41 research outputs found
КОНВЕКТИВНЫЕ МЕТОДИКИ В ПРАКТИКЕ ПРОГРАММНОГО ГЕМОДИАЛИЗА ФНЦ ТРАНСПЛАНТОЛОГИИ И ИСКУССТВЕННЫХ ОРГАНОВ ИМЕНИ АКАДЕМИКА В.И. ШУМАКОВА: ИСТОРИЯ И СОВРЕМЕННОСТЬ
The experience of the introduction of convective methods since the eighties of the 20th century is described.Aim: to evaluate the infl uence of convective transport supplementation on renal replacement therapy results. Methods and results. The number of convective procedures was applied: hemodiafi ltration with commercial substituted fl uid, paired fi ltration dialysis, acetate-free biofi ltration, original hybrid methods, online hemodiafi ltration. Themain trend was the magnifi cation of substituted volume. In the long run after switching from conventional dialysis to convective therapy the increase of Kt/V, the decline of phosphate, the rise of serum albumin, the decrease of CRP level and ESA consumption, QOL improvement and the drop in severity of intradialysis complications were observed. Over last six years online hemodiafi ltration has become the standard of dialysis therapy in our Center.Conclusion. The undoubted advantages of convective modalities and accessibility of online hemodiafi ltration make these methods the gold standard of dialysis therapy.Описан опыт внедрения в практику программного гемодиализа конвективных методов с 80-х годов про-шлого столетия по настоящее время.Цель: оценить влияние конвективного переноса, прежде всего в рамках гемодиафильтрации «онлайн», на результаты заместительной терапии.Методы и результаты.Применены различные конвективные методики: гемодиафильтрация с использованием коммерческого замещающего раствора, парные фильтрация-диализ, безацетатная биофильтрация, оригинальные гибридные методики, гемодиафильтрация «онлайн». Основной тенденцией при этом было увеличение объема замещения за процедуру. При наблюдении в длительной перспективе после перевода со стандартного гемодиализа при использовании конвективных методик отмечалось увеличение эффективности лечения по индексу Kt/V, снижение фосфатемии, повышение концентрации альбумина, уменьшение уровня С-реактивного белка и потребности в эритропоэзтимулирующих препаратах, повышение качества жизни и снижение выраженности интрадиализной симптоматики. В течение последних 6 лет гемодиафильтрация «онлайн» стала стандартом диализного лечения в нашем учреждении.Заключение. Несомненные преимущества конвективных методик и доступность практически неограниченного объема замещающей жидкости, характеризующая гемодиафильтрацию «онлайн», делают этот метод «золотым» стандартом диализной терапии
Hierarchical structure of cascade of primary and secondary periodicities in Fourier power spectrum of alphoid higher order repeats
<p>Abstract</p> <p>Background</p> <p>Identification of approximate tandem repeats is an important task of broad significance and still remains a challenging problem of computational genomics. Often there is no single best approach to periodicity detection and a combination of different methods may improve the prediction accuracy. Discrete Fourier transform (DFT) has been extensively used to study primary periodicities in DNA sequences. Here we investigate the application of DFT method to identify and study alphoid higher order repeats.</p> <p>Results</p> <p>We used method based on DFT with mapping of symbolic into numerical sequence to identify and study alphoid higher order repeats (HOR). For HORs the power spectrum shows equidistant frequency pattern, with characteristic two-level hierarchical organization as signature of HOR. Our case study was the 16 mer HOR tandem in AC017075.8 from human chromosome 7. Very long array of equidistant peaks at multiple frequencies (more than a thousand higher harmonics) is based on fundamental frequency of 16 mer HOR. Pronounced subset of equidistant peaks is based on multiples of the fundamental HOR frequency (multiplication factor <it>n </it>for <it>n</it>mer) and higher harmonics. In general, <it>n</it>mer HOR-pattern contains equidistant secondary periodicity peaks, having a pronounced subset of equidistant primary periodicity peaks. This hierarchical pattern as signature for HOR detection is robust with respect to monomer insertions and deletions, random sequence insertions etc. For a monomeric alphoid sequence only primary periodicity peaks are present. The 1/<it>f</it><sup><it>β </it></sup>– noise and periodicity three pattern are missing from power spectra in alphoid regions, in accordance with expectations.</p> <p>Conclusion</p> <p>DFT provides a robust detection method for higher order periodicity. Easily recognizable HOR power spectrum is characterized by hierarchical two-level equidistant pattern: higher harmonics of the fundamental HOR-frequency (secondary periodicity) and a subset of pronounced peaks corresponding to constituent monomers (primary periodicity). The number of lower frequency peaks (secondary periodicity) below the frequency of the first primary periodicity peak reveals the size of <it>n</it>mer HOR, i.e., the number <it>n </it>of monomers contained in consensus HOR.</p
Source of polarised deuterons
The proposed project assumes the development of a universal high-intensity source of polarized deuterons (protons) using a charge-exchange
plasma ionizer. The design output current of the source will be up to 10 mA
for and polarization will be up
to of the maximal vector () and tensor (+1,-2) polarization.
The project is based on the equipment which was supplied within the framework
of an agreement between JINR and IUCF (Bloomington, USA). The project will be
realized in close cooperation with INR (Moscow, Russia). The source will be
installed in the linac hall (LU-20) and polarization of beams will be measured
at the output of LU-20. The main purpose of the project is to increase the
intensity of the accelerated polarized beams at the JINR Accelerator Complex up
to 1010 d/pulse. Calculations and first accelerator runs have shown that
the depolarization resonances are absent for the deuteron beam in the entire
energy range of the NUCLOTRON. The source could be transformed into a source of
polarized negative ions if necessary. The period of reliable operation without
participation of the personnel should be within 1000 hours. The project should
be implemented within two to two and a half years from the start of funding
CONVECTIVE METHODS IN CLINICAL PRACTICE OF V.I. SHUMAKOV FEDERAL RESEARCH CENTER OF TRANSPLANTOLOGY AND ARTIFICIAL ORGANS: PAST AND PRESENT
The experience of the introduction of convective methods since the eighties of the 20th century is described.Aim: to evaluate the infl uence of convective transport supplementation on renal replacement therapy results. Methods and results. The number of convective procedures was applied: hemodiafi ltration with commercial substituted fl uid, paired fi ltration dialysis, acetate-free biofi ltration, original hybrid methods, online hemodiafi ltration. Themain trend was the magnifi cation of substituted volume. In the long run after switching from conventional dialysis to convective therapy the increase of Kt/V, the decline of phosphate, the rise of serum albumin, the decrease of CRP level and ESA consumption, QOL improvement and the drop in severity of intradialysis complications were observed. Over last six years online hemodiafi ltration has become the standard of dialysis therapy in our Center.Conclusion. The undoubted advantages of convective modalities and accessibility of online hemodiafi ltration make these methods the gold standard of dialysis therapy