Measures for diagnosing and treating infections by a novel coronavirus responsible for a pneumonia outbreak originating in Wuhan, China.

On 10 January 2020, a new coronavirus causing a pneumonia outbreak in Wuhan City in central China was denoted as 2019-nCoV by the World Health Organization (WHO). As of 24 January 2020, there were 887 confirmed cases of 2019-nCoV infection, including 26 deaths, reported in China and other countries. Therefore, combating this new virus and stopping the epidemic is a matter of urgency. Here, we focus on advances in research and development of fast diagnosis methods, as well as potential prophylactics and therapeutics to prevent or treat 2019-nCoV infection

Collaborating to safeguard children in Taiwan: Systemic transformation

Child abuse and exploitation pose significant threats to the health and well-being of children. While the Taiwanese government introduced the Protection of Children and Youth Welfare and Rights Act in 2011 to address these issues, progress has been slow. This paper aims to examine the evolution of Taiwan’s child protection system (CPS), with a particular focus on interdepartmental collaboration. Through the collection of legislation, statistics, conference proceedings, and reports, this study analyzes the working model between law enforcement and public health. Three cases of collaboration between law enforcement and public health at the community level are presented: social safety net programs, early intervention for child abuse, and trauma-informed training for first responders. The accomplishments and challenges of each project are discussed, along with a review of the CPS in relation to the United Nations (UN) strategy INSPIRE’s approaches. Although Taiwan has shown a commendable emphasis on prevention and family support, the collaboration between law enforcement and public health is still in its early stages. The next crucial step is to strengthen integration in the early stages of identifying, assessing, and referring cases of child abuse and neglect. This can be achieved by generating more evidence on effective working models and promoting their implementation

An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus

Background. A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus. Results. Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus. Conclusions. Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus. © 2010 Zhao et al; licensee BioMed Central Ltd.published_or_final_versio

A Splicing Mutation in Slc4a5 Results in Retinal Detachment and Retinal Pigment Epithelium Dysfunction

Fluid and solute transporters of the retinal pigment epithelium (RPE) are core components of the outer blood-retinal barrier. Characterizing these transporters and their role in retinal homeostasis may provide insights into ocular function and disease. Here, we describe RPE defects in tvrm77 mice, which exhibit hypopigmented patches in the central retina. Mapping and nucleotide sequencing of tvrm77 mice revealed a disrupted 5\u27 splice donor sequence in Slc4a5, a sodium bicarbonate cotransporter gene. Slc4a5 expression was reduced 19.7-fold in tvrm77 RPE relative to controls, and alternative splice variants were detected. SLC4A5 was localized to the Golgi apparatus of cultured human RPE cells and in apical and basal membranes. Fundus imaging, optical coherence tomography, microscopy, and electroretinography (ERG) of tvrm77 mice revealed retinal detachment, hypopigmented patches corresponding to neovascular lesions, and retinal folds. Detachment worsened and outer nuclear layer thickness decreased with age. ERG a- and b-wave response amplitudes were initially normal but declined in older mice. The direct current ERG fast oscillation and light peak were reduced in amplitude at all ages, whereas other RPE-associated responses were unaffected. These results link a new Slc4a5 mutation to subretinal fluid accumulation and altered light-evoked RPE electrophysiological responses, suggesting that SLC4A5 functions at the outer blood-retinal barrier

High-Permeability Wood with Microwave Remodeling Structure

This paper presents the development of a microwave puffed wood (MPW) with novel and unique structural features in its internal structure that are based on natural wood (NW). The focus of the research was on the comprehensive visualization of the structural characteristics of MPW from the macroscopic to the microscopic scale followed by an exploration of its impregnation capabilities. The results showed that the volume of MPW increased by about 9% compared to NW due to the presence of a large number of cracked cavities. The CT images indicated that there was a significant difference between the macroscopic cracks produced by microwave processing and the natural cracks in the wood. The mercury intrusion test results showed that macro-pores increased while the micro-pores decreased in the MPW compared to in the NW. The MPW showed good fluid permeability and liquid absorption performance. The phenolic resin penetration rate of the MPW was about 20 times that of the NW, and the material absorption was more than 2 times that of the NW. The crack space enabled the MPW to serve as a fluid transportation and a storage warehouse. MPW is a super container based on natural materials. It has broad potential in more fields, such as in wood composite materials

Experimental Study on Flexible Fiber Assisted Shear Thickening Polishing for Cutting Edge Preparation of Core Drill

To improve the cutting performance of the core drill, the flexible fiber assisted shear-thickening polishing (FF-STP) for cutting edge preparation was proposed to eliminate the microscopic defect and obtain proper radius of the cutting edge of the core drill. The flexible fiber was introduced into the shear-thickening polishing process to break the thickened agglomerates and improve the efficiency of cutting edge preparation. The influence of the polishing speed, abrasive concentration and the flexible fiber contact length with the core drill on the cutting edge radius r and surface morphology of the core drill edge was analyzed, and the drilling experiments were carried out after preparation, the cutting heat and drilled holes’ roughness were employed as evaluation indexes to evaluate the performance of the core drill. The results show that the cutting edge radius increases with the increase of polishing speed, abrasive concentration and contact length. However, too high a polishing speed and contact length reduce the abrasive particles involved in the polishing process, and then lead to a decline in preparation efficiency. Under the selected processing conditions, the cutting edge radius increases from the initial 5 μm to 14 μm and 27 μm with 4 min of treatment and 6 min of treatment preparation, respectively. The sharp cutting edge becomes rounded, the burrs and chipping on the cutting edge are eliminated, and the average roughness (Ra) of the flank face decreases from 110.4 ± 10 nm to 8.0 ± 3 nm. Nine holes were drilled consecutively by core drills after cutting edge preparation, and the cutting temperature and drilled holes’ roughness were recorded. The maximum cutting temperature (122.4 °C) in the process with the prepared core drill (radius r = 14 μm) is about 20 °C lower than that with untreated one, and the roughness of the drilled hole (Ra 510.5 nm) about 189.9 nm lower. The results indicates that FF-STP is a promising method for high consistency preparation of the core drill cutting edge

Experimental Study on Flexible Fiber Assisted Shear Thickening Polishing for Cutting Edge Preparation of Core Drill

To improve the cutting performance of the core drill, the flexible fiber assisted shear-thickening polishing (FF-STP) for cutting edge preparation was proposed to eliminate the microscopic defect and obtain proper radius of the cutting edge of the core drill. The flexible fiber was introduced into the shear-thickening polishing process to break the thickened agglomerates and improve the efficiency of cutting edge preparation. The influence of the polishing speed, abrasive concentration and the flexible fiber contact length with the core drill on the cutting edge radius r and surface morphology of the core drill edge was analyzed, and the drilling experiments were carried out after preparation, the cutting heat and drilled holes’ roughness were employed as evaluation indexes to evaluate the performance of the core drill. The results show that the cutting edge radius increases with the increase of polishing speed, abrasive concentration and contact length. However, too high a polishing speed and contact length reduce the abrasive particles involved in the polishing process, and then lead to a decline in preparation efficiency. Under the selected processing conditions, the cutting edge radius increases from the initial 5 μm to 14 μm and 27 μm with 4 min of treatment and 6 min of treatment preparation, respectively. The sharp cutting edge becomes rounded, the burrs and chipping on the cutting edge are eliminated, and the average roughness (Ra) of the flank face decreases from 110.4 ± 10 nm to 8.0 ± 3 nm. Nine holes were drilled consecutively by core drills after cutting edge preparation, and the cutting temperature and drilled holes’ roughness were recorded. The maximum cutting temperature (122.4 °C) in the process with the prepared core drill (radius r = 14 μm) is about 20 °C lower than that with untreated one, and the roughness of the drilled hole (Ra 510.5 nm) about 189.9 nm lower. The results indicates that FF-STP is a promising method for high consistency preparation of the core drill cutting edge