 8.3 cal ka BP. Between 8.1 and 5.7 cal ka BP a stable climate with higher precipitation favoured highest lake levels and forest expansion, with spread of mesophytes, withdrawal of conifers and intensification of fires, coinciding with the Holocene Climate Optimum. At 5.7 cal ka BP a major change leading to drier conditions contributed to a regional decline in mesophytes, expansion of pines and junipers, and a significant lake level drop. Despite drier conditions, fire activity dropped as consequence of biomass reduction. Two arid intervals occurred between 2.9 and 2.4 cal ka BP and at 1.2–0.7 cal ka BP (800–1300 AD). The latter coincides with the Medieval Climate Anomaly and is one of the most arid phases of the Holocene in BSM sequence. Anthropogenic disturbances were small until 700 AD, when human pressure over landscape intensified, with Olea cultivation in the lowlands and significant deforestation in highlands. Colder and unfavourable weather conditions during the second part of the Little Ice Age caused a temporary cease of high-land management. The most intense anthropogenic disturbances occurred during the second half of 19th century. Last decades are characterized by recovery of the vegetation cover as a result of land abandonment, and lowered lake levels, probably due to higher temperatures

Higher COVID-19 pneumonia risk associated with anti-IFN-α than with anti-IFN-ω auto-Abs in children

We found that 19 (10.4%) of 183 unvaccinated children hospitalized for COVID-19 pneumonia had autoantibodies (auto-Abs) neutralizing type I IFNs (IFN-alpha 2 in 10 patients: IFN-alpha 2 only in three, IFN-alpha 2 plus IFN-omega in five, and IFN-alpha 2, IFN-omega plus IFN-beta in two; IFN-omega only in nine patients). Seven children (3.8%) had Abs neutralizing at least 10 ng/ml of one IFN, whereas the other 12 (6.6%) had Abs neutralizing only 100 pg/ml. The auto-Abs neutralized both unglycosylated and glycosylated IFNs. We also detected auto-Abs neutralizing 100 pg/ml IFN-alpha 2 in 4 of 2,267 uninfected children (0.2%) and auto-Abs neutralizing IFN-omega in 45 children (2%). The odds ratios (ORs) for life-threatening COVID-19 pneumonia were, therefore, higher for auto-Abs neutralizing IFN-alpha 2 only (OR [95% CI] = 67.6 [5.7-9,196.6]) than for auto-Abs neutralizing IFN-. only (OR [95% CI] = 2.6 [1.2-5.3]). ORs were also higher for auto-Abs neutralizing high concentrations (OR [95% CI] = 12.9 [4.6-35.9]) than for those neutralizing low concentrations (OR [95% CI] = 5.5 [3.1-9.6]) of IFN-omega and/or IFN-alpha 2

Historia del fuego y la vegetación en una secuencia Holocena del Pirineo Central: La Basa de la Mora

[EN] The importance of studying past fire regimes is derived from the need to understand the effects of current Global Change on present and future fire activity. Studying the relationship between long-term fire ecology, ecosystems and climate allows us to understand how vegetation responds to this perturbation and to define the thresholds triggering irreversible changes in the vegetation. This work presents the last 10 000 years of vegetation change and fire activity on a high altitude ecosystem from the Pyrenees range: the mountain lake Basa de la Mora located in the central southern Pyrenees. Holocene fire activity has been inferred through fossil carbon particle identification in the sedimentary sequence obtained in this lake. We observe that the strongest Holocene fire activity in the area occurred between 8.1 and 4 cal Kyr BP. This period was followed by a phase, between 3.2 and 1.7 cal Kyr BP, where almost no fire activity was recorded. These changes seem to be due to biomass availability and fire-conductive climate conditions. Positive correlation has been found between fire activity and Betula, Corylus, as the key fueling taxa and groups of ariditytolerant taxa. Additionally, fire dynamics seems coupled to higher anthropogenic activities after 1.7 cal Kyr BP. © Universidad de La Rioja.[ES] La importancia del estudio de la actividad del fuego en el pasado está en la necesidad de comprender los efectos que el Cambio Global puede tener sobre la misma, tanto en la actualidad como en el futuro. Estudiar cómo se relaciona el fuego con los ecosistemas y el clima en escalas temporales lo suficientemente largas permite conocer la respuesta de la vegetación a esta perturbación y definir los umbrales que provocan cambios irreversibles en la composición vegetal. Este trabajo estudia la dinámica del fuego durante el Holoceno en el entorno de la Basa de la Mora, lago de origen glaciar situado en el Macizo de Cotiella, Pirineo Central. La historia del fuego se ha reconstruido a partir de la identificación de partículas de carbón fósil a lo largo de la secuencia sedimentaria extraída del lago, que cubre los últimos 10 000 años de historia. Se ha observado que la mayor actividad del fuego se produce entre 8.1 y 4 cal Ka BP, seguida de una fase de menor actividad entre 3.2 y 1.7 cal Ka BP. Estas fluctuaciones se deben principalmente a dos razones: la cantidad de biomasa disponible y susceptible de ser quemada, y la existencia de un clima favorable para la ocurrencia de incendios. En ese sentido, se han observado correlaciones positivas de la actividad del fuego, por un lado, con Betula y Corylus como taxones ligados a la mayor producción de biomasa, y por otro, con agrupaciones de taxones adaptados a la aridez como indicadores de climas favorables a la ocurrencia de incendios. Por último, a partir de 1.7 cal Ka BP se registra en la secuencia una mayor incidencia del fuego, probablemente vinculada a la intensificación de la acción antropogénica a nivel regional.Esta investigación ha sido financiada con los proyectos ARAFIRE Convenio DGAla Caixa ref. 2012 GA LC 064, DINAMO ref. CGL2009-07992, GRACCIE-CONSOLIDER ref. CSD2007-00067 y HORDA-OAPN ref: 83/2009.Peer Reviewe

Holocene climate variability, vegetation dynamics and fire regime in the central Pyrenees: The Basa de la Mora sequence (NE Spain)

[EN] High resolution multiproxy data (pollen, sedimentology, geochemistry, chironomids and charcoal) from the Basa de la Mora (BSM) lake sequence (42°32' N, 0°19' E, 1914m a.s.l.) show marked climate variability in the central southern Pyrenees throughout the Holocene. A robust age model based on 15 AMS radiocarbon dates underpins the first precise reconstruction of rapid climate changes during the Holocene from this area. During the Early Holocene, increased winter snowpack and high snowmelt during summer, as a consequence of high seasonality, led to higher lake levels, a chironomid community dominated by non-lacustrine taxa (Orthocladiinae) related to higher inlet streams, and a forested landscape with intense run-off processes in the watershed. From 9.8 to 8.1calkaBP, climate instability is inferred from rapid and intense forest shifts and high fluctuation in surface run-off. Shifts among conifers and mesophytes reveal at least four short-lived dry events at 9.7, 9.3, 8.8 and 8.3calkaBP. Between 8.1 and 5.7calkaBP a stable climate with higher precipitation favoured highest lake levels and forest expansion, with spread of mesophytes, withdrawal of conifers and intensification of fires, coinciding with the Holocene Climate Optimum. At 5.7calkaBP a major change leading to drier conditions contributed to a regional decline in mesophytes, expansion of pines and junipers, and a significant lake level drop. Despite drier conditions, fire activity dropped as consequence of biomass reduction. Two arid intervals occurred between 2.9 and 2.4calkaBP and at 1.2-0.7calkaBP (800-1300 AD). The latter coincides with the Medieval Climate Anomaly and is one of the most arid phases of the Holocene in BSM sequence. Anthropogenic disturbances were small until 700 AD, when human pressure over landscape intensified, with Olea cultivation in the lowlands and significant deforestation in highlands. Colder and unfavourable weather conditions during the second part of the Little Ice Age caused a temporary cease of high-land management. The most intense anthropogenic disturbances occurred during the second half of 19th century. Last decades are characterized by recovery of the vegetation cover as a result of land abandonment, and lowered lake levels, probably due to higher temperatures. © 2013 Elsevier Ltd.Financial support for research was provided by the former Spanish Inter-Ministry Commission of Science and Technology (CICIYT) through the projects DINAMO (CGL2009-07992), DINAMO2 (CGL2012-33063), GRACCIE-CONSOLIDER (CSD2007-00067) and HORDA (83/2008), from Parques Nacionales. Additional funding support has been provided by the Aragon Government through the project PM073/2007 and by Geoparque del Sobrarbe through the project “High resolution chronological control of Basa de la Mora”. Ana Pérez-Sanz has been supported by a PhD Fellowship provided by the Aragon Government. Ana Moreno, Graciela Gil-Romera and Mario Morellón hold post-doctoral contracts funded by the “Ramón y Cajal”, “Juan de la Cierva” and “JAE-DOC CSIC” programs, respectively. We thank to Santiago Giralt, Alberto Sáez, Armand Hernández, Carlos Martí, Mayte Rico, Juan Pablo Corella and Antonio Vallejo for coring assistance in 2008. We also thank Beatriz Bueno and Aída Adsuar for their help in lab procedures. We are indebted to Prof. Sandy Harrison for her assistance with the English review that has led to a noticeable improvement of the manuscript.Peer Reviewe

Neddylation tunes peripheral blood mononuclear cells immune response in COVID-19 patients

The COVID-19 pandemic caused by SARS-CoV-2 has reached 5.5 million deaths worldwide, generating a huge impact globally. This highly contagious viral infection produces a severe acute respiratory syndrome that includes cough, mucus, fever and pneumonia. Likewise, many hospitalized patients develop severe pneumonia associated with acute respiratory distress syndrome (ARDS), along an exacerbated and uncontrolled systemic inflammation that in some cases induces a fatal cytokine storm. Although vaccines clearly have had a beneficial effect, there is still a high percentage of unprotected patients that develop the pathology, due to an ineffective immune response. Therefore, a thorough understanding of the modulatory mechanisms that regulate the response to SARS-CoV-2 is crucial to find effective therapeutic alternatives. Previous studies describe the relevance of Neddylation in the activation of the immune system and its implications in viral infection. In this context, the present study postulates Neddylation, a reversible ubiquitin-like post-translational modification of proteins that control their stability, localization and activity, as a key regulator in the immune response against SARS-CoV-2. For the first time, we describe an increase in global neddylation levels in COVID-19 in the serum of patients, which is particularly associated with the early response to infection. In addition, the results showed that overactivation of neddylation controls activation, proliferation, and response of peripheral blood mononuclear cells (PBMCs) isolated from COVID-19 patients. Inhibition of neddylation, and the subsequent avoidance of activated PBMCs, reduces cytokine production, mainly IL-6 and MCP-1 and induce proteome modulation, being a critical mechanism and a potential approach to immunomodulate COVID-19 patients.Acknowledgements: This work was supported by the Ministry of Economy, Industry and Competitiveness PID2020-117116RB-I0, PID2019-107956RA-I00 integrado en el Plan Estatal de Investigación Cientifica y Técnica y Innovación, cofinanciado con Fondos FEDER to (MLM-C, AP); Subprograma Retos Colaboración RTC2019-007125-1 to MLM-C; La Caixa Foundation Program HR17-00601 (to MLM-CH), Proyectos Investigacion en Salud DTS20/00138 (to MLM-CH) and DTS21/00094 (to AP), Departamento de Industria del Gobierno Vasco (to MLM-CH); Ciberehd_ISCIII_ is funded by the Instituto de Salud Carlos III. This work was partially supported by the Cantabrian Government (grant number 2020UIC22-PUB-001), and by Instituto de Salud Carlos III (grant number COV20/00170 to ML-H). ERC Starting grant 804236 NEXTGEN-IO (to AP), Ayuda RYC2018-024183-I financiada por MCIN/AEI416 /10.13039/501100011033 y por El FSE invierte en tu futuro (to AP). PhD fellowship from Asociación Española contra el Cáncer (PRDVZ172010SERR and PRDVZ19003BOSC) awarded to MS-M and AB. PhD fellowship from Gobierno Vasco awarded to NG-U, SL, and BJ-L (PRE_2020_2_0154). Finally, we would like to acknowledge Begoña Rodríguez Iruretagoyena for the technical support provided