Meristema apical como ferramenta de manejo da pastagem para pastos de capim Tanzânia (Panicum maximum cv. Tanzânia).

Determinar as relações entre altura do pasto e altura do meristema apical em pasto de capim-Tanzânia submetido a diferentes intensidades de manejo baseados em somas térmica

Qualidade da carcaça de ovinos terminados em confinamento com níveis de bagana de carnaúba na dieta.

Objetivou-se avaliar os efeitos da utilização da bagana de carnaúba (Copernicia prunifera (Mill.) H.E. Moore) (BC), como fonte de volumoso, sobre as características da carcaça de cordeiros terminados em confinamento. Foram utilizados 30 cordeiros mestiços Santa Inês x SRD desmamados, com peso corporal médio de 16,9 + 1,56 kg e idade média de 80 dias. Foram formuladas dietas com cinco níveis de substituição do feno de capim-tyfton 85 (Cynodon spp.) (FT) pela BC: 0%BC e 100%FT (testemunha); 25%BC e 75%FT; 50%BC e 50%FT; 75%BC e 25%FT; 100%BC e 0%FT. Os teores de umidade, proteína e cinzas da carne não foram influenciados pelas dietas. O peso ao abate, peso de carcaça quente, peso de carcaça fria e os rendimentos de carcaça quente e de carcaça fria diminuíram à medida que o FT era substituído pela BC. Os itens quebra ao resfriamento e perda ao jejum não foram influenciados pelas dietas. Os valores mais elevados para comprimentos de carcaça e de pernil, peso de pernil, peso de paleta e pesos de pernil, de paleta e de lombo foram observados no tratamento testemunha, enquanto os valores mais baixos foram observados no tratamento que recebeu apenas BC. Verificou-se o efeito das dietas sobre as porcentagens de tecido ósseo no pernil e na paleta e sobre as porcentagens de tecido ósseo, conectivo e adiposo do lombo. Conclui-se que a inclusão da bagana de carnaúba na dieta interfere negativamente no consumo e, consequentemente, nas características qualitativas da carcaça de cordeiros em terminação

Características quantitativas da carcaça de ovinos alimentados com dietas compostas por silagens com diferentes proporções de sorgo e girassol.

O objetivo deste experimento foi estudar características quantitativas da carcaça de ovinos terminados em confinamento, recebendo dietas compostas por silagens com diferentes proporções de sorgo e girassol. As dietas experimentais foram constituídas por 60% de volumoso e 40% de concentrado. Foram utilizados 30 cordeiros mestiços Santa Inês, machos não castrados, com média de peso inicial de 19,51 kg. Os animais foram abatidos após 56 dias de confinamento em baias individuais (4,0m2), alimentados com dietas contendo 0; 25; 50; 75 e 100% de silagem de girassol em substituição ao sorgo no volumoso. Não houve efeito significativo para as características de peso vivo ao abate (PVA), peso de corpo vazio (PCV), peso de carcaça quente (PCQ) e peso de carcaça fria (PCF), para os animais que receberam até 75% de silagem de girassol. As médias para as variáveis PVA, PCV, PCQ e PCF foram 33,59; 29,95; 16,14 e 14,83 kg, respectivamente. Os valores obtidos em relação aos rendimentos de carcaça quente e fria, não foram significativos para as dietas com até 100% de silagem de girassol, apresentando valores médios de 48,03 e 44,13%, respectivamente. O rendimento biológico médio foi de 54,24%. A silagem de girassol pode substituir a de sorgo em até 75% do volumoso, sem afetar de maneira significativa, as características quantitativas da carcaça de ovinos terminados em confinamento. Carcass quantitative characteristics of sheeps receiving diets containing different levels of sunflower silage in substitution of sorghum silage Abstract: The objective of this experiment was to study quantitative carcass traits of feedlot lambs fed diets with silages consisted of different proportions of sorghum and sunflower. The experimental diets consisted of 60% roughage and 40% concentrate. A total of 30 male lambs crossbred Santa Inês, non castrated, with initial average weight of 19.51 kg were evaluated. The animals were slaughtered after 56 days in feedlot in individual stalls (4,0m2), fed diets with 0; 25; 50; 75 and 100% of sunflower participation in silage mixed with sorghum. There was not an effect on the characteristics of live weight at slaughter (LWS), empty body weight (EBW), hot carcass weight (HCW) and cold carcass weight (CCW) for the animals that received silage with up to 75% of sunflower participation. The averages for the variables LWS, PCV, HCW and CCW were 33.59; 29.95; 16.14 and 14.83 kg, respectively. The values obtained for the hot and chilled yield were not significant for silages with up to 100% sunflower participation, with average values of 48.03 and 44.13% respectively. The biological average yield was 54.24%. Sunflower can substitute sorghum on silage composition up to 75%, without significantly affect the quantitative characteristics of feedlot lambs carcass

Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia

DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy of species-level assignment, and consequent taxonomic coverage, relies on comprehensive DNA barcode reference libraries, which, due to incompleteness, are currently a recognized limitation for metabarcoding applications. In this study, we assembled a comprehensive reference library of DNA barcodes for Atlantic Iberia marine macroinvertebrate species, assessed gaps in species coverage and examined data ambiguities. Initially, an Iberian species checklist for the three dominant groups of marine macroinvertebrates was compiled, comprising 2827 species (926 Annelida, 638 Crustacea and 1263 Mollusca). A total of 18162 DNA sequences of the cytochrome c oxidase subunit I barcode region (COI-5P) matching the species checklist were compiled in a BOLD dataset, where taxonomic discordances were evaluated and cases of deep intraspecific divergence flagged. Gap-analysis showed that 63% of the Iberian macroinvertebrate species still lack a DNA barcode. Coverage gaps varied considerably across taxonomic groups with Mollusca displaying the highest sequence representation in the dataset (427 species, 49% of the total number of sequences), and Crustacea the highest species coverage with 338 species barcoded (53% of the checklist). In contrast, Polychaeta displayed the lower levels of completion (288 species, 16% of the total number of sequences). In total, 1545 Barcode Index Numbers (BINs) were assigned to 1053 barcoded species, of which 66% were taxonomically concordant, 26% displayed multiple BINs and 8% were discordant. Overall, results show that there is still a large portion of marine invertebrate taxa in this region of Europe pending barcode coverage, even considering only the dominant groups. However, the most notable finding was the relevant proportion of species flagged for significant intraspecific divergence and possible hidden diversity. The annotated reference library and gap-analysis here provided can therefore contribute to prioritize marine macroinvertebrate taxa for future research efforts and barcode coverage.Fundação para a Ciência e a Tecnologia | Ref. UIDB/04050/2020Fundação para a Ciência e a Tecnologia | Ref. PD/BD/127994/2016Fundação para a Ciência e a Tecnologia | Ref. SFRH/BD/131527/201

Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia

DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy of species-level assignment, and consequent taxonomic coverage, relies on comprehensive DNA barcode reference libraries, which, due to incompleteness, are currently a recognized limitation for metabarcoding applications. In this study, we assembled a comprehensive reference library of DNA barcodes for Atlantic Iberia marine macroinvertebrate species, assessed gaps in species coverage and examined data ambiguities. Initially, an Iberian species checklist for the three dominant groups of marine macroinvertebrates was compiled, comprising 2827 species (926 Annelida, 638 Crustacea and 1263 Mollusca). A total of 18162 DNA sequences of the cytochrome c oxidase subunit I barcode region (COI-5P) matching the species checklist were compiled in a BOLD dataset, where taxonomic discordances were evaluated and cases of deep intraspecific divergence flagged. Gap-analysis showed that 63% of the Iberian macroinvertebrate species still lack a DNA barcode. Coverage gaps varied considerably across taxonomic groups with Mollusca displaying the highest sequence representation in the dataset (427 species, 49% of the total number of sequences), and Crustacea the highest species coverage with 338 species barcoded (53% of the checklist). In contrast, Polychaeta displayed the lower levels of completion (288 species, 16% of the total number of sequences). In total, 1545 Barcode Index Numbers (BINs) were assigned to 1053 barcoded species, of which 66% were taxonomically concordant, 26% displayed multiple BINs and 8% were discordant. Overall, results show that there is still a large portion of marine invertebrate taxa in this region of Europe pending barcode coverage, even considering only the dominant groups. However, the most notable finding was the relevant proportion of species flagged for significant intraspecific divergence and possible hidden diversity. The annotated reference library and gap-analysis here provided can thereThis study was supported by the project The NextSea [NORTE-01-0145-FEDER-000032], under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work was supported by the "Contrato-Programa'' UIDB/04050/2020 funded by national funds through the FCT I.P. SD and PEV work was supported by national funds through the Portuguese Foundation for Science and Technology (FCT, I.P.) in the scope of the project NIS-DNA [PTDC/BIA-BMA/29754/2017]. BRL benefitted from an FCT fellowship PD/BD/127994/2016. MALT benefitted from an FCT fellowship co-financed by European Social Fund (ESF) SFRH/BD/131527/2017

Proposta de mudanças no regulamento do registro genealógico das raças zebuínas.

Apresenta-se neste trabalho um breve histórico dos registros genealógicos, com referencia a sua importância para o melhoramento genético animal. Ênfase especial e dada para o caso das raças zebuínas, criadas no Brasil. Em decorrência do fechamento do livro de registro; ocorrido em 1971 para a maioria das raças, e, em função de problemas conjunturais ocorridos desde então, e grande, atualmente, o contingente de animais que se encontra a margem do controle oficial. Por outro lado, os rebanhos considerados puros não conseguem suprir sequer a metade da demanda anual de reprodutores, necessária para a reposição nos planteis de seleção e nos rebanhos comerciais. Com base na analise e discussão de aspectos práticos, genéticos, econômicos e políticos, apresenta-se uma proposta de mudança no atual regulamento, de forma a proporcionar um melhor aproveitamento dos recursos genéticos disponíveis. Dentre vários benefícios da nova metodologia de registros salientam-se: simplificação dos processos de colheitas, armazenamento, tramitação e analise de dados e de documentos, aumento da variabilidade genética, redução da endogamia, aumento da intensidade de seleção e estimulo para o crescimento de agrupamentos raciais menores, em risco de extinção.bitstream/item/104802/1/Proposta-de-mudanca-no-regulamento.pd

development of an occupational health risk management program

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Horava-Lifshitz Holography

We derive the detailed balance condition as a solution to the Hamilton-Jacobi equation in the Horava-Lifshitz gravity. This result leads us to propose the existence of the d-dimensional quantum field theory on the future boundary of the (d+1)-dimensional Horava-Lifshitz gravity from the viewpoint of the holographic renormalization group. We also obtain a Ricci flow equation of the boundary theory as the holographic RG flow, which is the Hamilton equation in the bulk gravity, by tuning parameters in the theory.Comment: 7 page

Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons

© The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Cutaneous secretions of amphibians have bioactive compounds, such as peptides, with potential for biotechnological applications. Therefore, this study aimed to determine the primary structure and investigate peptides obtained from the cutaneous secretions of the amphibian, Leptodactylus vastus, as a source of bioactive molecules. The peptides obtained possessed the amino acid sequences, GVVDILKGAAKDLAGH and GVVDILKGAAKDLAGHLASKV, with monoisotopic masses of [M + H]± = 1563.8 Da and [M + H]± = 2062.4 Da, respectively. The molecules were characterized as peptides of the class of ocellatins and were named as Ocellatin-K1(1-16) and Ocellatin-K1(1-21). Functional analysis revealed that Ocellatin-K1(1-16) and Ocellatin-K1(1-21) showed weak antibacterial activity. However, treatment of mice with these ocellatins reduced the nitrite and malondialdehyde content. Moreover, superoxide dismutase enzymatic activity and glutathione concentration were increased in the hippocampus of mice. In addition, Ocellatin-K1(1-16) and Ocellatin-K1(1-21) were effective in impairing lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) formation and NF-kB activation in living microglia. We incubated hippocampal neurons with microglial conditioned media treated with LPS and LPS in the presence of Ocellatin-K1(1-16) and Ocellatin-K1(1-21) and observed that both peptides reduced the oxidative stress in hippocampal neurons. Furthermore, these ocellatins demonstrated low cytotoxicity towards erythrocytes. These functional properties suggest possible to neuromodulatory therapeutic applications.Alexandra Plácido is a recipient of a post-doctoral grant from the project FCT (PTDC/BII-BIO/31158/2017). Renato Socodato and Camila Cabral Portugal hold postdoctoral fellowships from FCT (Refs: SFRH/BPD/91833/2012 and FRH/BPD/91962/2012, respectively). This work was funded through project UID/QUI/50006/2013-POCI/01/0145/FEDER/007265 (LAQV/REQUIMTE) with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT 2020info:eu-repo/semantics/publishedVersio