Taller de introducción a la ética y legislación en experimentación animal

Memoria ID-092. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2019-2020.[ES]Taller de ética para impulsar la adquisición de competencias ligadas a la experimentación animal. La actividad, de carácter presencial, consiste en un seminario seguido de una sesión de preguntas y discusión, y una visita formativa guiada al Animalario OMG de la Universidad de Salamanc

Empleo de la plataforma multimedia Kahoot! para fomentar el aprendizaje interactivo en las clases de teoría y seminarios de Genética

Memoria ID-0093. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2017-2018

Aprendizaje de estrategias de investigación científica en temas de biología fundamental y biomedicina

Memoria ID-0178. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2017-2018

Caracterización bioquímica y molecular del sistema de dos componentes TODS/TODT de Pseudomonas putida DOT-T1E

Capítulo 3: Resultados, escrito íntegramente en inglésTesis Univ. Granada. Departamento de Bioquímica y Biología Molecular I. Leída el 8 de abril de 2008Tesis en colaboración con el Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidí

Proteomic and Transcriptomic Profiling Identifies Early Developmentally Regulated Proteins in Dictyostelium Discoideum

© 2019 by the authors.Cyclic AMP acts as a secondary messenger involving different cellular functions in eukaryotes. Here, proteomic and transcriptomic profiling has been combined to identify novel early developmentally regulated proteins in eukaryote cells. These proteomic and transcriptomic experiments were performed in Dictyostelium discoideum given the unique advantages that this organism offers as a eukaryotic model for cell motility and as a nonmammalian model of human disease. By comparing whole-cell proteome analysis of developed (cAMP-pulsed) wild-type AX2 cells and an independent transcriptomic analysis of developed wild-type AX4 cells, our results show that up to 70% of the identified proteins overlap in the two independent studies. Among them, we have found 26 proteins previously related to cAMP signaling and identified 110 novel proteins involved in calcium signaling, adhesion, actin cytoskeleton, the ubiquitin-proteasome pathway, metabolism, and proteins that previously lacked any annotation. Our study validates previous findings, mostly for the canonical cAMP-pathway, and also generates further insight into the complexity of the transcriptomic changes during early development. This article also compares proteomic data between parental and cells lacking glkA, a GSK-3 kinase implicated in substrate adhesion and chemotaxis in Dictyostelium. This analysis reveals a set of proteins that show differences in expression in the two strains as well as overlapping protein level changes independent of GlkA.This research was funded by CSIC and Junta de Castilla y León, project number EDU/574/2018, and partially supported by FEDE

Bioavailability of pollutants and chemotaxis

The exposure of bacteria to pollutants induces frequently chemoattraction or chemorepellent reactions. Recent research suggests that the capacity to degrade a toxic compound has co-evolved in some bacteria with the capacity to chemotactically react to it. There is an increasing amount of data which show that chemoattraction to biodegradable pollutants increases their bioavailability which translates into an enhancement of the biodegradation rate. Pollutant chemoreceptors so far identified are encoded on degradation or resistance plasmids. Genetic engineering of bacteria, such as the transfer of chemoreceptor genes, offers thus the possibility to optimize biodegradation processes.During the preparation of the manuscript, our research laboratories at EEZ- CSIC and IRNAS-CSIC were supported by the Spanish Ministry of Economy and Competitiveness (grants Bio2010-16937 and CGL2010- 22068-C02-01), Junta de Andalucı ́ a (P09-RNM-4509 and RNM-312) and the BBVA Foundation (BIOCON08 185/09Peer Reviewe

Exam and assessment of contents of social sciences in 5th and 6th grade of elementary school. Capacities, concepts, and procedures

The objective of this essay is to analyze the exams of 5th and 6th grade of Elementary School about social sciences. In this paper we will analyze the contents of history and geography and the mental operations necessary to respond to the questions of the exams. This investigation has been realised on 69 exams and 745 questions picked up in five schools of the Region of Murcia. The study of these instruments of assessment has allowed us to establish different categories between the capacities, the contents and the format of examination

Bacterial sensor kinase TodS interacts with agonistic and antagonistic signals

The TodS/TodT two-component system controls expression of the toluene dioxygenase (TOD) pathway for the metabolism of toluene in Pseudomonas putida DOT-T1E. TodS is a sensor kinase that ultimately controls tod gene expression through its cognate response regulator, TodT. We used isothermal titration calorimetry to study the binding of different compounds to TodS and related these findings to their capacity to induce gene expression in vivo. Agonistic compounds bound to TodS and induced gene expression in vivo. Toluene was a powerful agonist, but ortho-substitutions of toluene reduced or abolished in vivo responses, although TodS recognized o-xylene with high affinity. These compounds were called antagonists. We show that agonists and antagonists compete for binding to TodS both in vitro and in vivo. The failure of antagonists to induce gene expression in vivo correlated with their inability to stimulate TodS autophosphorylation in vitro. We propose intramolecular TodS signal transmission, not molecular recognition of compounds by TodS, to be the phenomenon that determines whether a given compound will lead to activation of expression of the tod genes. Molecular modeling identified residues F46, I74, F79, and I114 as being potentially involved in the binding of effector molecules. Alanine substitution mutants of these residues reduced affinities (2- to 345-fold) for both agonistic and antagonistic compounds. Our data indicate that determining the inhibitory activity of antagonists is a potentially fruitful alternative to design specific two-component system inhibitors for the development of new drugs to inhibit processes regulated by two-component systems