A blind decision feedback equalizer incorporating fixed lag smoothing

Copyright © 2000 IEEEA new type of blind decision feedback equalizer (DFE) incorporating fixed lag smoothing is developed in this paper. The structure is motivated by the fact that if we make full use of the dependence of the observed data on a given transmitted symbol, delayed decisions may produce better estimates of that symbol. To this end, we use a hidden Markov model (HMM) suboptimal formulation that offers a good tradeoff between computational complexity and bit error rate (BER) performance. The proposed equalizer also provides estimates of the channel coefficients and operates adaptively (so that it can adapt to a fading channel for instance) by means of an online version of the expectation-maximization (EM) algorithm. The resulting equalizer structure takes the form of a linear feedback system including a quantizer, and hence, it is easily implemented. In fact, because of its feedback structure, the proposed equalizer shows some similarities with the well-known DFE. A full theoretical analysis of the initial version of the algorithm is not available, but a characterization of a simplified version is provided. We demonstrate that compared to the zero-forcing DFE (ZF-DFE), the algorithm yields many improvements. A large range of simulations on finite impulse response (FIR) channels and on typical fading GSM channel models illustrate the potential of the proposed equalizer.Sylvie Perreau, Langford B. White and Pierre Duhame

Tree indexed Markov processes and long range dependency

Langford B. White and Sylvie L. Perrea

The Control Of Zealactone Biosynthesis And Exudation Is Involved In The Response To Nitrogen In Maize Root.

Nitrate acts as a signal in regulating plant development in response to environment. In particular nitric oxide (NO), auxin and strigolactones (SLs) were supposed to cooperate to regulate the maize root response to this anion. In this study, a combined approach based on LC-MS/MS and on physiological and molecular analyses was adopted to specify the involvement of SLs in the maize response to N. Our results showed that N deficiency strongly induces SL exudation, likely through stimulating their biosynthesis. Nitrate provision early counteracts and also ammonium lowers SL exudation, but less markedly. Exudates obtained from N-starved and ammonium-provided seedlings stimulated Phelipanche germination, whereas when seeds were treated with exudates harvested from nitrate-provided plants no germination was observed. Furthermore, our findings support the idea that the inhibition of SL production observed in response to nitrate and ammonium would contribute to the regulation of lateral root development. Moreover, the transcriptional regulation of a gene encoding a putative maize WBC transporter, in response to various nitrogen supplies, together with its mRNA tissue localization, supported its role in SL allocation. Our results highlight the dual role of SLs as molecules able to signal outwards a nutritional need and as endogenous regulators of root architecture adjustments to N, thus synchronizing plant growth with nitrogen acquisitio

Types sérologiques de Pasteurella multocida rencontrés en France chez le lapin et la poule

Perreau Pierre, Renault Lucien, Vallée A. Types sérologiques de Pasteurella multocida rencontrés en France chez le lapin et la poule. In: Bulletin de l'Académie Vétérinaire de France tome 115 n°8, 1962. pp. 295-298

Effects of diet quality on morphology and intraspecific competition ability during development : the case of fire salamander larvae

The environmental conditions of the habitats in which organisms live may induce a variety of plastic adaptive responses for numerous developmental, behavioural and morphological traits. One of the most relevant environmental features is the amount of available food occurring in a certain habitat. In this study, we wanted to assess whether, during development of vertebrates, diet quality may induce plastic responses at both behavioural and morphological levels. We tested whether diet may affect the rate of aggressive interactions between fire salamander larvae (Salamandra salamandra) and changes of head morphology during development. We collected 15 newborn larvae from five different localities, and we randomly assigned them to three diets (small nourishing prey, big scant prey, mixed prey). For each larva we recorded the number of snap attempts to a target larva 4\ua0days after salamander collection, and after the 50\ua0days of feeding treatment. We recorded and analysed head shape development using a geometric morphometrics approach. Analyses showed a significant relationship between diet and aggressiveness: larvae fed with small nourishing prey were significantly more aggressive. Diet had a significant effect in determining the quantity of head morphology changes during larval development, but did not affect the characteristics of the morphology; this means that all the larvae showed a similar shape modification, but those treated with the more nourishing prey showed a more pronounced change. These results indicate that diet features may induce both behavioural and morphological plastic responses. High-quality diets may be linked to competition for trophic resources, increasing development rates and determining higher competitive ability

Personalized Cytokine-Directed Therapy With Tocilizumab for Refractory Immune Checkpoint Inhibitor-Related Cholangiohepatitis.

For patients with corticosteroid (CS)-refractory immune checkpoint inhibitor-related cholangiohepatitis (irCH), no consensus exists regarding treatment, and outcomes remain poor. We evaluated the possibility of personalized treatment according to the patient's cytokine profile and the immunohistopathologic assessment of the predominant immune infiltrate type of liver tissue. NSCLCs with CS-refractory irCH were analyzed by immunohistochemistry of liver biopsy specimen, serum cytokine panel, and assessment of peripheral blood mononuclear cell immune cell monitoring by mass cytometry. A total of three consecutive patients with irCH were identified. We found a predominant T-cell infiltrate and an interferon-gamma or T helper 1 proinflammatory cytokine profile. Here, we report for the first time that a T-cell-targeted therapy with the interleukin (IL)-6 receptor-neutralizing antibody tocilizumab, which inhibits signaling downstream of interferon-gamma and several other Janus kinase-dependent cytokines, is an effective single cytokine-directed therapy for CS-refractory irCH. Three patients with severe, CS-refractory irCH who were treated with tocilizumab were found to have persistent clinical and biological remission. Dysregulation of the IL-6/T-cell axis may contribute to the pathogenesis of CS-refractory irCH. Our observations suggest that IL-6 blockade seems to have promise in the treatment of CS-refractory irCH. The results from our three patients need to be confirmed in a larger patient population

Active PD-L1 incorporation within HIV virions functionally impairs T follicular helper cells.

The limited development of broadly neutralizing antibodies (BnAbs) during HIV infection is classically attributed to an inadequate B-cell help brought by functionally impaired T follicular helper (Tfh) cells. However, the determinants of Tfh-cell functional impairment and the signals contributing to this condition remain elusive. In the present study, we showed that PD-L1 is incorporated within HIV virions through an active mechanism involving p17 HIV matrix protein. We subsequently showed that in vitro produced PD-L1high but not PD-L1low HIV virions, significantly reduced Tfh-cell proliferation and IL-21 production, ultimately leading to a decreased of IgG1 secretion from GC B cells. Interestingly, Tfh-cell functions were fully restored in presence of anti-PD-L1/2 blocking mAbs treatment, demonstrating that the incorporated PD-L1 proteins were functionally active. Taken together, the present study unveils an immunovirological mechanism by which HIV specifically exploits the regulatory potential of PD-L1 to suppress the immune system during the course of HIV infection

CD160-Associated CD8 T-Cell Functional Impairment Is Independent of PD-1 Expression.

Expression of co-inhibitory molecules is generally associated with T-cell dysfunction in chronic viral infections such as HIV or HCV. However, their relative contribution in the T-cell impairment remains unclear. In the present study, we have evaluated the impact of the expression of co-inhibitory molecules such as 2B4, PD-1 and CD160 on the functions of CD8 T-cells specific to influenza, EBV and CMV. We show that CD8 T-cell populations expressing CD160, but not PD-1, had reduced proliferation capacity and perforin expression, thus indicating that the functional impairment in CD160+ CD8 T cells may be independent of PD-1 expression. The blockade of CD160/CD160-ligand interaction restored CD8 T-cell proliferation capacity, and the extent of restoration directly correlated with the ex vivo proportion of CD160+ CD8 T cells suggesting that CD160 negatively regulates TCR-mediated signaling. Furthermore, CD160 expression was not up-regulated upon T-cell activation or proliferation as compared to PD-1. Taken together, these results provide evidence that CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression