Museum augmented interface for historical scale models: towards a new way for cultural heritage promotion

International audienceIn this paper, we describe an interactive museum application dedicated to historical scale models. This comes from a joint work between multidisciplinary teams: industrial engineering researchers, historians, museum curators and interactive interface designers. We present here theresult of the project, based on scientific methodology. Results include system architecture, hardware and software, some use cases and user evaluation figures. This paper also underlines some methodology issues that illustrate future possibilities

Nantes 1900 - la maquette du port: Une valorisation scientifique et musicographique innovante

National audienceLe plan-relief du port de Nantes, présenté à l’Exposition universelle de 1900 à Paris, intègre à la fin des années 1920 les collections des musées nantais : objet promotionnel pendant deux décennies au service de l’excellence des aménagements portuaires, il devient dès lors un objet patrimonial d’exception.Son insertion dans le parcours scénographique du musée d’histoire de Nantes amène rapidement les équipes de la conservation à porter un nouveau regard sur cette maquette, à la questionner et à imaginer une médiation pour « la faire parler ».Très vite, une collaboration entre l’Université, l’École Centrale de Nantes et le musée voit le jour afin de mettre en place un outil numérique issu de champs de recherches variés.Désormais, par ce dispositif innovant mis à la disposition du public, le plan-relief devient, au-delà d’un précieux indicateur de l’histoire de l’architecture industrielle et des paysages nantais du début du 20e siècle, un support dynamique à la compréhension du territoire actuel, au cœur de la réflexion sur le devenir de Nantes et de son rapport à la Loire

High-resolution modal analysis

Usual modal analysis techniques are based on the Fourier transform. Due to the Delta T . Delta f limitation, they perform poorly when the modal overlap mu exceeds 30%. A technique based on a high-resolution analysis algorithm and an order-detection method is presented here, with the aim of filling the gap between the low- and the high-frequency domains (30%<mu<100%). A pseudo-impulse force is applied at points of interests of a structure and the response is measured at a given point. For each pair of measurements, the impulse response of the structure is retrieved by deconvolving the pseudo-impulse force and filtering the response with the result. Following conditioning treatments, the reconstructed impulse response is analysed in different frequency-bands. In each frequency-band, the number of modes is evaluated, the frequencies and damping factors are estimated, and the complex amplitudes are finally extracted. As examples of application, the separation of the twin modes of a square plate and the partial modal analyses of aluminium plates up to a modal overlap of 70% are presented. Results measured with this new method and those calculated with an improved Rayleigh method match closely

The DNA polymerase λ is required for the repair of non-compatible DNA double strand breaks by NHEJ in mammalian cells

DNA polymerase lambda (polλ) is a recently identified DNA polymerase whose cellular function remains elusive. Here we show, that polλ participates at the molecular level in a chromosomal context, in the repair of DNA double strand breaks (DSB) via non-homologous end joining (NHEJ) in mammalian cells. The expression of a catalytically inactive form of polλ (polλDN) decreases the frequency of NHEJ events in response to I-Sce-I-induced DSB whereas inactivated forms of its homologues polβ and polμ do not. Only events requiring DNA end processing before ligation are affected; this defect is associated with large deletions arising in the vicinity of the induced DSB. Furthermore, polλDN-expressing cells exhibit increased sensitization and genomic instability in response to ionizing radiation similar to that of NHEJ-defective cells. Our data support a requirement for polλ in repairing a subset of DSB in genomic DNA, thereby contributing to the maintenance of genetic stability mediated by the NHEJ pathway

How effective is the BNT162b2 mRNA vaccine against SARS-CoV-2 transmission and infection? A national programme analysis in Monaco, July 2021 to September 2022.

BackgroundWe quantified SARS-CoV-2 dynamics in different community settings and the direct and indirect effect of the BNT162b2 mRNA vaccine in Monaco for different variants of concern (VOC).MethodsBetween July 2021 and September 2022, we prospectively investigated 20,443 contacts from 6320 index cases using data from the Monaco COVID-19 Public Health Programme. We calculated secondary attack rates (SARs) in households (n = 13,877), schools (n = 2508) and occupational (n = 6499) settings. We used binomial regression with a complementary log-log link function to measure adjusted hazard ratios (aHR) and vaccine effectiveness (aVE) for index cases to infect contacts and contacts to be infected in households.ResultsIn households, the SAR was 55% (95% CI 54-57) and 50% (48-51) among unvaccinated and vaccinated contacts, respectively. The SAR was 32% (28-36) and 12% (10-13) in workplaces, and 7% (6-9) and 6% (3-10) in schools, among unvaccinated and vaccinated contacts respectively. In household, the aHR was lower in contacts than in index cases (aHR 0.68 [0.55-0.83] and 0.93 [0.74-1.1] for delta; aHR 0.73 [0.66-0.81] and 0.89 [0.80-0.99] for omicron BA.1&2, respectively). Vaccination had no significant effect on either direct or indirect aVE for omicron BA.4&5. The direct aVE in contacts was 32% (17, 45) and 27% (19, 34), and for index cases the indirect aVE was 7% (- 17, 26) and 11% (1, 20) for delta and omicron BA.1&2, respectively. The greatest aVE was in contacts with a previous SARS-CoV-2 infection and a single vaccine dose during the omicron BA.1&2 period (45% [27, 59]), while the lowest were found in contacts with either three vaccine doses (aVE - 24% [- 63, 6]) or one single dose and a previous SARS-CoV-2 infection (aVE - 36% [- 198, 38]) during the omicron BA.4&5 period.ConclusionsProtection conferred by the BNT162b2 mRNA vaccine against transmission and infection was low for delta and omicron BA.1&2, regardless of the number of vaccine doses and previous SARS-CoV-2 infection. There was no significant vaccine effect for omicron BA.4&5. Health authorities carrying out vaccination campaigns should bear in mind that the current generation of COVID-19 vaccines may not represent an effective tool in protecting individuals from either transmitting or acquiring SARS-CoV-2 infection

Proteome changes driven by phosphorus deficiency and recovery in the brown tide-forming alga Aureococcus anophagefferens

© The Author(s), 2011. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 6 (2011): e28949, doi:10.1371/journal.pone.0028949.Shotgun mass spectrometry was used to detect proteins in the harmful alga, Aureococcus anophagefferens, and monitor their relative abundance across nutrient replete (control), phosphate-deficient (−P) and −P refed with phosphate (P-refed) conditions. Spectral counting techniques identified differentially abundant proteins and demonstrated that under phosphate deficiency, A. anophagefferens increases proteins involved in both inorganic and organic phosphorus (P) scavenging, including a phosphate transporter, 5′-nucleotidase, and alkaline phosphatase. Additionally, an increase in abundance of a sulfolipid biosynthesis protein was detected in −P and P-refed conditions. Analysis of the polar membrane lipids showed that cellular concentrations of the sulfolipid sulphoquinovosyldiacylglycerol (SQDG) were nearly two-fold greater in the −P condition versus the control condition, while cellular phospholipids were approximately 8-fold less. Transcript and protein abundances were more tightly coupled for gene products involved in P metabolism compared to those involved in a range of other metabolic functions. Comparison of protein abundances between the −P and P-refed conditions identified differences in the timing of protein degradation and turnover. This suggests that culture studies examining nutrient starvation responses will be valuable in interpreting protein abundance patterns for cellular nutritional status and history in metaproteomic datasets.Research for this work was supported by a National Oceanic and Atmospheric Administration ECOHAB grant (#NA09NOS4780206) and National Science Foundation grant (#OCE-0723667) and a STAR Research Assistance Agreement No. R-83041501-0 awarded by the U.S. Environmental Protection Agency. Further support came from the Woods Hole Coastal Ocean Institute. LLW was supported by a Environmental Protection Agency STAR Fellowship (#FP916901). EMB was supported by a National Science Foundation (NSF) Graduate Research Fellowship (#2007037200) and an Environmental Protection Agency STAR Fellowship (#F6E20324)

Les apports et la mise à disposition des données sur les sols

absen

Du sol à la carte, la connaissance des sols par le programme « Inventaire Gestion et Conservation des sols » (IGCS)

- Introduction et contexte- Méthode de cartographie des sols- Programme d’inventaire : Inventaire Gestion et Conservation des Sols IGCS- Conclusion

Réferentiel régional pédologique de Bourgogne à 1/250 000 : Régions naturelles, pédopaysages et sols de la Saône-et-Loire

National audienc

Réferentiel régional pédologique de Bourgogne à 1/250 000 : Régions naturelles, pédopaysages et sols de la Saône-et-Loire

National audienc