Shades of empire: police photography in German South-West Africa

This article looks at a photographic album produced by the German police in colonial Namibia just before World War I. Late 19th- and early 20th-century police photography has often been interpreted as a form of visual production that epitomized power and regimes of surveillance imposed by the state apparatuses on the poor, the criminal and the Other. On the other hand police and prison institutions became favored sites where photography could be put at the service of the emergent sciences of the human body—physiognomy, anthropometry and anthropology. While the conjuncture of institutionalized colonial state power and the production of scientific knowledge remain important for this Namibian case study, the article explores a slightly different set of questions. Echoing recent scholarship on visuality and materiality the photographic album is treated as an archival object and visual narrative that was at the same time constituted by and constitutive of material and discursive practices within early 20th-century police and prison institutions in the German colony. By shifting attention away from image content and visual codification alone toward the question of visual practice the article traces the ways in which the photo album, with its ambivalent, unstable and uncontained narrative, became historically active and meaningful. Therein the photographs were less informed by an abstract theory of anthropological and racial classification but rather entrenched with historically contingent processes of colonial state constitution, socioeconomic and racial stratification, and the institutional integration of photography as a medium and a technology into colonial policing. The photo album provides a textured sense of how fragmented and contested these processes remained throughout the German colonial period, but also how photography could offer a means of transcending the limits and frailties brought by the realities on the ground.International Bibliography of Social Science

German Colonialism and the British Neighbour in Africa before 1914: Self-definitions, Lines of demarcation and Co-operation

Lindner U. German Colonialism and the British Neighbour in Africa before 1914: Self-definitions, Lines of demarcation and Co-operation. In: Langbehn V, Salamaa M, eds. Colonial (Dis)-Continuities: Race, Holocaust, and Postwar Germany,. New York: Columbia University Press; 2011: 254-272

Plasmodium berghei MAPK1 displays differential and dynamic subcellular localizations during liver stage development

Mitogen-activated protein kinases (MAPKs) regulate key signaling events in eukaryotic cells. In the genomes of protozoan Plasmodium parasites, the causative agents of malaria, two genes encoding kinases with significant homology to other eukaryotic MAPKs have been identified (mapk1, mapk2). In this work, we show that both genes are transcribed during Plasmodium berghei liver stage development, and analyze expression and subcellular localization of the PbMAPK1 protein in liver stage parasites. Live cell imaging of transgenic parasites expressing GFP-tagged PbMAPK1 revealed a nuclear localization of PbMAPK1 in the early schizont stage mediated by nuclear localization signals in the C-terminal domain. In contrast, a distinct localization of PbMAPK1 in comma/ring-shaped structures in proximity to the parasite's nuclei and the invaginating parasite membrane was observed during the cytomere stage of parasite development as well as in immature blood stage schizonts. The PbMAPK1 localization was found to be independent of integrity of a motif putatively involved in ATP binding, integrity of the putative activation motif and the presence of a predicted coiled-coil domain in the C-terminal domain. Although PbMAPK1 knock out parasites showed normal liver stage development, the kinase may still fulfill a dual function in both schizogony and merogony of liver stage parasites regulated by its dynamic and stage-dependent subcellular localization

Expression and localization of <i>P. berghei</i> MAPK1 during liver stage development.

<p>(<b>A</b>) <b>Transcripts encoding PbMAPK1 and PbMAPK2 are detectable in </b><b><i>P. berghei</i></b><b> liver stage parasites.</b> Total RNA was prepared from <i>P. berghei</i>-infected HepG2 cells 24 hpi, 30 hpi, 48 hpi and 54 hpi. RT-PCR analysis was performed using primer pairs specific for <i>pbmapk1</i>, <i>pbmapk2</i>, and the constitutively expressed <i>pbtubulin</i>. To rule out false positive results originating from gDNA contamination, samples lacking reverse transcriptase (-RT) were processed in parallel. (<b>B</b>) <b>Subcellular localization of the endogenous PbMAPK1 protein during liver stage development.</b> HepG2 cells were infected with <i>Pb</i>^endPbMAPK1-GFP parasites in which the endogenous <i>mapk1</i> locus has been <i>gfp</i>-tagged by homologous recombination. Expression and localization of the PbMAPK1-GFP fusion protein was assayed at different time points of liver stage development (late schizont, cytomere, merozoites) by confocal live cell imaging. Parasite and host cell nuclei were visualized using Hoechst 33342. Arrowheads indicate partial co-localization of PbMAPK1 with parasite nuclei in the late schizont. Scale bars: 5 µm.</p

Schematic representation: stage-dependent subcellular localization of GFP-tagged MAPK1 in <i>P. berghei</i> liver stage parasites.

<p>While PbMAPK1 is observed inside the parasite nuclei at early schizont stage, at cytomere stage a distinct, comma/ring-shaped localization pattern occurs. PVM: parasitophorous vacuole membrane; PPM: parasite plasma membrane; PN: parasite nucleus; HCN: host cell nucleus.</p

Nuclear localization of PbMAPK1 is mediated by functional NLSs in the C-terminal domain.

<p>(<b>A</b>) Schematic representation of PbMAPK1 domain structure and position of predicted nuclear localization signals in the catalytic domain (aa 41–57; aa 285–289) and the C-terminal domain (aa 369–372; aa 436–444); aa: amino acids. (<b>B</b>) Alignment of putative nuclear localization signals NLS1 and NLS2 (red) in the PbMAPK1 C-terminal domains of rodent malaria parasites <i>P. berghei</i> (Pb), <i>P. yoelii</i> (Py) and <i>P. chabaudi</i> (Pc). (<b>C</b>) HepG2 cells were infected with either <i>Pb</i>^conPbMAPK1-catD(D178A)-GFP or <i>Pb</i>^conGFP-PbMAPK1-CTD parasites. 30 hpi host cell and parasite nuclei were stained using Hoechst 33342 and live cell imaging was performed. Scale bar: 5 µm.</p

Characterization of PbMAPK1 NLSs in HepG2 cells and <i>P. berghei</i> liver stage parasites.

<p>(<b>A</b>) Schematic representation of PbMAPK1-CTD mutants tested in HepG2 cells and summary of results (for confocal images see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059755#pone.0059755.s004" target="_blank">Figure S4</a>). HepG2 cells were transiently transfected with pEGFP-C2-based plasmids encoding the indicated GFP-PbMAPK1-CTD deletion constructs. 24 hours post transfection, nuclei were stained using Hoechst 33342 and subcellular localization of the fusion proteins was analyzed by live cell imaging; aa: amino acids; n.d.: not determined (due to very low expression levels of the respective fusion proteins). (<b>B</b>) HepG2 cells were infected with either <i>Pb</i>^conGFP-PbMAPK1-CTD-Δ1 or <i>Pb</i>^conGFP-PbMAPK1-CTD-Δ2 parasites. 32 hpi host cell and parasite nuclei were stained using Hoechst 33342 and live cell imaging was performed. Scale bar: 5 µm.</p

The developmentally regulated re-localization of PbMAPK1 from the nucleus to extra-nuclear comma/ring-shaped structures is dependent on both the catalytic and the C-terminal domain.

<p>HepG2 cells were infected with either <i>Pb</i>^conPbMAPK1-catD(D178A)-GFP or <i>Pb</i>^conGFP-PbMAPK1-CTD parasites. 54 hpi host cell and parasite nuclei were stained using Hoechst 33342 and live cell imaging was performed. Scale bar: 10 µm.</p