548 research outputs found
Intra- and Inter-Day Variability in Recess Physical Activity Over One Week in Young Children
Researchers interested in outdoor free play (i.e., recess) may collect data on total (light-to-vigorous) physical activity (TPA) during a single recess period or over several days, yet little is known about intra- and inter-day variability or whether individual recess periods or days are representative of “typical” recess TPA. PURPOSE: The purpose of the current study was to explore variability in TPA across a week of recess periods with the overarching goal of identifying how many recess periods may be needed to represent weekly recess TPA. METHODS: Children (n=29; age=2-6 y) from two on-campus early childhood education programs which shared a schoolyard wore a triaxial GENEActiv accelerometer at the right hip during school hours for a week. Euclidean norm minus one per 5-sec was calculated and used to estimate percent of recess time spent in TPA. A mixed effects model compared TPA by day and recess time of day (fixed effects) with child as a random effect. We averaged TPA for each of the 13 individual recesses, scheduled recess time (morning, afternoon, end of day), and day (Monday-Friday). T-tests were used to compare TPA during individual recess periods, scheduled recess times, and days to overall average weekly recess TPA. P-values were adjusted for multiple comparisons using the Bonferroni correction.RESULTS: Overall weekly recess TPA was 62.3±9.7%. TPA ranged from 47.3-82.5% across the 13 individual recess periods, 56.0-63.1% across scheduled recess times, and 54.0-69.2% across days. In the mixed effects model, there was a significant effect of day, but not scheduled recess time. Post-hoc comparisons indicated Friday TPA was different from Monday (p=0.006), Tuesday (pCONCLUSIONS: Although an individual recess period is insufficient, capturing recess PA over a single day or a single time of day (e.g., morning recess for a week) is representative of weekly recess TPA at the group level, although TPA may vary by day for individuals. This could be a less burdensome and more efficient method of characterizing recess TPA when informing and assessing interventions
Movement of Smallmouth Bass within the Beaver Island Archipelago, Northern Lake Michigan.
Fish movement may vary across a wide array of aquatic ecosystems and may be related to the overall size of the system inhabited. We investigated movement of smallmouth bass in Lake Michigan because this information is lacking for larger systems. A total of 16 smallmouth bass were surgically implanted with ultrasonic transmitters within the Beaver Archipelago, northern Lake Michigan. During 2007–2008, a maximum of one location per individual was recorded daily during three specific tracking periods – pre-spawn, spawning, and post-spawn – to determine diurnal movement patterns. Movement was evaluated as site fidelity, minimum displacement rate,maximumexcursion rate, and distance from shore. Smallmouth bass exhibited greatermaximum excursion rates during the spawn period compared to pre-spawn. Movement rates did not differ between tracking periods; however, movement rates were greater during the spawn period in 2007 than 2008. Both sexes moved further offshore to deeper water during post-spawn, but females were located further offshore than males during this period. Annual site fidelity was more evident during post-spawn than during spawning for both sexes. Two smallmouth bass emigrated outside of the Archipelago, suggesting this population may be more “open” in terms of individuals moving throughout northern Lake Michigan than previously thought. These results indicate smallmouth bass may move greater distances in larger aquatic systems and therefore larger management units (in terms of total area) should be established in Lake Michigan to account for these greater excursion distances
Movement of Smallmouth Bass within the Beaver Island Archipelago, Northern Lake Michigan.
Fish movement may vary across a wide array of aquatic ecosystems and may be related to the overall size of the system inhabited. We investigated movement of smallmouth bass in Lake Michigan because this information is lacking for larger systems. A total of 16 smallmouth bass were surgically implanted with ultrasonic transmitters within the Beaver Archipelago, northern Lake Michigan. During 2007–2008, a maximum of one location per individual was recorded daily during three specific tracking periods – pre-spawn, spawning, and post-spawn – to determine diurnal movement patterns. Movement was evaluated as site fidelity, minimum displacement rate,maximumexcursion rate, and distance from shore. Smallmouth bass exhibited greatermaximum excursion rates during the spawn period compared to pre-spawn. Movement rates did not differ between tracking periods; however, movement rates were greater during the spawn period in 2007 than 2008. Both sexes moved further offshore to deeper water during post-spawn, but females were located further offshore than males during this period. Annual site fidelity was more evident during post-spawn than during spawning for both sexes. Two smallmouth bass emigrated outside of the Archipelago, suggesting this population may be more “open” in terms of individuals moving throughout northern Lake Michigan than previously thought. These results indicate smallmouth bass may move greater distances in larger aquatic systems and therefore larger management units (in terms of total area) should be established in Lake Michigan to account for these greater excursion distances
Fish Assemblage Shifts and Population Dynamics of Smallmouth Bass (Micropterus dolomieu) in the Beaver Archipelago, Northern Lake Michigan: A Comparison Between Historical and Recent Time Periods Amidst Ecosystem Changes.
The ecological and economic importance of Great Lakes nearshore areas and the paucity of information on nearshore Lake Michigan fish assemblages prompted us to document changes that occurred from a historical time period (1969–1972, 1975, 1977, and 1984) to a recent period (2005–2008) in a nearshore northern Lake Michigan (Beaver Archipelago) fish assemblage, with an emphasis on smallmouth bass Micropterus dolomieu. From historical to recent periods, the Beaver Archipelago fish assemblage shifted from predominantly brown bullheads Ameiurus nebulosus to predominantly smallmouth bass. Relative abundance of brown bullheads and white suckers Catostomus commersonii declined from historical to recent time periods, as did overall species richness. The relative abundance, recruitment variability, and mortality rates of smallmouth bass have not significantly changed since the historical time period, whereas both condition (ages 5–7) and growth (ages 2–7) of this species have significantly increased. Our results suggest that the smallmouth bass population in the Beaver Archipelago area has not been negatively affected by recent ecological changes (i.e., declining primary productivity, increasing benthic invertebrate densities, increasing numbers of double-crested cormorants Phalacrocorax auritus, and increasing introductions of nonnative species). The smallmouth bass is currently the dominant nearshore species and remains a critical component of the nearshore fish assemblage in northern Lake Michigan
Monitoring wolf populations using howling points combined with sign survey transects
Wolves respond to simulated howling, especially during the mating and breeding seasons. Simulated howling points are, therefore, commonly used by many wolf researchers around the world to estimate pack numbers in a given area. A large amount of information is available on various pack breeding
areas in Asturias, the only region in north-western Spain where the Iberian wolf (Canis lupus signatus, Cabrera 1907) is not classed as a game species. Wolf research began there in the early 1980s. We present the results of the latest study on population status, conducted between July and November, 2001. Using sampling transects to detect wolf scat and scratch marks and designated howling and observation points, twenty one (21) wolf packs were
definitely located, with two others considered “likely”. Nineteen (19) packs were detected using howling points (n=314). The results of this study show that simulated howling points and sampling transects are reliable and
inexpensive way of detecting wolf packs
Nuclear localization and function of polypeptide ligands and their receptors: a new paradigm for hormone specificity within the mammary gland?
The specific effects triggered by polypeptide hormone/growth factor stimulation of mammary cells were considered mediated solely by receptor-associated signaling networks. A compelling body of new data, however, clearly indicates that polypeptide ligands and/or their receptors are transported into the nucleus, where they function directly to regulate the expression of specific transcription factors and gene loci. The intranuclear function of these complexes may contribute to the explicit functions associated with a given ligand, and may serve as new targets for pharmacologic intervention
Prolactin receptor is a negative prognostic factor in patients with squamous cell carcinoma of the head and neck
Background:
The influence of human prolactin (hPRL) on the development of breast and other types of cancer is well established. Little information, however, exists on the effects of hPRL on squamous cell carcinomas of the head and neck (SCCHNs).
Methods:
In this study, we evaluated prolactin receptor (PRLR) expression in SCCHN cell lines and assessed by immunohistochemistry the expression in 89 patients with SCCHNs. The PRLR expression was correlated with clinicopathological characteristics as well as clinical outcome. The effect of hPRL treatment on tumour cell growth was evaluated in vitro.
Results:
Immunoreactivity for PRLR was observed in 85 out of 89 (95%) tumours. Multivariate COX regression analysis confirmed high levels of PRLR expression (>25% of tumour cells) to be an independent prognostic factor with respect to overall survival (HR=3.70, 95% CI: 1.14–12.01; P=0.029) and disease-free survival (P=0.017). Growth of PRLR-positive cancer cells increased in response to hPRL treatment.
Conclusion:
Our data indicate that hPRL is an important growth factor for SCCHN. Because of PRLR expression in a vast majority of tumour specimens and its negative impact on overall survival, the receptor represents a novel prognosticator and a promising drug target for patients with SCCHNs
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High-resolution spatial and temporal measurements of particulate organic carbon flux using thorium-234 in the northeast Pacific Ocean during the EXport processes in the ocean from RemoTe sensing field campaign
© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Buesseler, K. O., Benitez-Nelson, C. R., Roca-Marti, M., Wyatt, A. M., Resplandy, L., Clevenger, S. J., Drysdale, J. A., Estapa, M. L., Pike, S., & Umhau, B. P. High-resolution spatial and temporal measurements of particulate organic carbon flux using thorium-234 in the northeast Pacific Ocean during the EXport processes in the ocean from RemoTe sensing field campaign. Elementa: Science of the Anthropocene, 8(1), (2020): 030, https://doi.org/10.1525/elementa.030.The EXport Processes in the Ocean from RemoTe Sensing (EXPORTS) program of National Aeronautics and Space Administration focuses on linking remotely sensed properties from satellites to the mechanisms that control the transfer of carbon from surface waters to depth. Here, the naturally occurring radionuclide thorium-234 was used as a tracer of sinking particle flux. More than 950 234Th measurements were made during August–September 2018 at Ocean Station Papa in the northeast Pacific Ocean. High-resolution vertical sampling enabled observations of the spatial and temporal evolution of particle flux in Lagrangian fashion. Thorium-234 profiles were remarkably consistent, with steady-state (SS) 234Th fluxes reaching 1,450 ± 300 dpm m−2 d−1 at 100 m. Nonetheless, 234Th increased by 6%–10% in the upper 60 m during the cruise, leading to consideration of a non-steady-state (NSS) model and/or horizontal transport, with NSS having the largest impact by decreasing SS 234Th fluxes by 30%. Below 100 m, NSS and SS models overlapped. Particulate organic carbon (POC)/234Th ratios decreased with depth in small (1–5 μm) and mid-sized (5–51 μm) particles, while large particle (>51 μm) ratios remained relatively constant, likely influenced by swimmer contamination. Using an average SS and NSS 234Th flux and the POC/234Th ratio of mid-sized particles, we determined a best estimate of POC flux. Maximum POC flux was 5.5 ± 1.7 mmol C m−2 d−1 at 50 m, decreasing by 70% at the base of the primary production zone (117 m). These results support earlier studies that this site is characterized by a modest biological carbon pump, with an export efficiency of 13% ± 5% (POC flux/net primary production at 120 m) and 39% flux attenuation in the subsequent 100 m (POC flux 220 m/POC flux 120m). This work sets the foundation for understanding controls on the biological carbon pump during this EXPORTS campaign.The authors would like to acknowledge support from the National Aeronautics and Space Administration (NASA) as part of the EXport Processes in the Ocean from RemoTe Sensing (EXPORTS) program awards 80NSSC17K0555 and 80NSSC17K0662; the Woods Hole Oceanographic Institution’s Ocean Twilight Zone study for KOB and MRM, and the National Science Foundation Graduate Research Fellowship Program (NSF-GRFP) for funding and support of AW
The relationship between city “greenness” and homicide in the US : evidence over a 30-year period
Residents in US cities are exposed to high levels of stress and violent crime. At the same time, a number of cities have put forward “greening” efforts which may promote nature’s calming effects and reduce stressful stimuli. Previous research has shown that greening may lower aggressive behaviors and violent crime. In this study we examined, for the first time, the longitudinal effects over a 30-year period of average city greenness on homicide rates across 290 major cities in the US, using multilevel linear growth curve modeling. Overall, homicide rates in US cities decreased over this time-period (52.1–33.5 per 100,000 population) while the average greenness increased slightly (0.41–0.43 NDVI). Change in average city greenness was negatively associated with homicide, controlling for a range of variables (β = −.30, p-value = .02). The results of this study suggest that efforts to increase urban greenness may have small but significant violence-reduction benefits.https://journals.sagepub.com/home/EABhj2023Geography, Geoinformatics and Meteorolog
Context-dependent effects on spatial variation in deer-vehicle collisions
Identifying factors that contribute to the risk of wildlife‐vehicle collisions (WVCs) has been a key focus of wildlife managers, transportation safety planners and road ecologists for over three decades. Despite these efforts, few generalities have emerged which can help predict the occurrence of WVCs, heightening the uncertainty under which conservation, wildlife and transportation management decisions are made. Undermining this general understanding is the use of study area boundaries that are incongruent with major biophysical gradients, inconsistent data collection protocols among study areas and species‐specific interactions with roads. We tested the extent to which factors predicting the occurrence of deer‐vehicle collisions (DVCs) were general among five study areas distributed over a 11,400‐km2 region in the Canadian Rocky Mountains. In spite of our system‐wide focus on the same genus (i.e., Odocoileus hemionus and O. virginianus), study area delineation along major biophysical gradients, and use of consistent data collection protocols, we found that large‐scale biophysical processes influence the effect of localized factors. At the local scale, factors predicting WVC occurrence varied greatly between individual study areas. Distance to water was an important predictor of WVCs in three of the five study areas, while other variables had modest importance in only two of the five study areas. Thus, lack of generality in factors predicting WVCs may have less to do with methodological or taxonomic differences among study areas than the large‐scale, biophysical context within which the data were collected. These results highlight the critical need to develop a conceptual framework in road ecology that can unify the disparate results emerging from field studies on WVC occurrence
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