Calcium Dependent CAMTA1 in Adult Stem Cell Commitment to a Myocardial Lineage

The phenotype of somatic cells has recently been found to be reversible. Direct reprogramming of one cell type into another has been achieved with transduction and over expression of exogenous defined transcription factors emphasizing their role in specifying cell fate. To discover early and novel endogenous transcription factors that may have a role in adult-derived stem cell acquisition of a cardiomyocyte phenotype, mesenchymal stem cells from human and mouse bone marrow and rat liver were co-cultured with neonatal cardiomyocytes as an in vitro cardiogenic microenvironment. Cell-cell communications develop between the two cell types as early as 24 hrs in co-culture and are required for elaboration of a myocardial phenotype in the stem cells 8-16 days later. These intercellular communications are associated with novel Ca(2+) oscillations in the stem cells that are synchronous with the Ca(2+) transients in adjacent cardiomyocytes and are detected in the stem cells as early as 24-48 hrs in co-culture. Early and significant up-regulation of Ca(2+)-dependent effectors, CAMTA1 and RCAN1 ensues before a myocardial program is activated. CAMTA1 loss-of-function minimizes the activation of the cardiac gene program in the stem cells. While the expression of RCAN1 suggests involvement of the well-characterized calcineurin-NFAT pathway as a response to a Ca(2+) signal, the CAMTA1 up-regulated expression as a response to such a signal in the stem cells was unknown. Cell-cell communications between the stem cells and adjacent cardiomyocytes induce Ca(2+) signals that activate a myocardial gene program in the stem cells via a novel and early Ca(2+)-dependent intermediate, up-regulation of CAMTA1

Multi-criteria evaluation used as a tool taking into account the optimal selection of materials, described on example of selection of thermal insulation materials for wood-based envelope

Currently, the choice of materials for the construction of buildings should take into account not only their traditional features such as price and quality but also the environmental qualities. The thesis describes the process of multi-criteria evaluation of thermal insulation materials for wood-basednbspenvelope with other possible comparing of obtained valuation parameters.nbs

Mesenchymal Stem Cells as Regulators of the Bone Marrow and Bone Components

Even though bone marrow mesenchymal stem cells and the “endosteal and vascular hematopoietic niche” have been an active area of study, the concept of the bone marrow/bone microenvironment harboring a niche for solid metastatic tumor cells was only recently considered. The hematopoietic stem cell niche that in some cases is thought to constitute the tumor niche shares many of the same structural and functional components, suggesting the possibility that the “hematopoietic and tumor niches” are one in the same. Mesenchymal stem cells, as well as the osteoblasts, are critical components for each of these niches and are important components for regulating cellular processes such as homing and migration, proliferation and survival, and quiescence and dormancy of tumor cells. Current understanding of mesenchymal stem cell properties may provide more insight to better defining the solid tumor niche, especially in the development of the bone marrow and bone “premetastatic niche.”Fil: Martinez, Leandro Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Labovsky, Vivian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Fernández Vallone, V. B.. Université Libre de Bruxelles; BélgicaFil: Choi, H.. Central Texas Veterans Research Foundation; Estados UnidosFil: Phillips, C.. Central Texas Veterans Research Foundation; Estados UnidosFil: Amorós, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentin

Emulsion electrospun nanofibers as substrates for cardiomyogenic differentiation of mesenchymal stem cells

10.1007/s10856-013-5003-5Journal of Materials Science: Materials in Medicine24112577-2587JSMM