70 research outputs found

    Características físico-químicas e produtivas do leite de cabras de raças naturalizadas do Semiárido pernambucano.

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    Diante da importância dos caprinos para a região semiárida do estado de Pernambuco e da ausência de informações sobre as características físico-químicas do leite, o objetivo deste estudo foi realizar a caracterização físico-químicas e produtivas do leite de cabras naturalizadas e SRD que são criadas nesta região.Suplemento 1. Edição dos Anais do V Congresso Brasileiro de Qualidade do Leite do Conselho Brasileiro de Qualidade do Leite - CBQL, Águas de Lindóia, jun. 2013

    Use of Bayesian Inference to Correlate In Vitro Embryo Production and In Vivo Fertility in Zebu Bulls

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    The objective of this experiment was to test in vitro embryo production (IVP) as a tool to estimate fertility performance in zebu bulls using Bayesian inference statistics. Oocytes were matured and fertilized in vitro using sperm cells from three different Zebu bulls (V, T, and G). The three bulls presented similar results with regard to pronuclear formation and blastocyst formation rates. However, the cleavage rates were different between bulls. The estimated conception rates based on combined data of cleavage and blastocyst formation were very similar to the true conception rates observed for the same bulls after a fixed-time artificial insemination program. Moreover, even when we used cleavage rate data only or blastocyst formation data only, the estimated conception rates were still close to the true conception rates. We conclude that Bayesian inference is an effective statistical procedure to estimate in vivo bull fertility using data from IVP

    In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair

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    <p>Abstract</p> <p>Background</p> <p>Barrier materials as cellulose membranes are used for guided tissue repair. However, it is essential that the surrounding tissues accept the device. The present study histologically evaluated tissue reaction to a microbial cellulose membrane after subcutaneous implantation in mice. Furthermore, the interaction between mesenchymal stem cells and the biomaterial was studied <it>in vitro </it>to evaluate its ability to act as cellular scaffold for tissue engineering.</p> <p>Methods</p> <p>Twenty-five Swiss Albino mice were used. A 10 × 10 mm cellulose membrane obtained through biosynthesis using <it>Acetobacter xylinum </it>bacteria was implanted into the lumbar subcutaneous tissue of each mouse. The mice were euthanatized at seven, 15, 30, 60, and 90 days, and the membrane and surrounding tissues were collected and examined by histology.</p> <p>Results</p> <p>A mild inflammatory response without foreign body reaction was observed until 30 days post-surgery around the implanted membrane. Polarized microscopy revealed that the membrane remained intact at all evaluation points. Scanning electron microscopy of the cellulose membrane surface showed absence of pores. The <it>in vitro </it>evaluation of the interaction between cells and biomaterial was performed through viability staining analysis of the cells over the biomaterial, which showed that 95% of the mesenchymal stem cells aggregating to the cellulose membrane were alive and that 5% were necrotic. Scanning electron microscopy showed mesenchymal stem cells with normal morphology and attached to the cellulose membrane surface.</p> <p>Conclusion</p> <p>The microbial cellulose membrane evaluated was found to be nonresorbable, induced a mild inflammatory response and may prove useful as a scaffold for mesenchymal stem cells.</p

    Novos projetos siderúrgicos atenderão ao aumento da demanda até 2014

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    Os fascículos, ao fim de cada ano, são reunidos em uma única publicação, intitulada "Visão do Desenvolvimento

    CARACTERIZAÇÃO BIOFACIOLÓGICA DOS SEDIMENTOS DA BAÍA DE TODOS OS SANTOS, BRASIL

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    O estudo das partículas biogênicas dos sedimentos de fundo da Baía de Todos os Santos (BTS) foi realizado com o objetivo de investigar as biofácies como um instrumento de caracterização do ambiente sedimentar da baía. Através da análise de agrupamento foi possível estabelecer quatro biofácies bem definidas. Estas biofácies permitiram verificar uma transição da composição biogênica do interior da BTS onde, na maioria das vezes, os moluscos predominaram nos sedimentos, em relação à plataforma continental adjacente, onde as algas calcárias passaram a ser o componente principal. Com base nas características composicionais foi possível classificar a maior parte das assembleias biogênicas como parautóctones, ou seja, aquelas que não são transportadas para longe de seu hábitat original. Foi possível constatar que os processos tafonômicos registrados são o reflexo das condições ambientais da baía, um ambiente semi-fechado, apresentando geralmente energia baixa a moderada e uma grande diversidade de tipos de fundo

    Effects of transcutaneous electrical nerve stimulation (TENS) on arterial stiffness and blood pressure in resistant hypertensive individuals: study protocol for a randomized controlled trial

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    Background: Resistant hypertension (RH) treatment requires an adequate and intense therapeutic approach. However, the results are not always satisfactory despite intensive treatment. Of the different pathophysiological mechanisms involved in the pathogenesis of RH, sympathetic overstimulation and therapies that block the sympathetic system have been widely studied. These approaches, however, are invasive and expensive. Another possible approach is by transcutaneous electrical nerve stimulation (TENS), a noninvasive method that modulates activity by using low-frequency transcutaneous electrical stimulation to inhibit primary afferent pathways. Thus, the current study will evaluate the effect of applying TENS in the cervicothoracic region of subjects with RH and will seek to develop a new low-cost and readily available therapy to treat this group of hypertensive individuals. Methods/design: This is a randomized, single blind (subject), parallel-assignment study controlled with a sham group and including participants aged 40 to 70 years with resistant hypertension. The trial has two arms: the treatment and control (sham group). The treatment group will be submitted to the stimulation procedure (TENS). The sham group will not be submitted to stimulation. The primary outcomes will be a reduction in the peripheral blood pressure and adverse events. The secondary outcomes will be a reduction the central blood pressure. The study will last 30 days. The sample size was calculated assuming an alpha error of 5 % to reject the null hypothesis with a statistical power of 80 %, thereby resulting in 28 participants per group (intervention versus sham). Discussion: In recent decades, RH has become very common and costly. Adequate control requires several drugs, and in many cases, treatment is not successful. Sympathetic nervous system inhibition by renal denervation and central inhibition have significant effects in reducing BP; however, these treatments are costly and invasive. Another type of sympathetic nervous system inhibition can also be noninvasively achieved by electric current. Therefore, the application of TENS may be a new therapeutic option for treating resistant hypertensive individuals. Trial Registration: Clinical Trials NCT0236597

    SEDIMENTAÇÃO ATUAL DA BAÍA DE TODOS OS SANTOS COM ÊNFASE NOS COMPONENTES BIOGÊNICOS

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    O presente estudo propôs caracterizar o ambiente sedimentar atual da BTS com base na comparação dos componentes biogênicos dos sedimentos amostrados em 1974 e em 1997. Os resultados mostraram que os biogênicos identificados foram praticamente os mesmos na fração cascalho e na areia em ambas as amostragens. Entretanto, foram verificadas diferenças significativas nas médias percentuais de alguns biogênicos entre as amostras da fração areia. Os fragmentos de vegetais, por exemplo, apresentaram média percentual aproximadamente quatro vezes maior nas amostras de 1997 em relação às de 1974. Organismos produtores de sedimentos, típicos consumidores de detritos, como os ostracodes e crustáceos, também apresentaram médias percentuais maiores significativamente nas amostras de 1997. Estes resultados sugerem que a BTS parece estar passando por uma nova fase ambiental e mudanças estão sendo registradas nos seus sedimentos
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