Energetic cooperation via ion-permeable junctions in mixed animal cell cultures

AbstractLow ouabain concentration (1 × 10−4 M) is shown to decrease intracellular K+ (K+in) and to increase intracellular Na+ (Na+in) in human fibroblast cell cultures. The same ouabain concentration was without effect upon K+in ad Na+in in rodent cultures such as BHK-21, mouse fibroblasts and rat glyoma C6 cells. K+in and Na+in in the mixed cultures of human and BHK-21 fibroblasts or human and mouse fibroblasts were found to be resistant to 1 × 10−4 M ouabain whereas that of the mixtures of human and rat glyoma C6 cells proved to be ouabain-sensitive. The gap-junction-mediated dye transfer was revealed between human and BHK-21 cells. Such an effect was very small in the human-C6 cell mixed culture. It is concluded that cells with active ion pumps can support the maintenance of K+ and Na+ gradients in cells with inactive pumps, provided that effective ion transport via gap junctions takes place

The comparative responsiveness of Hospital Universitario Princesa Index and other composite indices for assessing rheumatoid arthritis activity

Objective To evaluate the responsiveness in terms of correlation of the Hospital Universitario La Princesa Index (HUPI) comparatively to the traditional composite indices used to assess disease activity in rheumatoid arthritis (RA), and to compare the performance of HUPI-based response criteria with that of the EULAR response criteria. Methods Secondary data analysis from the following studies: ACT-RAY (clinical trial), PROAR (early RA cohort) and EMECAR (pre-biologic era long term RA cohort). Responsiveness was evaluated by: 1) comparing change from baseline (Delta) of HUPI with Delta in other scores by calculating correlation coefficients; 2) calculating standardised effect sizes. The accuracy of response by HUPI and by EULAR criteria was analyzed using linear regressions in which the dependent variable was change in global assessment by physician (Delta GDA-Phy). Results Delta HUPI correlation with change in all other indices ranged from 0.387 to 0.791); HUPI's standardized effect size was larger than those from the other indices in each database used. In ACT-RAY, depending on visit, between 65 and 80% of patients were equally classified by HUPI and EULAR response criteria. However, HUPI criteria were slightly more stringent, with higher percentage of patients classified as non-responder, especially at early visits. HUPI response criteria showed a slightly higher accuracy than EULAR response criteria when using Delta GDA-Phy as gold standard. Conclusion HUPI shows good responsiveness in terms of correlation in each studied scenario (clinical trial, early RA cohort, and established RA cohort). Response criteria by HUPI seem more stringent than EULAR''s

Pharmacology of a new medicinal form, phenasal granules

Experiments on mice and rats were made to study a new medicinal form, phenasal granules given per os and to compare it with ground tablets and powder of phenasal as regards the intestinal content, absorption capacity, anthelminthic activity and toxicity. The intestinal content and absorption capacity of phenasal were medicinal form-dependent. The highest anthelminthic activity was exhibited by phenasal granules. Acute toxicity of powder, ground tablets and granules of phenasal administered to mice and rats per os remained the same

Pharmacology of a new medicinal form, phenasal granules

Experiments on mice and rats were made to study a new medicinal form, phenasal granules given per os and to compare it with ground tablets and powder of phenasal as regards the intestinal content, absorption capacity, anthelminthic activity and toxicity. The intestinal content and absorption capacity of phenasal were medicinal form-dependent. The highest anthelminthic activity was exhibited by phenasal granules. Acute toxicity of powder, ground tablets and granules of phenasal administered to mice and rats per os remained the same

Морфологічні особливості Bidens сonnata Muehl. ex Willd. у різних частинах вторинного ареалу (європейська частина Росії, Україна)

A comparative analysis of morphological characte ristics of Bidens connata Muehl. ex Willd. in Russia (Kaluga and Kaliningrad regions) and in Ukraine (Kyiv and Poltava regions) are made. B. connata grows in ruderal habitats. This species settled in natural plant communities (meadows in alluvial lands). We found wide variability in morphological parameters of B. connata growing in different localities of the area.Проведено порівняльний аналіз морфологічних ознак Bidens connata Muehl. ex Willd. на території Росії (Калузька і Калінінградська обл. ) та України (Київська і Полтавська обл.). Вид зростає у рудеральних місцях, а також у малопорушених фітоценозах лучної і заплавної рослинності. Виявлено широку варіабельність морфологічних показників B. connata в різних частинах ареалу

Докозагексаеновая кислота в лечении мужского бесплодия

Literature review is devoted to the analysis of modern data on the use of docosahexaenoic acid in the treatment of male infertility. A brief description of modern ideas about the possible causes of male infertility (a disturbance of function of the cell membrane and genetic damage of sperm) was conducted. The data on the anti-inflammatory properties of omega-3 polyunsaturated fatty acids are described in detail. The bioavailability of docosahexaenoic acid and the choice of drugs containing it for the treatment of male infertility are discussed. Increasing the content of polyunsaturated fatty acids, in particular docosahexaenoic acid, in the sperm membrane was associated with higher ejaculate quality. Therapy with the use of these substances led to the improvement of standard indicators of semen and a decrease in the proportion of sperm with fragmented DNA.Обзор литературы посвящен анализу современных сведений о применении докозагексаеновой кислоты при лечении мужского бесплодия. Дано краткое описание современных представлений о возможных причинах мужского бесплодия — нарушении функции клеточной мембраны и повреждении генетического материала сперматозоидов. Подробно изложены данные о противовоспалительных свойствах омега-3-полиненасыщенных жирных кислот. Обсуждаются вопросы биодоступности докозагексаеновой кислоты и выбора содержащих ее препаратов для терапии мужского бесплодия. Повышение содержания полиненасыщенных жирных кислот, в частности докозагексаеновой кислоты, в мембране сперматозоидов было ассоциировано с более высокими показателями качества эякулята. Терапия с применением этих веществ приводила к улучшению стандартных показателей спермограммы и снижению доли сперматозоидов с фрагментированной ДНК

Azafluorenols and their antihistamine activity

[No abstract available