75 research outputs found
Ariel - Volume 7 Number 2
Editors
Mark Dembert
Frank Chervanek
John Lammie
Jim Burke
Curt Cumming
Ariel - Volume 6 Number 1
Editors
John Lammie
Curt Cummings
Frank Chervenak
J.D. Kanofsky
Mark Dembert
Entertainment
Robert Breckenridge
Joe Conti
Gary Kaskey
Photographer
Larry Glazerman
Overseas Editor
Mike Sinason
Circulation
Jay Amsterdam
Humorist
Jim McCann
Staff
Ken Jaffe
Bob Sklaroff
Halley Faus
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Enteropathogen antibody dynamics and force of infection among children in low-resource settings.
Little is known about enteropathogen seroepidemiology among children in low-resource settings. We measured serological IgG responses to eight enteropathogens (Giardia intestinalis, Cryptosporidium parvum, Entamoeba histolytica, Salmonella enterica, enterotoxigenic Escherichia coli, Vibrio cholerae, Campylobacter jejuni, norovirus) in cohorts from Haiti, Kenya, and Tanzania. We studied antibody dynamics and force of infection across pathogens and cohorts. Enteropathogens shared common seroepidemiologic features that enabled between-pathogen comparisons of transmission. Overall, exposure was intense: for most pathogens the window of primary infection was <3 years old; for highest transmission pathogens primary infection occurred within the first year. Longitudinal profiles demonstrated significant IgG boosting and waning above seropositivity cutoffs, underscoring the value of longitudinal designs to estimate force of infection. Seroprevalence and force of infection were rank-preserving across pathogens, illustrating the measures provide similar information about transmission heterogeneity. Our findings suggest antibody response can be used to measure population-level transmission of diverse enteropathogens in serologic surveillance
Ariel - Volume 6 Number 4
Editors
Mark Dembert
J.D. Kanofsky
Frank Chervenak
John Lammie
Curt Cummings
Entertainment
Robert Breckenridge
Joe Conti
Gary Kaskey
Photographer
Larry Glazerman
Overseas Editor
Mike Sinason
Humorist
Jim McCann
Staff
Ken Jaffe
Bob Sklaroff
Halley Faust
Jim Burk
The interaction of unfolding α-lactalbumin and malate dehydrogenase with the molecular chaperone αB-crystallin: a light and X-ray scattering investigation
Purpose: The molecular chaperone αB-crystallin is found in high concentrations in the lens and is present in all major body tissues. Its structure and the mechanism by which it protects its target protein from aggregating and precipitating are not known. Methods: Dynamic light scattering and X-ray solution scattering techniques were used to investigate structural features of the αB-crystallin oligomer when complexed with target proteins under mild stress conditions, i.e., reduction of α- lactalbumin at 37 °C and malate dehydrogenase when heated at 42 °C. In this investigation, the size, shape and particle distribution of the complexes were determined in real-time following the induction of stress. Results: Overall, it is observed that the mass distribution, hydrodynamic radius, and spherical shape of the αB-crystallin oligomer do not alter significantly when it complexes with its target protein. Conclusions: The data are consistent with the target protein being located in the outer protein shell of the αB-crystallin oligomer where it is readily accessible for possible refolding via the action of other molecular chaperones. © 2010 Molecular Vision
Ariel - Volume 6 Number 4 (Alternate Version)
Editors
Mark Dembert
J.D. Kanofsky
Frank Chervenak
John Lammie
Curt Cummings
Entertainment
Robert Breckenridge
Joe Conti
Gary Kaskey
Photographer
Larry Glazerman
Overseas Editor
Mike Sinason
Humorist
Jim McCann
Staff
Kenn Jaffe
Bob Sklaroff
Halley Faust
Jim Burke
Jay Amsterdam
Morton A. Klein
Nancy Redfer
Priority use cases for antibody-detecting assays of recent malaria exposure as tools to achieve and sustain malaria elimination
Measurement of malaria specific antibody responses represents a practical and informative method for malaria control programs to assess recent exposure to infection. Technical advances in recombinant antigen production, serological screening platforms, and analytical methods have enabled the identification of several target antigens for laboratory based and point-of-contact tests. Questions remain as to how these serological assays can best be integrated into malaria surveillance activities to inform programmatic decision-making. This report synthesizes discussions from a convening at Institut Pasteur in Paris in June 2017 aimed at defining practical and informative use cases for serology applications and highlights five programmatic uses for serological assays including: documenting the absence of transmission; stratification of transmission; measuring the effect of interventions; informing a decentralized immediate response; and testing and treating P. vivax hypnozoite carriers
Feasibility and Effectiveness of Basic Lymphedema Management in Leogane, Haiti, an Area Endemic for Bancroftian Filariasis
Lymphatic filariasis is a parasitic disease that is spread by mosquitoes. In tropical countries where lymphatic filariasis occurs, approximately 14 million people suffer from chronic swelling of the leg, known as lymphedema. Repeated episodes of bacterial skin infection (acute attacks) cause lymphedema to progress to its disfiguring form, elephantiasis. To help achieve the goal of eliminating lymphatic filariasis globally, the World Health Organization recommends basic lymphedema management, which emphasizes hygiene, skin care, exercise, and leg elevation. Its effectiveness in reducing acute attack frequency, as well as the role of compressive bandaging, have not been adequately evaluated in filariasis-endemic areas. Between 1995 and 1998, we studied 175 people with lymphedema of the leg in Leogane, Haiti. During Phase I of the study, when compression bandaging was used to reduce leg volume, the average acute attack rate was 1.56 episodes per year; it was greater in people who were illiterate and those who used compression bandages. After March 1997, when hygiene and skin care were emphasized and bandaging discouraged, acute attack frequency significantly decreased to 0.48 episodes per year. This study highlights the effectiveness of hygiene and skin care, as well as limitations of compressive bandaging, in managing lymphedema in filariasis-endemic areas
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