211 research outputs found
ÍNDICES PROLIFERATIVOS DO TUMOR VENÉREO CANINO TRANSMISSÍVEL PELAS TÉCNICAS DO CEC E KI-67 NA CITOLOGIA ASPIRATIVA COM AGULHA FINA
A citologia aspirativa com agulha fina (CAAF) foi usada como método de colheita de
quinze amostras de tumor venéreo transmissível (TVT)em cães, oriundos do atendimento do Hospital
Veterinário-FMVZ-UNESP de Botucatu, antes e após o início da quimioterapia. As amostras
citológicas foram submetidas ao cálculo do índice mitótico e à avaliação de índices de proliferação
por métodos citoquímico (concentração eletrolítica crítica CEC) e imunocitoquímico (Ki-67, clone
MIB-1). Todas as técnicas mostraram-se adequadas para avaliar a taxa proliferativa em amostras
citológicas. O CEC permitiu observar que a aplicação do quimioterápico levou a uma redução
significativa da proliferação celular. Em vista disso, conclui-se que essa metodologia pode ser aplicada
para diagnosticar e monitorar o TVT.
Proliferation indexes determination by CEC and Ki-67 in fine needle aspiration
cytology of transmissible venereal tumor
Abstract
Fine needle aspiration biopsy (FNAB) was used to collect fifteen transmissible venereal
tumor (TVT) samples from dogs at the Veterinary Hospital - FMVZ - UNESP Botucatu, São Paulo,
Brazil, before and after beginning of chemotherapy. The cytological samples were submitted to
determination of mitotic and proliferation indexes by means of cytochemical (critical electrolyte
concentration CEC) and immunocytochemical (Ki-67, MIB-1 clone) methods. All the techniques
were adequate for the evaluation of proliferation rates displayed by the cytological samples. The
CEC allowed the observation that chemotherapy induces to significant reduction in cellular proliferation.
Thus, was concluded that this methodology is suitable to perform TVT diagnosis and monitoration
Testes para avaliação do vigor de sementes de sorgo com ênfase à condutividade elétrica
Burden and risk factors for Pseudomonas aeruginosa community-acquired pneumonia:a Multinational Point Prevalence Study of Hospitalised Patients
Pseudornonas aeruginosa is a challenging bacterium to treat due to its intrinsic resistance to the antibiotics used most frequently in patients with community-acquired pneumonia (CAP). Data about the global burden and risk factors associated with P. aeruginosa-CAP are limited. We assessed the multinational burden and specific risk factors associated with P. aeruginosa-CAP.
We enrolled 3193 patients in 54 countries with confirmed diagnosis of CAP who underwent microbiological testing at admission. Prevalence was calculated according to the identification of P. aeruginosa. Logistic regression analysis was used to identify risk factors for antibiotic-susceptible and antibiotic-resistant P. aeruginosa-CAP.
The prevalence of P. aeruginosa and antibiotic-resistant P. aeruginosa-CAP was 4.2% and 2.0%, respectively. The rate of P. aeruginosa CAP in patients with prior infection/colonisation due to P. aeruginosa and at least one of the three independently associated chronic lung diseases (i.e. tracheostomy, bronchiectasis and/or very severe chronic obstructive pulmonary disease) was 67%. In contrast, the rate of P. aeruginosa-CAP was 2% in patients without prior P. aeruginosa infection/colonisation and none of the selected chronic lung diseases. The multinational prevalence of P. aeruginosa-CAP is low.
The risk factors identified in this study may guide healthcare professionals in deciding empirical antibiotic coverage for CAP patients
Adaptação do teste de tetrazólio para avaliação da viabilidade e do vigor de sementes de girassol
Estrutura fitossociológica, estoques de volume, biomassa, carbono e dióxido de carbono em Floresta Estacional Semidecidual
Comportamento biológico de matriz scaffold acrescida de células progenitoras na reparação óssea
The LHCb upgrade I
The LHCb upgrade represents a major change of the experiment. The detectors have been almost completely renewed to allow running at an instantaneous luminosity five times larger than that of the previous running periods. Readout of all detectors into an all-software trigger is central to the new design, facilitating the reconstruction of events at the maximum LHC interaction rate, and their selection in real time. The experiment's tracking system has been completely upgraded with a new pixel vertex detector, a silicon tracker upstream of the dipole magnet and three scintillating fibre tracking stations downstream of the magnet. The whole photon detection system of the RICH detectors has been renewed and the readout electronics of the calorimeter and muon systems have been fully overhauled. The first stage of the all-software trigger is implemented on a GPU farm. The output of the trigger provides a combination of totally reconstructed physics objects, such as tracks and vertices, ready for final analysis, and of entire events which need further offline reprocessing. This scheme required a complete revision of the computing model and rewriting of the experiment's software
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