Comparative analysis of the proteome of the phytopathogen Rhodococcus fascians grown in presence and in absence of a tobacco leafy gall extract

info:eu-repo/semantics/nonPublishe

Proteomic analyis of the phytopathogen Rhodococcus fascians grown in conditions inducing the expression of its virulence genes

info:eu-repo/semantics/nonPublishe

Patterns of Failure in Patients With Head and Neck Squamous Cell Carcinomas of Unknown Primary Treated With Chemoradiotherapy

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Metabolic shift in the phytopathogen Rhodococcus fascians in response to cell-free extract of infected tobacco plant tissues.

The phytopathogen Rhodococcus fascians induces the development of leafy gall, which is considered to be its ecological niche. To obtain a view of the metabolic changes occurring in R. fascians during this process, an in vitro system was used where bacteria are grown in the presence of a leafy gall extract, a condition mimicking that found by the bacteria in infected plants. Proteins of R. fascians grown for 24 h under these conditions were displayed by two-dimensional polyacrylamide gel electrophoresis. Fifteen polypeptides showing a differential accumulation in response to the inducing conditions were analyzed by mass spectrometry. Two polypeptides potentially linked to the Krebs cycle, a pyruvate dehydrogenase and a fumarate hydratase, were further characterized and shown to be downregulated at the transcriptional level. The identification of these two enzymes suggests that R. fascians may shift its metabolism during the interaction with plants from the Krebs cycle to the glyoxylate shunt.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe

Genetic causes of macrozoospermia and proposal for an optimized genetic diagnosis strategy based on sperm parameters

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