Addiction-related genes in gambling disorders:new insights from parallel human and pre-clinical models

Neurobiological research supports the characterization of disordered gambling (DG) as a behavioral addiction. Recently, an animal model of gambling behavior was developed (rat gambling task, rGT), expanding the available tools to investigate DG neurobiology. We investigated whether rGT performance and associated risk gene expression in the rat's brain could provide cross-translational understanding of the neuromolecular mechanisms of addiction in DG. We genotyped tagSNPs (single-nucleotide polymorphisms) in 38 addiction-related genes in 400 DG and 345 non-DG subjects. Genes with P<0.1 in the human association analyses were selected to be investigated in the animal arm to determine whether their mRNA expression in rats was associated with the rat's performance on the rGT. In humans, DG was significantly associated with tagSNPs in DRD3 (rs167771) and CAMK2D (rs3815072). Our results suggest that age and gender might moderate the association between CAMK2D and DG. Moderation effects could not be investigated due to sample power. In the animal arm, only the association between rGT performance and Drd3 expression remained significant after Bonferroni correction for 59 brain regions. As male rats were used, gender effects could not be investigated. Our results corroborate previous findings reporting the involvement of DRD3 receptor in addictions. To our knowledge, the use of human genetics, pre-clinical models and gene expression as a cross-translation paradigm has not previously been attempted in the field of addictions. The cross-validation of human findings in animal models is crucial for improving the translation of basic research into clinical treatments, which could accelerate neurobiological and pharmacological investigations in addictions

Folded Three-Spin String Solutions in AdS_5 x S^5

We construct a spinning closed string solution in AdS_5 x S^5 which is folded in the radial direction and has two equal spins in AdS_5 and a spin in S^5. The energy expression of the three-spin solution specified by the folding and winding numbers for the small S^5 spin shows a logarithmic behavior and a one-third power behavior of the large total AdS_5 spin, in the long string and in the short string located near the boundary of AdS_5 respectively. It exhibits the non-regular expansion in the 't Hooft coupling constant, while it takes the regular one when the S^5 spin becomes large.Comment: 14 pages, LaTeX, no figures, a reference adde

PREVENTION OF ONCOLOGICAL DISEASES AS THE BASIS OF INTERACTION OF ONCOLOGICAL SERVICE WITH PRIMARY LINK OF HEALTH CARE

Malignant neoplasms (MN) are the second cause of death for the population of Russia, resulted in 15.5% of all deaths in 2015. Their share in the structure of the male population mortality rate was 16.4%, female — 14.4%. Among both sexes, deaths of working age, the proportion of deaths from MN was 16.0%, among women of reproductive age — 16.3%. More than half of the working age population of deaths in Russia falls on the disease from the group of preventable death, and a third — to preventable causes, depending on the primary and secondary prevention, quality of care, along with a high prevalence of behavioral risk factors (RF), demographic characteristics, marked medical and social reasons for the high mortality rate of the MN: late uptake of the population for health care, lack of alertness in oncology healthcare professionals, patients defects in routing.The leading factor in increasing life expectancy is prevention. Today, prevention is regarded as active method of strengthening and preservation of health, and the currently existing approaches in promoting healthy life mostly only directed at the prevention of behavioral risk factors: tobacco use, poor diet, physical inactivity and harmful use of alcohol. Experts estimate the impact on them will prevent, at least 40% of the cases of MN.In Russia we have a 3-step system of health care. In general preventive health orientation is implemented on the 1st level: in the provision of primary health care. In this regard, particular attention should be paid to the implementation of measures for specific clinical examination of the adult population, which plays an important role in the early detection of both the disease and the risk factors for their development. Properly organized conduct of the clinical examination can provide a substantial, up to 30%, the contribution to the reduction of total mortality, including mortality from MN. The necessity of increasing the role of the oncology service in the organizational and methodological support of the preventive measures implemented by primary health care is performed

Spinning Pulsating String Solitons in AdS_5 x S^5

We point out the existence of some simple string solitons in AdS_5 x S^5, which at the same time are spinning in AdS_5 and pulsating in S^5, or vice-versa. This introduces an additional arbitrary constant into the scaling relations between energy and spin or R-charge. The arbitrary constant is not an angular momentum, but can be related to the amplitude of the pulsation. We discuss the solutions in detail and consider the scaling relations. Pulsating multi spin or multi R-charge solutions can also be constructed.Comment: 15 pages, Late

Long-term results of surgical correction of Scheuermann’s kyphosis

Objective. To analyze long-term results of surgical correction of kyphosis due to Scheuermann’s disease. Material and Methods. Design: retrospective cohort study. The study group included 43 patients (m/f ratio, 34/9). The mean age was 19.1 (14–32) years; the mean postoperative follow-up was 6 ± 10 (5–20) years. Two-stage surgery including discectomy and interbody fusion followed by posterior correction and fusion was conducted in 35 cases (Group A). Eight patients (Group B) underwent only posterior correction and spinal fusion. The following parameters were determined for each patient: thoracic kyphosis (TK), lumbar lordosis (LL) (scoliotic deformity of the thoracic/thoracolumbar spine, if the curve magnitude was > 5°), sagittal vertical axis (SVA), sagittal stable vertebra (SSV), first lordotic vertebra (FLV), proximal junctional angle (PJA) and distal junctional angle (DJA). All measurements were performed immediately before surgery, one week after surgery, and at the end of the follow-up period. All patients answered the SRS-24 questionnaire after surgery and at end of the follow-up period. Results. Groups were comparable in terms of age and gender of patients, body mass index and initial Cobb angle (p < 0.05). The curve decreased from 77.8° to 40.7° in Group A and from 81.7° to 41.6° in Group B. The loss of correction was 9.1° and 6.0° in groups A and B, respectively. The parameters of lumbar lordosis remained normal during the follow-up period. At implant density less than 1.2, deformity correction and correction loss were 44.5° (54.7 %) and 3.9°, respectively (p < 0.05). Proximal junctional kyphosis (PJK) was detected in 21 out of 43 patients (48.8 %). The frequency of PJK was 45.4 % among patients whose upper end vertebra was included in the fusion and 60 % among those whose upper end vertebra was not included. PJK developed in eight (47.8 %) out of 17 patients with kyphosis correction ≥ 50 % and in 13 (50.0 %) of those with correction < 50 %. The rate of DJK development was 39.5 %. The lower instrumented vertebra (LIV) was located proximal to the sagittal stable vertebra in 16 cases, with 12 (75 %) of them being diagnosed with DJK. In 27 patients, LIV was located either at the SSV level or distal to it, the number of DJK cases was 5 (18.5 %); p < 0.05. Only two patients with complications required unplanned interventions. According to the patient questionnaires, the surgical outcome score increases between the immediate and long-term postoperative periods for all domains and from 88.4 to 91.4 in total. The same applies to answer to the question about consent to surgical treatment on the same conditions: positive answers increased from 82 to 86 %. Conclusions. Two-stage surgery, as a more difficult and prolonged one, has no advantages over one-stage operation in terms of correction magnitude and stability of the achieved effect. Surgical treatment improves the quality of life of patients with Scheuermann’s disease, and the improvement continues in the long-term postoperative period

Novel merwinite/akermanite ceramics: in vitro bioactivity

The ceramics in the system CaO – MgO – SiO2 has recently received a great deal of attention because they exhibit good in vitro bioactivity and have potential use as bone implants. Biphasic calcium-magnesium-silicate ceramics was prepared by a sol-gel method. The dried gel with chemical composition 3CaO.MgO.2SiO2 was thermally treated at 1300°C for 2 h. The structural behavior of the synthesized ceramics was examined by means of X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Merwinite, as the main crystalline phase, and akermanite, as the minor phase, were identified. The in vitro bioactivity of the synthesized ceramic samples was recorded in Simulated Body Fluid (SBF) for different times of soaking. The apatite formation on the surface of the immersed samples was detected by FTIR, SEM and Energy Dispersive Spectroscopy (EDS) techniques. The ion concentrations in the SBF solutions after the in vitro test were evaluated by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). On the basis of the results obtained, the ability of the biphasic ceramics to deposit apatite layer was found. The peculiarities of the formation of apatite layer depending on the phase composition were analyzed and discussed