481 research outputs found
ΠΠΎΠ½ΡΡΠΎΠ»Ρ ΠΊΠ°ΡΠ΅ΡΡΠ²Π° ΠΌΠ΅ΡΠ΅Π½ΡΡ FITC Π±Π΅Π»ΠΊΠΎΠ² Π΄Π»Ρ ΠΈΠ½ΡΠ΅ΡΠ°ΠΊΡΠΎΠΌΠ½ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ SDS Π³Π΅Π»Ρ-ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·Π° ΠΈ SPR Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠΎΠΌ
The technology of dye-labeled proteins has many fields of application, especially in interactomics. The aim of this work was to adapt protocol of conjugation of low molecular weight (12 β 15 kDΠ°) heme-containing proteins with fluorescein isothiocyanate, isomer I, (FITC) for subsequent protein-protein interaction studies. We have monitored the quality of FITC-labeling of the target protein and comparative assessment of its binding capacity. Using the cytochrome C (Mw 12 kDΠ°) as an example, it has been shown that using the three step method approach including conventional spectrophotometry, capillary gel electrophoresis and SPR analysis it is possible to assess: (i) the capability of the FITC-labeled target protein to interact with its protein partner and protein material from tissue lysates, (ii) the fact of dye conjugation with the protein, and (iii) the quality of purification for final protein preparation from unreacted free dye moleculesΠ’Π΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡ ΠΌΠ΅ΡΠ΅Π½ΡΡ
ΠΊΡΠ°ΡΠΈΡΠ΅Π»Π΅ΠΌ Π±Π΅Π»ΠΊΠΎΠ² ΠΈΠΌΠ΅Π΅Ρ ΠΎΡΠ΅Π½Ρ ΠΌΠ½ΠΎΠ³ΠΎ ΠΎΠ±Π»Π°ΡΡΠ΅ΠΉ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΠΈ Π΄Π»Ρ ΠΈΠ½ΡΠ΅ΡΠ°ΠΊΡΠΎΠΌΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ. Π¦Π΅Π»ΡΡ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»Π° ΠΎΡΠ΅Π½ΠΊΠ° ΠΏΡΠΈΠΌΠ΅Π½ΠΈΠΌΠΎΡΡΠΈ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Π° ΠΌΠ΅ΡΠ΅Π½ΠΈΡ Π±Π΅Π»ΠΊΠΎΠ² ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅ΠΈΠ½ ΠΈΠ·ΠΎΡΠΈΠΎΡΠΈΠΎΠ½Π°ΡΠΎΠΌ (ΠΈΠ·ΠΎΠΌΠ΅Ρ I, (FITC)) Π΄Π»Ρ Π±Π΅Π»ΠΊΠΎΠ² Ρ Π½Π΅Π±ΠΎΠ»ΡΡΠΎΠΉ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠΉ ΠΌΠ°ΡΡΠΎΠΉ (12 β15 ΠΊΠΠ°) ΠΏΡΡΠ΅ΠΌ ΠΈΡ
ΠΊΠΎΠ²Π°Π»Π΅Π½ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΈΠΈ Ρ FITC, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ ΠΊΠ°ΡΠ΅ΡΡΠ²Π° Π²ΠΊΠ»ΡΡΠ΅Π½ΠΈΡ ΠΌΠ΅ΡΠΊΠΈ Π² Π±Π΅Π»ΠΎΠΊ ΠΈ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½Π°Ρ ΠΎΡΠ΅Π½ΠΊΠ° Π΅Π³ΠΎ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΊ Π±Π΅Π»ΠΎΠΊ-Π±Π΅Π»ΠΊΠΎΠ²ΡΠΌ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡΠΌ. ΠΠ° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Ρ (12 ΠΊΠΠ°) Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΊΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΠΈ, ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π»Ρ-ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·Π° ΠΈ SPR-Π°Π½Π°Π»ΠΈΠ·Π° ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΠ΄Π΅Π»Π°ΡΡ Π²ΡΠ²ΠΎΠ΄Ρ ΠΎ: Π°) ΡΠΎΡ
ΡΠ°Π½Π΅Π½ΠΈΠΈ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΌΠ΅ΡΠ΅Π½ΠΎΠ³ΠΎ ΡΠ΅Π»Π΅Π²ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΎΠ²Π°ΡΡ Ρ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ-ΠΏΠ°ΡΡΠ½ΡΡΠ°ΠΌΠΈ ΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΠΌ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠΌ ΡΠΊΠ°Π½Π΅Π²ΡΡ
Π»ΠΈΠ·Π°ΡΠΎΠ²; Π±) ΡΠ°ΠΊΡΠ΅ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΈΡ ΠΌΠ΅ΡΠΊΠΈ Π² Π±Π΅Π»ΠΎΠΊ; Π²) ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΎΡΠΈΡΡΠΊΠΈ ΡΠΈΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΠΎΡ Π½Π΅ ΠΏΡΠΎΡΠ΅Π°Π³ΠΈΡΠΎΠ²Π°Π²ΡΠΈΡ
Ρ Π½ΠΈΠΌ ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΡΡ
ΠΌΠΎΠ»Π΅ΠΊΡΠ» ΠΊΡΠ°ΡΠΈΡΠ΅Π»Ρ
ΠΡΡΠΎΠΊΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ° Π½ΠΈΠ·ΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ Π½Π° Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Ρ CYP51 Candida krusei
The opportunistic fungus Candida krusei is the causative agent of nosocomial infections characterized by high mortality and development of resistance to drugs of the azole class. Therefore, develjoment of non-azole antifungal agents against resistant fungal strains is extremly important. Lanosterol 14-alpha demethylase (CYP51) is a well-known antifungal target. The optical SPR biosensor is a universal tool for screening studies in search of new drug prototypes. This paper presents the methodological aspects of high-hroughput SPR based screening of a library of low molecular weight compounds of natural origin for their interaction with C. krusei CYP51. It has been shown that when performing high-throughput screening, a researcher should pay special attention to the degree of a sensorgram curvature in the association phase. The described approaches to the analysis of high throughput screening data can be useful for researchers working with SPR biosensors from various manufacturers.Π£ΡΠ»ΠΎΠ²Π½ΠΎ-ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΠΉ Π³ΡΠΈΠ± Candida krusei (C. krusei) ΡΠ²Π»ΡΠ΅ΡΡΡ Π²ΠΎΠ·Π±ΡΠ΄ΠΈΡΠ΅Π»Π΅ΠΌ Π½ΠΎΠ·ΠΎΠΊΠΎΠΌΠΈΠ°Π»ΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ, Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΠΈΡ
ΡΡ Π²ΡΡΠΎΠΊΠΎΠΉ Π»Π΅ΡΠ°Π»ΡΠ½ΠΎΡΡΡΡ. Π ΠΏΠΎΡΠ»Π΅Π΄Π½Π΅Π΅ Π²ΡΠ΅ΠΌΡ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΡ ΠΊ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ Π΄ΠΎΠ»ΠΈ ΡΡΠ°ΠΌΠΌΠΎΠ² Π΄ΡΠΎΠΆΠΆΠ΅Π²ΡΡ
Π³ΡΠΈΠ±ΠΎΠ², ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΡΡ
ΠΊ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°ΠΌ ΠΊΠ»Π°ΡΡΠ° Π°Π·ΠΎΠ»ΠΎΠ². ΠΠΎΡΡΠΎΠΌΡ ΠΏΠΎΠΈΡΠΊ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ
ΠΏΡΠΎΡΠΈΠ²ΠΎΠ³ΡΠΈΠ±ΠΊΠΎΠ²ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ Π½Π΅Π°Π·ΠΎΠ»ΡΠ½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΠΌ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΡΠΈ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ΅ Π½ΠΎΠ²ΡΡ
ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΡΠ΅Π΄ΡΡΠ² Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΡΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² Π³ΡΠΈΠ±ΠΎΠ². ΠΠ°Π½ΠΎΡΡΠ΅ΡΠΎΠ» 14-Π°Π»ΡΡΠ° Π΄Π΅ΠΌΠ΅ΡΠΈΠ»Π°Π·Π° (CYP51) ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΈΡΠΎΠΊΠΎ ΠΈΠ·Π²Π΅ΡΡΠ½ΠΎΠΉ ΠΌΠΈΡΠ΅Π½ΡΡ Π΄Π»Ρ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ³ΡΠΈΠ±ΠΊΠΎΠ²ΡΡ
ΡΡΠ΅Π΄ΡΡΠ². ΠΠΏΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ ΡΠΎΠ±ΠΎΠΉ ΡΠ½ΠΈΠ²Π΅ΡΡΠ°Π»ΡΠ½ΡΠΉ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½Ρ Π΄Π»Ρ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ΠΎΠ²ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ, ΡΠ΅Π»ΡΡ ΠΊΠΎΡΠΎΡΡΡ
ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠΎΠΈΡΠΊ ΠΏΡΠΎΡΠΎΡΠΈΠΏΠΎΠ² Π½ΠΎΠ²ΡΡ
Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ. Π Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π°ΡΠΏΠ΅ΠΊΡΡ Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ Π½ΠΈΠ·ΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΠΏΡΠΈΡΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ, Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ Π½Π° ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠ΅ ΠΈΡ
Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Ρ CYP51 C. krusei Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ°. ΠΠ°ΠΊ ΠΏΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ, ΠΏΡΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΈ Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΎΡΠΎΠ±ΠΎΠ΅ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΡΠ»Π΅Π΄ΡΠ΅Ρ ΠΎΠ±ΡΠ°ΡΠ°ΡΡ Π½Π° ΡΡΠ΅ΠΏΠ΅Π½Ρ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π½Π°ΠΊΠ»ΠΎΠ½Π° ΡΠ΅Π½ΡΠΎΠ³ΡΠ°ΠΌΠΌΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Π² ΡΠ°Π·Π΅ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ. ΠΠΏΠΈΡΠ°Π½Π½ΡΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ ΠΊ Π°Π½Π°Π»ΠΈΠ·Ρ Π΄Π°Π½Π½ΡΡ
Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΏΠΎΠ»Π΅Π·Π½Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠΌ, ΡΠ°Π±ΠΎΡΠ°ΡΡΠΈΠΌ Ρ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ°ΠΌΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΌΠΎΠ΄Π΅Π»Π΅ΠΉ
SolCyc: a database hub at the Sol Genomics Network (SGN) for the manual curation of metabolic networks in Solanum and Nicotiana specific databases
SolCyc is the entry portal to pathway/genome databases (PGDBs) for major species of the Solanaceae family hosted at the Sol Genomics Network. Currently, SolCyc comprises six organism-specific PGDBs for tomato, potato, pepper, petunia, tobacco and one Rubiaceae, coffee. The metabolic networks of those PGDBs have been computationally predicted by the pathologic component of the pathway tools software using the manually curated multi-domain database MetaCyc (http://www.metacyc.org/) as reference. SolCyc has been recently extended by taxon-specific databases, i.e. the family-specific SolanaCyc database, containing only curated data pertinent to species of the nightshade family, and NicotianaCyc, a genus-specific database that stores all relevant metabolic data of the Nicotiana genus. Through manual curation of the published literature, new metabolic pathways have been created in those databases, which are complemented by the continuously updated, relevant species-specific pathways from MetaCyc. At present, SolanaCyc comprises 199 pathways and 29 superpathways and NicotianaCyc accounts for 72 pathways and 13 superpathways. Curator-maintained, taxon-specific databases such as SolanaCyc and NicotianaCyc are characterized by an enrichment of data specific to these taxa and free of falsely predicted pathways. Both databases have been used to update recently created Nicotiana-specific databases for Nicotiana tabacum, Nicotiana benthamiana, Nicotiana sylvestris and Nicotiana tomentosiformis by propagating verifiable data into those PGDBs. In addition, in-depth curation of the pathways in N.tabacum has been carried out which resulted in the elimination of 156 pathways from the 569 pathways predicted by pathway tools. Together, in-depth curation of the predicted pathway network and the supplementation with curated data from taxon-specific databases has substantially improved the curation status of the species\u2013specific N.tabacum PGDB. The implementation of this strategy will significantly advance the curation status of all organism-specific databases in SolCyc resulting in the improvement on database accuracy, data analysis and visualization of biochemical networks in those species
Photoconductivity in macroporous silicon with regular structure of macropores
The effects of the increase of photoconductivity in periodic macroporous
silicon structures depending on the size and period of cylindrical macropores are
investigated. It is obtained that the ratio of macroporous silicon photoconductivity to
bulk silicon photoconductivity achieves a maximum at the distance between macropores
equal to two thicknesses of the Schottky layer, which corresponds to the experimental
data. The increase of photoconductivity is due to both the large total surface area of
macropores and the existence of Schottky layers in the near-surface region of cylindrical
macropores
Out-of-plane optical transmittance of 2D photonic macroporous silicon structures
Optical transmission spectra of 2D photonic macroporous silicon structures are
investigated. The absolute bandgap for high values of the out-of-plane component kz is
situated between the second and third photonic bands. Essential reduction in the
transmittance of electromagnetic radiation and the step formation are observed for
wavelengths less than the optical period of structures due to directed and decay optical
modes formed by macroporous silicon as a short waveguide. The absorption in the
macroporous silicon structure is determined by a maximum of the longitudinal
component of electromagnetic waves, its interaction with 2D surface oscillations, and the
appearance of polaritonic resonances
ΠΠ£ΠΠΠΠΠΠ¦ΠΠΠΠΠΠ― ΠΠΠ’ΠΠΠΠΠ‘Π’Π¬ Π ΠΠ‘Π‘ΠΠΠ‘ΠΠΠ ΠΠΠΠΠ¦ΠΠΠ‘ΠΠΠ ΠΠΠ£ΠΠ Π Π€ΠΠΠ£Π‘Π ΠΠΠ’Π£ΠΠΠ¬ΠΠΠ ΠΠΠ£Π§ΠΠΠ ΠΠΠΠΠ’ΠΠΠ: ΠΠ¦ΠΠΠΠ ΠΠΠ‘Π’ΠΠΠΠΠΠ‘Π’Π Π¦ΠΠΠΠΠ«Π₯ ΠΠΠΠΠΠΠ’ΠΠΠΠ
A comprehensive review of National research policy papers issued over the past 6 years was carried out. A set of problems concerning the quality of predicted values of some bibliometric indicators reflecting the level of research performance and publication activity that were declared in governmental documents was discussed. Basic metrics of scientific performance that should be required to achieve the goals declared in the recent governmental policy papers including Presidentβs Executive Order βΒ 599 of May 7, 2012 (increasing the share of Russian researchersβ publications in the total number of publications in international scientific journals indexed in the Web of Science up to 2,44% in 2015). Taking into account the current structure of modern global science in which papers in biomedical subjects make up for approximately one third of the total world scientific output, it becomes obvious how difficult is the governmental task set up to the researchers β to double the number of journal publications indexed in Web of Science in the short-term period of the nearest three years. The priorities and reasonable goal-oriented efforts to meet the targets are proposed in the paper.Β ΠΡΠΎΠ²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ· Π³ΠΎΡΡΠ΄Π°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ½ΡΡ
Π΄ΠΎΠΊΡΠΌΠ΅Π½ΡΠΎΠ² ΠΏΠΎ Π½Π°ΡΡΠ½ΠΎΠΉ ΠΏΠΎΠ»ΠΈΡΠΈΠΊΠ΅, Π² ΠΊΠΎΡΠΎΡΡΡ
ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈΡΡ ΠΈΠ½Π΄ΠΈΠΊΠ°ΡΠΎΡΡ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈ ΠΏΡΠΎΠ³Π½ΠΎΠ·Π½ΡΠ΅ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠΎΡΡΠ° ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
Π½Π°ΡΡΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ. Π Π°ΡΡΡΠΈΡΠ°Π½Ρ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, ΠΊΠΎΡΠΎΡΡΠ΅ Π΄ΠΎΠ»ΠΆΠ½Ρ Π±ΡΡΡ Π΄ΠΎΡΡΠΈΠ³Π½ΡΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΌ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΠΌ ΡΠΎΠΎΠ±ΡΠ΅ΡΡΠ²ΠΎΠΌ, ΡΡΠΎΠ±Ρ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΎΠ²Π°ΡΡ ΡΠ΅Π»Π΅Π²ΡΠΌ Π±ΠΈΠ±Π»ΠΈΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΈΠ½Π΄ΠΈΠΊΠ°ΡΠΎΡΠ°ΠΌ, Π·Π°ΡΠ²Π»Π΅Π½Π½ΡΠΌ Π² Π£ΠΊΠ°Π·Π΅ ΠΡΠ΅Π·ΠΈΠ΄Π΅Π½ΡΠ° βΒ 599 ΠΎΡ 7 ΠΌΠ°Ρ 2012Β Π³. (ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ Π΄ΠΎΠ»ΠΈ ΡΠΎΡΡΠΈΠΉΡΠΊΠΈΡ
ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΉ Π² Web of Science Π΄ΠΎ 2,44% ΠΊ 2015Β Π³.), ΠΈ Π² Β«Π‘ΡΡΠ°ΡΠ΅Π³ΠΈΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠΉ Π½Π°ΡΠΊΠΈ Π΄ΠΎ 2025Β Π³.Β» ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π³Π»ΠΎΠ±Π°Π»ΡΠ½Π°Ρ Π½Π°ΡΠΊΠ°, Π² ΡΠ»ΠΎΠΆΠΈΠ²ΡΠ΅ΠΉΡΡ ΡΡΡΡΠΊΡΡΡΠ΅ ΠΊΠΎΡΠΎΡΠΎΠΉ Π½Π° Π±ΠΈΠΎΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΠ΅ ΡΡΠ°ΡΡΠΈ ΠΏΡΠΈΡ
ΠΎΠ΄ΠΈΡΡΡ ΠΎΠΊΠΎΠ»ΠΎ 1/3 ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΌΠΈΡΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΠΊΠ°, ΡΡΠ°Π²ΠΈΡ ΠΏΠ΅ΡΠ΅Π΄ ΡΠΎΡΡΠΈΠΉΡΠΊΠΈΠΌ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΠΌ ΡΠΎΠΎΠ±ΡΠ΅ΡΡΠ²ΠΎΠΌ ΠΎΡΠ΅Π½Ρ ΡΠ»ΠΎΠΆΠ½ΡΡ Π·Π°Π΄Π°ΡΡ: ΡΠ΄Π²ΠΎΠΈΡΡ Π·Π° 3 Π³ΠΎΠ΄Π° ΡΠΈΡΠ»ΠΎ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΉ, ΠΈΠ½Π΄Π΅ΠΊΡΠΈΡΡΠ΅ΠΌΡΡ
Π² Web of Science. Π Π°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΠΏΠ΅ΡΠ²ΠΎΠΎΡΠ΅ΡΠ΅Π΄Π½ΡΠ΅ ΠΈ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΡΠ΅ ΠΌΠ΅ΡΡ Π΄Π»Ρ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΡΡΠΎΠΉ Π·Π°Π΄Π°ΡΠΈ.
Signatures of the excitonic memory effects in four-wave mixing processes in cavity polaritons
We report the signatures of the exciton correlation effects with finite
memory time in frequency domain degenerate four-wave mixing (DFWM) in
semiconductor microcavity. By utilizing the polarization selection rules, we
discriminate instantaneous, mean field interactions between excitons with the
same spins, long-living correlation due to the formation of biexciton state by
excitons with opposite spins, and short-memory correlation effects in the
continuum of unbound two-exciton states. The DFWM spectra give us the relative
contributions of these effects and the upper limit for the time of the
exciton-exciton correlation in the unbound two-exciton continuum. The obtained
results reveal the basis of the cavity polariton scattering model for the DFWM
processes in high-Q GaAs microcavity.Comment: 11 pages, 1 figur
ΠΠΠΠΠΠ ΠΠ£ΠΠΠΠΠΠ¦ΠΠΠΠΠΠΠ ΠΠΠ’ΠΠΠ Π ΠΠΠ ΠΠ 2011 Π. Π ΠΠΠ Π‘ΠΠΠΠ’ΠΠΠ« Π£ΠΠΠΠΠ§ΠΠΠΠ― ΠΠΠ ΠΠΠͺΠΠΠΠ Π ΠΠΠ’ΠΠ ΠΠ’ΠΠ’ΠΠΠ‘Π’Π Π Π‘ΠΠΠ’ΠΠΠ’Π‘Π’ΠΠΠ Π‘ ΠΠΠΠΠΠΠ’ΠΠ ΠΠΠ Β«ΠΠ ΠΠΠ ΠΠΠΠ« Π€Π£ΠΠΠΠΠΠΠ’ΠΠΠ¬ΠΠ«Π₯ ΠΠΠ£Π§ΠΠ«Π₯ ΠΠ‘Π‘ΠΠΠΠΠΠΠΠΠ ΠΠΠ‘ΠΠΠΠΠΠΠΠ ΠΠ 2013β2020 ΠΠ.Β»
The scope of bibliometric research is focused on academic output of the Russian Academy of Medical Sciences (RAMS) in 2011 by analyzing the data extracted from the Web of Science database and InCites application Research Performance Profile of RAMS. Using these analytical tools trends in dynamics of publications and citing of the RAMS researchers were calculated and the level of scientific output in some biomedical subject areas was estimated. Studies revealed the great importance of international collaboration and international coauthorship for RAMS in producing papers published in established scholarly journals. Basic metrics of scientific performance that should be required to achieve the goals declared in the recent governmental policy papers were measured for RAMS institutions as well. Another problem investigated in this research is the problem of low citedness of RAMS journal papers indexed in WoS. Factors and reasons influenced on low citedness were discovered In conclusion authors set out priorities and reasonable goal-oriented efforts to achieve better results in publication activity.Β Π ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΎ Π±ΠΈΠ±Π»ΠΈΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΠΊΠ° ΡΡΠ΅Π½ΡΡ
Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠΉ Π°ΠΊΠ°Π΄Π΅ΠΌΠΈΠΈ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΡ
Π½Π°ΡΠΊ (Π ΠΠΠ) Π² ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΠΎΠΉ Π°Π½Π°Π»ΠΈΡΠΈΠΊΠΎ-Π±ΠΈΠ±Π»ΠΈΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠ΅ Web of Science Π·Π° 2011Β Π³. Π ΡΠ°ΠΌΠΊΠ°Ρ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ» ΠΈΠ·ΡΡΠ΅Π½ ΠΏΡΠΎΡΠΈΠ»Ρ Π½Π°ΡΡΠ½ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π ΠΠΠ Π² Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΏΡΠΈΠ»ΠΎΠΆΠ΅Π½ΠΈΠΈ InCites. ΠΡΠΏΠΎΠ»Π½Π΅Π½ Π°Π½Π°Π»ΠΈΠ· Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈ ΡΠΈΡΠΈΡΡΠ΅ΠΌΠΎΡΡΠΈ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΉ ΡΠΎΡΡΡΠ΄Π½ΠΈΠΊΠΎΠ² ΠΈΠ½ΡΡΠΈΡΡΡΠΎΠ² Π ΠΠΠ, ΠΎΡΠ΅Π½Π΅Π½ ΡΡΠΎΠ²Π΅Π½Ρ Π½Π°ΡΡΠ½ΠΎΠΉ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΡΠΊΠΈΡ
Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠΉ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΠΎΠ³ΠΎ Π½Π°ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΡΠ΄Π½ΠΈΡΠ΅ΡΡΠ²Π°. ΠΠ°Π½Π° ΠΎΡΠ΅Π½ΠΊΠ° ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΡ ΡΠ΅Π°Π»ΡΠ½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΈΠ²Π½ΠΎΡΡΠΈ Π½Π°ΡΡΠ½ΠΎΠΉ Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ Π ΠΠΠ ΠΏΡΠΎΠ³Π½ΠΎΠ·Π½ΡΠΌ Π·Π½Π°ΡΠ΅Π½ΠΈΡΠΌ ΠΈΠ½Π΄ΠΈΠΊΠ°ΡΠΎΡΠΎΠ² ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, Π·Π°ΡΠ²Π»Π΅Π½Π½ΡΠΌ Π² ΡΡΠ΄Π΅ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ½ΡΡ
ΠΏΡΠ°Π²ΠΈΡΠ΅Π»ΡΡΡΠ²Π΅Π½Π½ΡΡ
Π΄ΠΎΠΊΡΠΌΠ΅Π½ΡΠΎΠ². ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π° ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ° ΠΎΠ±ΡΠ΅ΠΉ Π½ΠΈΠ·ΠΊΠΎΠΉ ΡΠΈΡΠΈΡΡΠ΅ΠΌΠΎΡΡΠΈ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΉ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»Π΅ΠΉ ΠΈΠ· Π ΠΠΠ ΠΈ ΠΎΡΡΡΠ΅ΡΡΠ²Π»Π΅Π½Π½ Π°Π½Π°Π»ΠΈΠ· ΡΠ°ΠΊΡΠΎΡΠΎΠ², Π²Π»ΠΈΡΡΡΠΈΡ
Π½Π° ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΈΡΠΈΡΡΠ΅ΠΌΠΎΡΡΠΈ ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠ°Π±ΠΎΡ Π² ΠΎΠ±Π»Π°ΡΡΠΈ Π±ΠΈΠΎΠΌΠ΅Π΄ΠΈΡΠΈΠ½Ρ. Π Π·Π°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠΈ Π΄Π°ΡΡΡΡ ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄Π°ΡΠΈΠΈ ΠΏΠΎ ΡΠΎΡΡΡ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΠΈ ΡΠΈΡΠ°ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π΄Π»Ρ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΉ Π ΠΠΠ.Β
-Strands
A -strand is a map for a Lie
group that follows from Hamilton's principle for a certain class of
-invariant Lagrangians. The SO(3)-strand is the -strand version of the
rigid body equation and it may be regarded physically as a continuous spin
chain. Here, -strand dynamics for ellipsoidal rotations is derived as
an Euler-Poincar\'e system for a certain class of variations and recast as a
Lie-Poisson system for coadjoint flow with the same Hamiltonian structure as
for a perfect complex fluid. For a special Hamiltonian, the -strand is
mapped into a completely integrable generalization of the classical chiral
model for the SO(3)-strand. Analogous results are obtained for the
-strand. The -strand is the -strand version of the
Bloch-Iserles ordinary differential equation, whose solutions exhibit dynamical
sorting. Numerical solutions show nonlinear interactions of coherent wave-like
solutions in both cases. -strand equations on the
diffeomorphism group are also introduced and shown
to admit solutions with singular support (e.g., peakons).Comment: 35 pages, 5 figures, 3rd version. To appear in J Nonlin Sc
Non-Kaehler Heterotic String Compactifications with non-zero fluxes and constant dilaton
We construct new explicit compact supersymmetric valid solutions with
non-zero field strength, non-flat instanton and constant dilaton to the
heterotic equations of motion in dimension six. We present balanced Hermitian
structures on compact nilmanifolds in dimension six satisfying the heterotic
supersymmetry equations with non-zero flux, non-flat instanton and constant
dilaton which obey the three-form Bianchi identity with curvature term taken
with respect to either the Levi-Civita, the (+)-connection or the Chern
connection. Among them, all our solutions with respect to the (+)-connection on
the compact nilmanifold satisfy the heterotic equations of motion.Comment: LaTeX, 16 pp., no figures, new Theorem 1.1, references adde
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