Distribution of Kell phenotype among pregnant women in Sokoto, North Western Nigeria

Introduction: Kell antigen is highly immunogenic and is the common cause of antibody production in mismatched blood transfusions, haemolytic transfusion reaction (HTR) and maternal alloimmunization, which causes severe anaemia in neonates. The aim of this study is to determine the prevalence and ethnic variation of the Kell phenotype among pregnant women in Sokoto, Nigeria. Methods: Kell antigen status of 150 pregnant women aged 18-45 years and mean age 27.19 ±4.69 years attending antenatal clinic in UDUTH Sokoto Nigeria was determined using the conventional tube method and anti-Kell reagents (Lorne Laboratories, UK). Results: Among the 150 subjects studied, 3 (2.0%) of subjects were positive and 147 (98.0) were negative for K antigen. Of the 150 pregnant subjects; 32 (21.3%) were primigravidae while 118 (78.7%) were multigravidae. Kell phenotype was more prevalent among primigravidae (3.1%) compared to multigravidae (1.7%) women. The distribution of Kell phenotype among the pregnant subjects was compared based on ethnicity. The prevalence of Kell antigen was significantly higher among the Hausa ethnic group (3.2%) compared to other ethnic groups which indicated zero prevalence (p=0.001). Kell negative phenotype was ≥ 96.8% among all the ethnic groups. Conclusion: Our observed prevalence of Kell phenotype is consistent with previous studies among Blacks and Asians but significantly lower than values observed in previous studies among Caucasians. We recommend that all pregnant women should be screened for the presence clinically significant red cell antigens including Kell antigen on their first antenatal visit. Kell negative red cell should be routinely provided for all pregnant women and women with child bearing potential to reduce the risk of Kell-associated HDFN. There is need to introduce routine screening of pregnant women for clinically significant red cell antibodies to facilitate the effective management of HDFN as well as prevent HTR. There is also need for sustained health education of pregnant women in the area to encourage early booking for antenatal care

The Role of Renewable Energy in Mitigating Deforestation and Climate Change in Nigeria

This paper provides an overview of the role of renewable energy in mitigating deforestation and climate change in Nigeria. There are strong concerns over greenhouse gas (GHGs) buildup in the atmosphere which is leading to global climate change. Most of the energy supply and use for cooking and heating in the country is derived from fuel wood. This paper highlights the need to reduce burning wood substances as fuel since they generate carbon dioxide which is the main culprit behind the current climate change by exploiting other renewable energy sources like biofuels, solar and wind technologies. This minimizes overreliance on forests for fuel wood while still slowing down deforestation and climate change. In attaining this, the renewable energy contribution to the country’s energy generation and consumption must be improved. This paper suggests that the government in partnership with other stakeholders should design and implement effective renewable energy policies and programmes by strengthening its relevant institutions. It needs to also come up with more simpler and affordable renewable energy technologies as a pre-requisite to achieving environmental protection and sustainability. Keywords: renewable energy, fuel wood, carbon dioxide, greenhouse gas, climate change, deforestatio

Effect of addition of Nano hydroxyapatite particles on wear of resin modified glass ionomer by tooth brushing simulation

Recently, incorporation of nanohydroxyapatite (NHA) has been suggested to improve the mechanical properties of glass ionomers (GIs). This study aimed to assess the effect of addition of NHA on wear of resin modified glass ionomer (RMGI) by tooth brushing simulation. In this in vitro, experimental study, NHA in 1, 2, 5, 7 and 10wt% concentrations was added to Fuji II LC RMGI powder, and 48 samples (5×5mm) in five experimental and one control group (n=8) were fabricated. After polishing, cleaning and incubation at 37°C for three weeks, the samples were weighed and subjected to tooth brushing simulation in a toothpaste slurry according to ISO14569-1. Then, they were weighed again and the weight loss was calculated. The data were analyzed using one-way ANOVA and Tukey?s test. The highest and the lowest weight loss was found in the 0% NHA (-1.052±0.176) and 5% NHA (-0.370±0.143) groups, respectively. Wear was significantly higher in 0% NHA group (P<0.05). No difference was detected in wear between 2 and 5wt% NHA or among 1, 7 and 10wt% NHA groups. Significant differences were noted in wear between 2 and 5wt% NHA and 1, 7 and 10wt% NHA groups (P<0.001). Incorporation of up to 10wt% of NHA increases the wear resistance of Fuji II LC RMGI. This increase was the highest when 2 and 5wt% NHA were added

Effect of shark cartilage derived protein on the NK cells activity

Context: Shark cartilage has been used for its beneficial effects on various diseases. There are evidences, that shark cartilage stimulates cellular and humoral immune responses, which makes it an anti-tumor and immunomodulator candidate. Objective: The immunostimulatory effect of shark cartilage derived proteins on the cytotoxic activity of natural killer (NK) cells from healthy human peripheral blood mononuclear cells was studied. Material and methods: The shark cartilage was extracted and its bioactive proteins were purified using ion-exchange chromatography (DE-52) and sequential fractionation on Amicon ultrafiltration membranes. The effect of each protein fraction on the modulation of cytotoxic activity of NK cells, as effectors, against K562, as target cells, was assayed by enzymatic lactate dehydrogenase test. Results: The most immunostimulatory effect on the cytotoxic activity of NK cells was observed for AR10 fraction, containing proteins with molecular weight of about 14.5kDa on the reducible discontinuous sodium dodecyl sulfate polyacrylamide gel electrophoresis. Discussion: Among the examined shark cartilage derived proteins, the most immunostimulatory effects on the NK cells cytotoxicity was found for AR10 fraction with molecular weight of about 14kDa. We propose—the direct interactions of shark cartilage derived proteins with NK cells surface receptors may lead to the enhancing in the cytotoxic activity of NK cells. Conclusion: Thus AR10 fraction, proteins of about 14.5kDa, has a novel immunostimulatory effect on the NK cells activity in vitro and if confirmed by in vivo trials, it may lead to its future clinical applications as, immunotherapy of cancer, HIV, and augmentation of host immune system related immunodeficiency disorders. Keywords: Immunostimulation, purification, shark cartilage, K562, NK cel

Influence of Transformational Leadership on Knowledge Hiding: Mediating role of Organizational Psychological Ownership: A Propose Framework

Purpose: Research on knowledge hiding behaviour among employees in organizational is still in its infancy and remain scarce despite its prevalence and negative impact as reported by previous studies.Some of the possible suggested antecedents of knowledge hiding studied include trust, job characteristic, organizational culture, and work environment.Despite these studies, few have attempted to investigate the influence of transformational leadership on knowledge hiding behaviour.Furthermore, the mechanisms through which transformational leadership influences on knowledge hiding behaviour in organizations are not adequately investigated. Therefore, mediator variable is proposed.Findings: Based on transformational leadership theory and psychological ownership theory, this paper proposes that the relationship between transformational leadership and knowledge hiding behaviour of employees in the organization could be mediated by organizational psychological ownership.Implications: Thereby the organization would contain employees that are motivated and behave positively towards organizational performance

Intensity modulated radiotherapy (IMRT) with concurrent chemotherapy as definitive treatment of locally advanced esophageal cancer

Background: To report our experience with increased dose intensity-modulated radiation and concurrent systemic chemotherapy as definitive treatment of locally advanced esophageal cancer. Patients and methods: We analyzed 27 consecutive patients with histologically proven esophageal cancer, who were treated with increased-dose IMRT as part of their definitive therapy. The majority of patients had T3/4 and/or N1 disease (93%). Squamous cell carcinoma was the dominating histology (81%). IMRT was delivered in step-and-shoot technique in all patients using an integrated boost concept. The boost volume was covered with total doses of 56-60 Gy (single dose 2-2.14 Gy), while regional nodal regions received 50.4 Gy (single dose 1.8 Gy) in 28 fractions. Concurrent systemic therapy was scheduled in all patients and administered in 26 (96%). 17 patients received additional adjuvant systemic therapy. Loco-regional control, progression-free and overall survival as well as acute and late toxicities were retrospectively analyzed. In addition, quality of life was prospectively assessed according to the EORTC QLQs (QLQ-OG25, QLQ-H&N35 and QLQ-C30). Results: Radiotherapy was completed as planned in all but one patient (96%), and 21 patients received more than 80% of the planned concurrent systemic therapy. We observed ten locoregional failures, transferring into actuarial 1-, 2- and 3-year-locoregional control rates of 77%, 65% and 48%. Seven patients developed distant metastases, mainly to the lung (71%). The actuarial 1-, 2- and 3-year-disease free survival rates were 58%, 48% and 36%, and overall survival rates were 82%, 61% and 56%. The concept was well tolerated, both in the clinical objective examination and also according to the subjective answers to the QLQ questionnaire. 14 patients (52%) suffered from at least one acute CTC grade 3/4 toxicity, mostly hematological side effects or dysphagia. Severe late toxicities were reported in 6 patients (22%), mostly esophageal strictures and ulcerations. Severe side effects to skin, lung and heart were rare. Conclusion: IMRT with concurrent systemic therapy in the definitive treatment of esophageal cancer using an integrated boost concept with doses up to 60 Gy is feasible and yields good results with acceptable acute and late overall toxicity and low side effects to skin, lung and heart

The effects of delayed auditory and visual feedback on speech production

Monitoring the sensory consequences of articulatory movements supports speaking. For example, delaying auditory feedback of a speaker's voice disrupts speech production. Also, there is evidence that this disruption may be decreased by immediate visual feedback, i.e., seeing one's own articulatory movements. It is, however, unknown whether delayed visual feedback affects speech production in fluent speakers. Here, the effects of delayed auditory and visual feedback on speech fluency (i.e., speech rate and errors), vocal control (i.e., intensity and pitch), and speech rhythm were investigated. Participants received delayed (by 200 ms) or immediate auditory feedback, while repeating sentences. Moreover, they received either no visual feedback, immediate visual feedback, or delayed visual feedback (by 200, 400, and 600 ms). Delayed auditory feedback affected fluency, vocal control, and rhythm. Immediate visual feedback had no effect on any of the speech measures when it was combined with delayed auditory feedback. Delayed visual feedback did, however, affect speech fluency when it was combined with delayed auditory feedback. In sum, the findings show that delayed auditory feedback disrupts fluency, vocal control, and rhythm and that delayed visual feedback can strengthen the disruptive effect of delayed auditory feedback on fluency

Enhancing Training of Staff of the Agricultural Development Programme for Effective Agricultural Extension Service Delivery in Nigeria

This paper, identified the areas where staff of the Agricultural Development Programme (ADP) that carry out grassroots extension service delivery need to be trained and the field problems requiring research intervention. Secondary data from Annual Performance Survey (APS) report of NAERLS and NPAFS between 2010 and 2012 were used. The data were analyzed using descriptive statistics. Results showed that the most frequent key areas where trainings needed were long term course, short term refresher trainings, pre-season training, management training for administrative staff, specialized for subject matter specialist and data collection processing/analysis. The study further revealed that the development of livestock feed formula from local materials, heat-tolerant and disease resistant varieties of tomato and wheat were the most pressing problems requiring the researchers’ attention. It is recommended that ADPs should be given periodic training by relevant organizations on the identified areas and researchers should focus their researches more on the identified problems requiring research intervention. Moreover, state governments should adequately sponsor trainings of ADPs in their various states. This will strengthen the ADPs skills and increase their productivities for an effective extension service delivery in Nigeria

Mosaic Amplification of Multiple Receptor Tyrosine Kinase Genes in Glioblastoma

SummaryTumor heterogeneity has been implicated in tumor growth and progression as well as resistance to therapy. We present an example of genetic heterogeneity in human malignant brain tumors in which multiple closely related driver genes are amplified and activated simultaneously in adjacent intermingled cells. We have observed up to three different receptor tyrosine kinases (EGFR, MET, PDGFRA) amplified in single tumors in different cells in a mutually exclusive fashion. Each subpopulation was actively dividing, and the genetic changes resulted in protein production, and coexisting subpopulations shared common early genetic mutations indicating their derivation from a single precursor cell. The stable coexistence of different clones within the same tumor will have important clinical implications for tumor resistance to targeted therapies