Precision LCVD System Design with Real Time Process Control

A Laser Chemical Vapor Deposition (LCVD) system was designed using a fixed 100 Watt C02 laser focused on a moveable substrate. Temperature and height measurement devices monitor the reaction at the point of deposition to provide feedback for controlling the process. The LCVD system will use rapid prototyping technology to directly fabricate fully threedimensional ceramic, metallic, and composite parts of arbitrary shape. Potential applications include high temperature structures, electronic/photonic devices, and orthopaedic implants.Mechanical Engineerin

TUT7 catalyzes the uridylation of the 3′ end for rapid degradation of histone mRNA

The replication-dependent histone mRNAs end in a stem–loop instead of the poly(A) tail present at the 3′ end of all other cellular mRNAs. Following processing, the 3′ end of histone mRNAs is trimmed to 3 nucleotides (nt) after the stem–loop, and this length is maintained by addition of nontemplated uridines if the mRNA is further trimmed by 3′hExo. These mRNAs are tightly cell-cycle regulated, and a critical regulatory step is rapid degradation of the histone mRNAs when DNA replication is inhibited. An initial step in histone mRNA degradation is digestion 2–4 nt into the stem by 3′hExo and uridylation of this intermediate. The mRNA is then subsequently degraded by the exosome, with stalled intermediates being uridylated. The enzyme(s) responsible for oligouridylation of histone mRNAs have not been definitively identified. Using high-throughput sequencing of histone mRNAs and degradation intermediates, we find that knockdown of TUT7 reduces both the uridylation at the 3′ end as well as uridylation of the major degradation intermediate in the stem. In contrast, knockdown of TUT4 did not alter the uridylation pattern at the 3′ end and had a small effect on uridylation in the stem–loop during histone mRNA degradation. Knockdown of 3′hExo also altered the uridylation of histone mRNAs, suggesting that TUT7 and 3′hExo function together in trimming and uridylating histone mRNAs

Intrusive History and Petrogenesis of the Ash Mountain Complex, Sierra Nevada Batholith, California (USA)

The Ash Mountain Complex (AMC) in the western Sierra Nevada batholith (SNB; California, USA) is an exposure of six compositionally diverse intrusive lithologies with clear crosscutting relationships that permit a focused investigation of magma source characteristics and the relative roles of petrogenetic processes on the evolution of the system. We use new field observations, zircon U-Pb dates, major and trace element data, and Sr-Nd-Pb isotopic data to develop a model that can be applied to similar SNB intrusive suites. Stage 1 units, emplaced ca. 105 Ma, consist of two gabbros, a gabbrodiorite, and a granite. Stage 2 and stage 3 units were emplaced ca. 104 Ma and ca. 103 Ma, respectively, and are granites. We suggest that stage 1 gabbroids were derived by partial melting of lithospheric mantle, whereas coeval felsic magmas were derived by partial melting of a mafic, juvenile crustal source. Stage 2 and stage 3 granitoids were derived from similar sources that generated stage 1 granitoids, but there was greater input from evolved crust. Fractionation and/or assimilation played only a minor role in system evolution. Past studies of SNB magmas have come to conflicting conclusions about the petrogenesis of intermediate magmas that dominate the batholith; we hypothesize that mafic and felsic end members of the AMC could represent end members in mixing processes that generate these magmas. The timing of emplacement of the AMC coincides with a transition of magmatic style in the SNB, from smaller volume magmatic suites with mixed mantle and crustal sources to larger volume magmatic suites derived from greater proportions of crust

Fabrication of Advanced Thermionic Emitters Using Laser Chemical Vapor Deposition-Rapid Prototyping 498

Laser Chemical Vapor Deposition-Rapid Prototyping (LCVD-RP) is a relatively new manufacturing process. Its capabilities are ideally suited for the manufacturing of a type of electron emitter called an integrated-grid thermionic emitter. The integrated-grid thermionic emitter is composed of wagon wheel-like structures of alternating layers of boron nitride and molybdenum on tungsten. The goal of this paper is to determine the feasibility of using LCVDRP technology to manufacture advanced thermionic emitters.Mechanical Engineerin

Heat Transfer Analysis of a Gas-Jet Laser Chemical Vapor Deposition (LCVD) Process 461

This paper describes the development of a computer model used to characterize the heat transfer properties of a gas-jet LCVD process. A commercial software package was used to combine heat transfer finite element analysis with the capabilities of computational fluid dynamic software (CFDS). Such a model is able to account for both conduction and forced convection modes of heat transfer. The maximum substrate temperature was studied as a function of laser power and gas-jet velocity.Mechanical Engineerin

The Neurospora crassa TOB Complex: Analysis of the Topology and Function of Tob38 and Tob37

The TOB or SAM complex is responsible for assembling several proteins into the mitochondrial outer membrane, including all β-barrel proteins. We have identified several forms of the complex in Neurospora crassa. One form contains Tob55, Tob38, and Tob37; another contains these three subunits plus the Mdm10 protein; while additional complexes contain only Tob55. As previously shown for Tob55, both Tob37 and Tob38 are essential for viability of the organism. Mitochondria deficient in Tob37 or Tob38 have reduced ability to assemble β-barrel proteins. The function of two hydrophobic domains in the C-terminal region of the Tob37 protein was investigated. Mutant Tob37 proteins lacking either or both of these regions are able to restore viability to cells lacking the protein. One of the domains was found to anchor the protein to the outer mitochondrial membrane but was not necessary for targeting or association of the protein with mitochondria. Examination of the import properties of mitochondria containing Tob37 with deletions of the hydrophobic domains reveals that the topology of Tob37 may be important for interactions between specific classes of β-barrel precursors and the TOB complex