Mapping Of A Novel Viviparous Unstable Mutant Of Maize (up12)

A new viviparous mutant of maize (Zea mays L.), associated with genetic instability and designated viviparous-12 (vp12), was identified in a synthetic Tuxpeno adapted to tropical regions. In the present work, the linkage group of this new locus was determined. Progenies of inbred line L477 segregating for the vp12 mutant were crossed with waxy-marked reciprocal translocation stocks. The phenotypic frequencies of the wx and vp12 mutants were analyzed in F2 progenies. The results demonstrated that the Viviparous-12 locus of maize is located on the long arm of chromosome 6.201717

Non-destructive Assessment Of Chlorophyll-deficient Mutants Of Maize (zea Mays L.) By Photoacoustic Spectroscopy

Photoacoustic spectroscopy is an efficient tool for the non-destructive assessment of chlorophyll and carotenoid deficient mutants of Zea mays L. Intact leaf samples taken from the mutants Albina, Luteus I and Luteus II were compared with those from the corresponding isogenic normals. The results are compared with those obtained applying some commonly used destructive techniques. © 1985.3814751Prioli, (1981) M.Sc. Thesis, , 2nd edn., State University of Campinas, San Paulo, Brazil, (in Portuguese)Holden, Chlorophylls (1976) Chemistry and Biochemistry of Plant Pigments, 2. , 2nd edn., T.W. Goodwin, Academic Press, New YorkDavies, Carotenoids (1976) Chemistry and Biochemistry of Plant Pigments, 2. , 2nd edn., T.W. Goodwin, Academic Press, New YorkBell, (1980) Am. J. Sci., 20, p. 305Viengerov, (1938) Dokl. Akad. Nauk SSSR, 19, p. 687Rosencwaig, (1973) Science, 181, p. 657Pao, (1980) Optoacoustic spectroscopy and detection, , Academic Press, New YorkRosencwaig, (1980) Photoacoustics and Photoacoustic Spectroscopy, , J. Wiley and Sons, New YorkKinney, Staley, (1982) Annu. Rev. Mater. Sci., 12, p. 295McClelland, Photoacoustic spectroscopy (1983) Analytical Chemistry, 55, p. 89Cahen, Bults, Garty, Malkin, (1980) J. Biochem. Biophys. Methods, 3, p. 293Lima, Mattos, Miranda, Penna, von Bulow, Ghizoni, (1982) J. Photoacoustics, 1, p. 61Lasser-Ross, Malkin, Cahen, (1980) Biochim. Biophys. Acta, 593, p. 330Bults, Horwitz, Malkin, Cahen, (1982) Biochim. Biophys. Acta, 679, p. 452Poulet, Cahen, Malkin, (1983) Biochim. Biophys. Acta, 724, p. 433Kanstad, Cahen, Malkin, (1983) Biochim. Biophys. Acta, 722, p. 18

In Vivo And In Situ Measurements Of Spectroscopic And Photosynthesis Properties Of Undetached Maize Leaves Using The Open Photoacoustic Cell Technique

A new and highly sensitive method, based on the photoacoustic effect, is described for in vivo and in situ studies of photosynthetic activity of undetached leaves. The general utility of this simple photothermal method is demonstrated by examining the spectroscopy and photosynthetic activity of green, striped, and albino plants of maize (Zea mays L.). © 1994.961-2203209Rosencwaig, (1980) Photoacoustics and photoacoustic spectroscopy, , Wiley, New YorkVargas, Miranda, Photoacoustic and related photothermal techniques (1988) Phys. Rep., 161, pp. 45-101Anjo, Moore, A photoacoustic depth profile of β-carotene in skin (1984) Photochem. Photobiol., 39, p. 635Cesar, Vargas, Miranda, Photoacoustic microscopy of layered samples: phase detection technique (1985) J. Phys. D: Appl. Phys., 18, pp. 599-608Adams, Kirkbright, Analytical optoacoustic spectrometryPart III: The optoacoustic effect and thermal diffusivity (1977) Analyst, 102, pp. 281-292Adams, Kirkbright, Thermal diffusivity and thickness measurements for solid samples utilizing the optoacoustic effect (1977) Analyst, 102, pp. 678-682Pessoa, Jr., Cesar, Patel, Vargas, Ghizoni, Miranda, Two-beam photoacoustic phase measurement of the thermal diffusivity of solids (1986) J. Appl. Physiol., 59, pp. 1316-1318Leite, Cella, Vargas, Miranda, Photoacoustic measurement of thermal diffusivity of polymer foils (1987) J. Appl. Phys., 61, pp. 3025-3027Merkle, Powell, Photoacoustic spectroscopy investigation of radiationless transitions in Eu2+ ions in KCl crystals (1977) Chem. Phys. Lett., 46, pp. 303-306Quimby, Yen, Photoacoustic measurement of the ruby quantum efficiency (1980) J. Appl. Physiol., 51, pp. 1780-1782Lima, Baesso, Arguello, Silva, Vargas, Miranda, Phase-resolved photoacoustic spectroscopy: application to metallic-ion-doped glasses (1987) Physiol. Rev., B, 36, pp. 9812-9815Marquezini, Cella, Mansanares, Vargas, Miranda, Open photoacoustic cell spectroscopy (1991) Meas. Sci. Technol., 2, pp. 396-401Pereira, Zerbetto, Silva, Vargas, da Silva, de O. Neto, Cella, Miranda, OPC technique for in vivo studies in plant photosynthesis research (1992) Meas. Sci. Technol., 3, pp. 931-934Lima, Vargas, Cesar, Lima, Prioli, da Silva, Non-destructive assessment of chlorophyll-deficient mutants of maize (Zea mays L.) by photoacoustic spectroscopy (1985) Plant Sci., 38, pp. 47-51Poulet, Cahen, Malkin, Photoacoustic detection of photosynthetic oxygen evolution from leaves. Quantitative analysis by phase and amplitude measurements (1983) Biochim. Biophys. Acta, 724, pp. 433-446Bults, Horwitz, Malkin, Cahen, Photoacoustic measurements of photosynthetic activities in whole leaves: Photochemistry and gas exchange (1982) Biochem. Biophys. Acta, 679, pp. 452-465Canaani, Cahen, Malkin, Photoacoustic as a probe for photosynthetic O2 evolution and energy storage in an intact leaf-distribution of excitation energy between PSII and PSI (1984) Advances in Photosynthesis Research, 3, pp. 331-334. , C. Sybesma, Martinus Nijhoff/Dr. W. Junk Publishers, The HagueSnel, Kooijman, Vredenberg, Correlation between chlorophyll fluorescence and photoacoustic signal transients in spinach leaves (1990) Photosynth. Res., 25, pp. 259-268Fork, Herbert, The application of photoacoustic techniques to studies of photosynthesis (1993) Photochemistry and Photobiology, 57, pp. 207-220Maluf, Study of a carotenoid-deficient and vivaparous mutant caused by transposon in Zea mays L. (1991) M.Sc. Thesis, , State University of Campinas, Campinas, SP, Brazil, (in Portuguese)Pereira, de Oliveira Neto, Vargas, Cella, Miranda, On the use of OPC technique for studying photosynthetic O2 evolution of undetached leaves: comparison with Clark-type O2 electrode (1993) Rev. Sci. Instr., , in pres

Photosynthetic O2 Evolution In Maize Inbreds And Their Hybrids Can Be Differentiated By Open Photoacoustic Cell Technique

Photosynthetic efficiency is considered one of the traits potentially suitable to differentiate hybrids from their inbred lines. Previous evaluations concerning photosynthetic efficiencies of maize (Zea mays L.) plants in the field have shown inconsistent and contradictory data. In this work, we attempted to study photosynthetic O2 evolution through photoacoustic spectroscopy in intact undetached leaves of dark-adapted seedlings of inbreds and their hybrids. The results indicate that photosynthetic efficiencies of inbreds and hybrids can be differentiated by photoacoustic measurements of oxygen evolution, providing a parameter that might prove useful in evaluating plant genotypes. © 1995.1042177181Shull, A pure-line method in corn breeding (1909) Am. Breeders Assoc. Rep., 5, pp. 51-59Duvick, Genetic contribution to the yield gains of U.S. hybrid maize, 1930 to 1980 (1984) Genetic Contributions to Yield gains of Five Major Crop Plants, 7, pp. 15-47. , 2nd Edn., W.R. Fehr, Crop Science Society of America Special PublicationRussell, Contribution of breeding to maize improvement in the United States (1986) Iowa State J. Res., 61, pp. 5-34Smith, Smith, Measurement of genetic diversity among maize hybrids: a comparison of isozymic, RFLP, pedigree, and heterosis data (1992) Maydica, 37, pp. 53-60Stuber, Lincoln, Wolff, Helentjaris, Lander, Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers (1992) Genetics, 132, pp. 823-839Moss, Photosynthesis the corn plant a converter (1960) Proceedings of the 15th Annual Hybrid Corn Industry — Research Conference Publication, 15, pp. 54-60. , 2nd EdnElmore, Hesketh, Muramoto, A survey of rates of leaf growth leaf aging and photosynthetic rates among and within species (1967) Journal of the Arizona Academy of Science, 4, pp. 215-219Heichel, Musgrave, Varietal differences in net photosynthesis of Zea mays L (1969) Crop Science, 9, pp. 483-496Marquezini, Cella, Mansanares, Vargas, Miranda, Open photoacoustic cell spectroscopy (1991) Meas. Sci. Technol., 2, pp. 396-401Pereira, Zerbetto, Silva, Vargas, da Silva, Neto, Cella, Miranda, OPC technique for in vivo studies in plant photosynthesis research (1992) Meas. Sci. Technol., 3, pp. 931-934Pereira, Prioli, da Silva, Neto, Vargas, Cella, Alvarado-Gil, In vivo and in situ measurements of spectroscopic and photosynthetic properties of undetached maize leaves using the open photoacoustic cell technique (1994) Plant Sci., 96, pp. 203-209Poulet, Cahen, Malkin, Photoacoustic detection of photosynthetic oxygen evolution from leaves. Quantitative analysis by phase and amplitude measurements (1983) Biochim. Biophys. Acta, 724, pp. 433-446Walker, Photosynthetic induction phenomena and the light activation of ribulose diphosphate carboxylase (1973) New Phytologist, 72, pp. 209-23

Determination of aspartate kinase in maize tissues.

Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity