Problemas atuais da juticultura amazônica.

No presente trabalho, os autores procuraram expor os problemas atuais que entravam o desenvolvimento da juticultura amazônica, ressaltando a importância marcante da produção das fibras desta tiliácea como fonte da manutenção do mercado interno brasileiro de matéria prima necessária ao abastecimento do parque nacional de aniagem. Os problemas considerados, no trabalho em questão, foram: 1) problemas de ordem agrícola, 2) problemas de ordem sócio-econômica. Procurou-se, também, de forma sintética, expor a situação do mercado nacional e as possibilidades de exportação. Como conclusão, os autores admitem a necessidade urgente e prioritária de se estimular econômicamente o juticultor a fim de que se possa posteriormente mostrar que a quantidade e qualidade do produto depende em grande parte dos problemas culturais e tecnológicos.Separata da Pesquisa Agropecuária Brasileira, v. 1, p. 1-6, 1966

A juta na Amazônia.

O cultivo da juta na Amazônia. Conclusões dos trabalhos de pesquisa realizados pelo Instituto Agronômico do Norte

Influência do esterco de curral e da calagem na produção de feijão Vigna (Cow-pea) em latosolo amarelo, da região de Belém.

bitstream/item/143169/1/CIRCULAR-9-ABRIL-1964.pdfTrabalho apresentado no Congresso de Agricultura Tropical, organizado pela Estação Experimental de Paramaribo (Guiana Holandesa)

Optimization of organotypic cultures of mouse spleen for staining and functional assays

By preserving cell viability and three-dimensional localization, organotypic culture stands out among the newest frontiers of cell culture. It has been successfully employed for the study of diseases among which neoplasias, where tumoral cells take advantage of the surrounding stroma to promote their own proliferation and survival. Organotypic culture acquires major importance in the context of the immune system, whose cells cross-talk in a complex and dynamic fashion to elicit productive responses. However, organotypic culture has been as yet poorly developed for and applied to primary and secondary lymphoid organs. Here we describe in detail the development of a protocol suitable for the efficient cutting of mouse spleen, which overcomes technical difficulties related to the peculiar organ texture, and for optimized organotypic culture of spleen slices. Moreover, we used microscopy, immunofluorescence, flow cytometry and qRT-PCR to demonstrate that the majority of cells residing in spleen slices remain alive and maintain their original location in the organ architecture for several days after cutting. The development of this protocol represents a significant technical improvement in the study of the lymphoid microenvironment in both physiological and pathological conditions involving the immune system

Cultura da juta.

Publicação não convencional. Datilografado

Diastolic time – frequency relation in the stress echo lab: filling timing and flow at different heart rates

A cutaneous force-frequency relation recording system based on first heart sound amplitude vibrations has been recently validated. Second heart sound can be simultaneously recorded in order to quantify both systole and diastole duration

NMR Studies on Structure and Dynamics of the Monomeric Derivative of BS-RNase: New Insights for 3D Domain Swapping

Three-dimensional domain swapping is a common phenomenon in pancreatic-like ribonucleases. In the aggregated state, these proteins acquire new biological functions, including selective cytotoxicity against tumour cells. RNase A is able to dislocate both N- and C-termini, but usually this process requires denaturing conditions. In contrast, bovine seminal ribonuclease (BS-RNase), which is a homo-dimeric protein sharing 80% of sequence identity with RNase A, occurs natively as a mixture of swapped and unswapped isoforms. The presence of two disulfides bridging the subunits, indeed, ensures a dimeric structure also to the unswapped molecule. In vitro, the two BS-RNase isoforms interconvert under physiological conditions. Since the tendency to swap is often related to the instability of the monomeric proteins, in these paper we have analysed in detail the stability in solution of the monomeric derivative of BS-RNase (mBS) by a combination of NMR studies and Molecular Dynamics Simulations. The refinement of NMR structure and relaxation data indicate a close similarity with RNase A, without any evidence of aggregation or partial opening. The high compactness of mBS structure is confirmed also by H/D exchange, urea denaturation, and TEMPOL mapping of the protein surface. The present extensive structural and dynamic investigation of (monomeric) mBS did not show any experimental evidence that could explain the known differences in swapping between BS-RNase and RNase A. Hence, we conclude that the swapping in BS-RNase must be influenced by the distinct features of the dimers, suggesting a prominent role for the interchain disulfide bridges