Essential Functions of the Histone Demethylase Lid

Drosophila Little imaginal discs (Lid) is a recently described member of the JmjC domain class of histone demethylases that specifically targets trimethylated histone H3 lysine 4 (H3K4me3). To understand its biological function, we have utilized a series of Lid deletions and point mutations to assess the role that each domain plays in histone demethylation, in animal viability, and in cell growth mediated by the transcription factor dMyc. Strikingly, we find that lid mutants are rescued to adulthood by either wildtype or enzymatically inactive Lid expressed under the control of its endogenous promoter, demonstrating that Lid's demethylase activity is not essential for development. In contrast, ubiquitous expression of UAS-Lid transgenes lacking its JmjN, C-terminal PHD domain, and C5HC2 zinc finger were unable to rescue lid homozygous mutants, indicating that these domains carry out Lid's essential developmental functions. Although Lid-dependent demethylase activity is not essential, dynamic removal of H3K4me3 may still be an important component of development, as we have observed a genetic interaction between lid and another H3K4me3 demethylase, dKDM2. We also show that Lid's essential C-terminal PHD finger binds specifically to di- and trimethylated H3K4 and that this activity is required for Lid to function in dMyc-induced cell growth. Taken together, our findings highlight the importance of Lid function in the regulated removal and recognition of H3K4me3 during development

Annual (2023) taxonomic update of RNA-directed RNA polymerase-encoding negative-sense RNA viruses (realm Riboviria: kingdom Orthornavirae: phylum Negarnaviricota)

55 Pág.In April 2023, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by one new family, 14 new genera, and 140 new species. Two genera and 538 species were renamed. One species was moved, and four were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.This work was supported in part through the Laulima Government Solutions, LLC, prime contract with the U.S. National Institute of Allergy and Infec tious Diseases (NIAID) under Contract No. HHSN272201800013C. J.H.K. performed this work as an employee of Tunnell Government Services (TGS), a subcontractor of Laulima Government Solutions, LLC, under Contract No. HHSN272201800013C. U.J.B. was supported by the Division of Intramural Resarch, NIAID. This work was also funded in part by Contract No. HSHQDC15-C-00064 awarded by DHS S and T for the management and operation of The National Biodefense Analysis and Countermeasures Centre, a federally funded research and development centre operated by the Battelle National Biodefense Institute (V.W.); and NIH contract HHSN272201000040I/HHSN27200004/D04 and grant R24AI120942 (N.V., R.B.T.). S.S. acknowl edges support from the Mississippi Agricultural and Forestry Experiment Station (MAFES), USDA-ARS project 58-6066-9-033 and the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch Project, under Accession Number 1021494. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of the Army, the U.S. Department of Defence, the U.S. Department of Health and Human Services, including the Centres for Disease Control and Prevention, the U.S. Department of Homeland Security (DHS) Science and Technology Directorate (S and T), or of the institutions and companies affiliated with the authors. In no event shall any of these entities have any responsibility or liability for any use, misuse, inability to use, or reliance upon the information contained herein. The U.S. departments do not endorse any products or commercial services mentioned in this publication. The U.S. Government retains and the publisher, by accepting the article for publication, acknowledges that the U.S.Government retains a non-exclusive, paid up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for U.S. Government purposes.Peer reviewe

Influence of air flow and dehydration technique on respiration and VOCs of Pecorino grapes

The hypothesis that a controlled environmental condition of postharvest partial dehydration of wine grapes may reduce berry water stress keeping a better grape quality, was verified on white wine grapes, var. ‘Pecorino’. Grapes were harvested at 21.5% solid soluble content (SSC) and kept at 15 °C, 60% relative humidity (RH), and 2.5 m s−1 or 1.2 m s−1 air speed (tunnel), with a vapor pressure deficit (VPD) of 7.5–8 mbar; control bunches were kept in an uncontrolled condition (VPD = 0.7–6.4 mbar). Samplings for chemical and biochemical analyses were performed on grapes with 10, 25, 35, and 45% mass loss (m.l.). Tunnel and control samples reached 31.5% (in 24-26 days) and 28.5% (in 28 days) SSC, respectively. Control grapes had higher CO2 production, alcohol dehydrogenase (ADH) and lipooxygenase (LOX) activities and proline content. Ethanol and acetaldehyde contents reflected ADH activity patterns. Control grape had higher percentage of branched alcohols than control ones. An activation of aerobic fermentation in control grapes is discussed with comment on related generation of specific volatile organic compounds (VOCs)

How to do multilingual literary history? Lessons from fifteenth- and sixteenth-century north India

How can we conceptualise multingual literary culture and how can we research it? Drawing upon a 3-year AHRC funded project, this essay focuses on the materiality of the marchive, on the spaces and locations in which literature was produced and performed, and on the oral-performative practices and agents that made texts circulate to audiences in ways not bound by the script in which the texts appear to us.Not only are the models of composite culture and language specificity questioned as aresult, but the sites of literary production move from the court to a series of intersections, and areas that were peripheral move into view and connect with others