Uso do modelo de crescimento 3-PG para o zoneamento do potencial produtivo do eucalipto no Estado de Minas Gerais.

O zoneamento do potencial produtivo do eucalipto para o estado de Minas Gerais, a partir das estimativas realizadas pelo modelo de crescimento 3-PG, permite a formulação d~ políticas para um melhor aproveitamento do espaço rural, direcionamento de programas de fomento florestal, seleção de áreas prioritárias para reflorestamentos e definição de níveis de produtividades locais. A parametrização do modelo levou em consideração o uso de sistemas de produção de médio e alto nível tecnológico. A partir das estimativas realizadas pelo modelo 3-PG identificou-se que o uso de alto .nível tecnológico, compreendendo o plantio de materiais selecionados e técnicas adequadas de nutrição e manejo dos povoamentos permite ganhos em produtividade cerca de duas vezes maior de. que os valores obtidos em plantios convencionais. O modelo 3-PG mostrou-se uma ferramenta adequada, para a estimativa do potencial produtivo do eucalipto, em escala regional, considerando-se as variações climáticas,' edáficas e fisiológicas. A maioria dos municípios mineiros apresenta potencial produtivo superior aos limites mínimos estabelecidos como critério de recomendação de cultivo

Brazilian vaccinia virus strains are genetically divergent and differ from the lister vaccine strain.

Vaccinia virus is responsible for an important zoonotic disease affecting dairy cattle and humans in Brazil, but little is known about the origin, epidemiology and evolution of these Brazilian Vaccinia virus strains. In this work, seven Brazilian Vaccinia virus strains and the Lister-derived Brazilian vaccine strain, named Lister-Butantan, were compared based on the sequences of ten host range and virulence related genes. Comparison of Brazilian Vaccinia virus strains with Lister-Butantan revealed several differences. Phylogenetic analyses confirmed the existence of genetically distinct Brazilian Vaccinia virus groups and has not thus far demonstrated a close relationship between Brazilian strains and Lister-Butantan. In this study, the BeAn58058 and SPAn232 strains were grouped together with the Belo Horizonte and Guarani P1 strains. Additionally, genetic polymorphisms in host range and virulence genes as well as differences in the deduced amino acid sequences were detected among Brazilian Vaccinia virus. This genetic diversity may result in a plethora of different biological properties presented by Brazilian Vaccinia virus, including differences in adaptation to the host as well as pathogenic properties. Furthermore, co-circulation of these divergent strains could increase the possibility of recombination events in nature, leading to the formation of new variants with unpredictable pathogenic potential

As variedades crioulas de cebola e o potencial de uso em sistemas agroecologicos.

Objetivou-se descrever os sistemas de cultivo de cebola tradicionais e avaliar o potencial dos genótipos crioulos para sistemas agroecológicos

Thalamic Lesions in Vascular Dementia: Low Sensitivity of Fluid-Attenuated Inversion Recovery (FLAIR) Imaging

Background and Purpose—The criteria of the National Institute of Neurological Disorders and Stroke (NINDS)– Association Internationale pour la Recherche et l’Enseignement en Neurosciences (AIREN) include thalamic lesions for the diagnosis of vascular dementia (VaD). Although studies concerning VaD and brain aging advocate the use of fluid-attenuated inversion recovery (FLAIR) or T2-weighted images (T2-WI) to detect ischemic lesions, none compared the sensitivity of these sequences to depict thalamic lesions. Methods—We performed a blinded review of T2-WI and FLAIR images in 73 patients fulfilling the radiological part of the NINDS-AIREN criteria (mean age, 71 years; range, 49 to 83 years). This sample was drawn from a large multicenter trial on VaD and was expected to have a high prevalence of thalamic lesions. In a side-by-side review, including T1-weighted images as well, lesions were classified according to presumed underlying pathology. Results—The total number of thalamic lesions was 214. Two hundred eight (97%) were detected on T2-WI, but only 117 (55%) were detected on FLAIR ( 2 5.1; P 0.05). Although the mean size of lesions detected on T2-WI and not on FLAIR (4.4 mm) was significantly lower than the mean size of lesions detected on both sequences (6.7 mm) (P 0.001), 5 of the 29 lesions 10 mm on T2-WI were not visible on FLAIR. FLAIR detected only 81 (51%) of the 158 probable ischemic lesions and 30 (60%) of the 50 probable microbleeds. Conclusions—FLAIR should not be used as the only T2-weighted sequence to detect thalamic lesions in patients suspected of having VaD

Brazilian vaccinia virus strains show genetic polymorphism at the ati gene.

Nucleotide sequence comparison of the internal region of the ati gene of members of the Orthopoxvirus genera revealed that this gene is variable among different species, although within members of the same species it is considered to be well conserved. Previous studies indicated that there is genetic variability in the ati gene among some Brazilian Vaccinia virus strains. To further investigate this variability, we performed molecular analysis of the internal region of the ati gene of eight Brazilian Vaccinia virus strains. While the internal region of this gene in one strain was similar to the Western Reserve strain, four strains presented two blocks of deletions in the analyzed region, and the ati gene was almost entirely deleted from three other strains. These findings demonstrate that there is genetic polymorphism within the ati gene among different Brazilian Vaccinia virus strains

Efeito de diferentes meios de cultura e da luz no crescimento e na esporulação de Verticillium lecanii.

Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso

Chlorophylls extraction from spinach leaves using aqueous solutions of surface-active ionic liquids

Chlorophylls and their derivatives have been extensively studied due to their unique and valuable properties, including their anti-mutagenic and anti-carcinogenic features. Nevertheless, high-purity-level chlorophylls extracted from natural sources are quite expensive because the methods used for their extraction have low selectivity and result in low yields. This study aimed to develop a “greener” and cost-effective technology for the extraction of chlorophylls from biomass using aqueous solutions of ionic liquids (ILs). Several aqueous solutions of ILs, with hydrotropic and surface-active effects were evaluated, demonstrating that aqueous solutions of surface-active ILs are enhanced solvents for the extraction of chlorophylls from spinach leaves. Operating conditions, such as the IL concentration and solid–liquid ratio, were optimized by a response surface methodology. Outstanding extraction yields (0.104 and 0.022 wt.% for chlorophyll a and b, respectively, obtained simultaneously) and selectivity (chlorophyll a/b ratio of 4.79) were obtained with aqueous solutions of hexadecylpyridinium chloride ([C16py]Cl) at moderate conditions of temperature and time. These extraction yields are similar to those obtained with pure ethanol. However, the chlorophyll a/b ratio achieved with the IL aqueous solution is higher than with pure ethanol (3.92), reinforcing the higher selectivity afforded by IL aqueous solutions as viable replacements to volatile organic compounds and allowing the obtainment of more pure compounds. Finally, the recovery and reuse of the solvent were evaluated by using a back-extraction step of chlorophylls using ethyl acetate. The results disclosed here bring new perspectives into the design of new approaches for the selective extraction of chlorophylls from biomass using aqueous solutions of surface-active ILspublishe

Dietary and nutritional patterns in an elderly rural population in Northern and Southern Italy: (II). Nutritional profiles associated with food behaviours

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Hyperendemicity, heterogeneity and spatial overlap of leprosy and cutaneous leishmaniasis in the southern Amazon region of Brazil

Neglected tropical diseases characterized by skin lesions are highly endemic in the state of Mato Grosso, Brazil. We analyzed the spatial distribution of leprosy and Cutaneous Leishmaniasis (CL) and identified the degree of overlap in their distribution. All new cases of leprosy and CL reported between 2008 and 2017 through the national reporting system were included in the study. Scan statistics together with univariate Global and Local Moran's I were employed to identify clusters and spatial autocorrelation for each disease, with the spatial correlation between leprosy and CL measured by bivariate Global and Local Moran's I. Finally, we evaluated the demographic characteristics of the patients. The number of leprosy (N = 28,204) and CL (N = 24,771) cases in Mato Grosso and the highly smoothed detection coefficients indicated hyperendemicity and spatial distribution heterogeneity. Scan statistics demonstrated overlap of high-risk clusters for leprosy (RR = 2.0; p <0.001) and CL (RR = 4.0; p <0.001) in the North and Northeast mesoregions. Global Moran's I revealed a spatial autocorrelation for leprosy (0.228; p = 0.001) and CL (0.311; p = 0.001) and a correlation between them (0.164; p = 0.001). Both diseases were found to be concentrated in urban areas among men aged 31-60 years, of brown-skinned ethnicity and with a low educational level. Our findings indicate a need for developing integrated and spatially as well as socio-demographically targeted public health policies