Proceedings of the 24th annual Central Plains irrigation conference

Presented at Proceedings of the 24th annual Central Plains irrigation conference held on February 21-22 in Colby, Kansas.Includes bibliographical references

Effects of Source on Copper Uptake by Swine

Four catheterized crossbred (Y x H) barrows were used in a 4 x 4 latin square arrangement of treatments in a completely randomized block design, to determine the effect of source on copper uptake as determined by serum Cu concentration changes over a 7.5-hr sampling period

Effects of Source on Copper Uptake by Swine

Four catheterized crossbred (Y x H) barrows were used in a 4 x 4 latin square arrangement of treatments in a completely randomized block design, to determine the effect of source on copper uptake as determined by serum Cu concentration changes over a 7.5-hr sampling period

Vaginal metastasis of a Ewing sarcoma five years after resection of the primary tumor

A 35-year-old female presented with pain and swelling of the distal left radius. A diagnosis of Ewing sarcoma was made and she underwent neoadjuvant chemotherapy and surgery. Macroscopic viable areas remained on the map of the surgical specimen; as such, she was classified as a poor responder and received high dose adjuvant chemotherapy. She remained disease-free for five years, until age 40. A vaginal polyp was then detected during a routine gynaecologic examination. It was removed and histopathology revealed metastatic Ewing sarcoma

Nebraska Agricultural Water Management Demonstration Network (NAWMDN): Integrating Research and Extension/Outreach

Maximizing the net benefits of irrigated plant production through appropriately designed agricultural water management programs is of growing importance in Nebraska, and other western and Midwestern states, because many areas are involved in management and policy changes to conserve irrigation water. In Nebraska, farmers are being challenged to practice conservation methods and use water resources more efficiently while meeting plant water requirements and maintaining high yields. Another challenge Nebraska experiences in it\u27s approximately 3.5‐million‐ha irrigated lands is limited adoption of newer technologies/tools to help farmers better manage irrigation, conserve water and energy, and increase plant water use efficiency. In 2005, the Nebraska Agricultural Water Management Demonstration Network (NAWMDN or Network) was formed from an interdisciplinary team of partners including the Natural Resources Districts (NRD); USDA‐NRCS; farmers from south central, northeast, west central, and western Nebraska; crop consultants; and University of Nebraska‐Lincoln faculty. The main goal of the Network is to enable the transfer of high quality research‐based information to Nebraskans through a series of demonstration projects established in farmers\u27 fields and implement newer tools and technologies to address and enhance plant water use efficiency, water conservation, and reduce energy consumption for irrigation. The demonstration projects are supported by the scientifically‐based field research and evaluation projects conducted at the University of Nebraska‐Lincoln, South Central Agricultural Laboratory located near Clay Center, Nebraska. The Network was formed with only 15 farmers as collaborators in only one of the 23 NRDs in 2005. As of late 2009, the number of active collaborators has increased to over 300 in 12 NRDs and 35 of 93 counties. The Network is impacting both water and energy conservation due to farmers adopting information and newer technologies for irrigation management. The NAWMDN is helping participants to improve irrigation management and efficiency by monitoring plant growth stages and development, soil moisture, and crop evapotranspiration. As a result, they are reducing irrigation water application amounts and associated energy savings is leading to greater profitability to participating farmers. This article describes the goals and objectives of the Network, technical and educational components, operational functions, and procedures used in the NAWMDN. The quantitative impacts in terms of water and energy conservation are reported

NEBRASKA AGRICULTURAL WATER MANAGEMENT DEMONSTRATION NETWORK (NAWMDN): INTEGRATING RESEARCH AND EXTENSION/OUTREACH

Maximizing the net benefits of irrigated plant production through appropriately designed agricultural water management programs is of growing importance in Nebraska, and other western and Midwestern states, because many areas are involved in management and policy changes to conserve irrigation water. In Nebraska, farmers are being challenged to practice conservation methods and use water resources more efficiently while meeting plant water requirements and maintaining high yields. Another challenge Nebraska experiences in it\u27s approximately 3.5‐million‐ha irrigated lands is limited adoption of newer technologies/tools to help farmers better manage irrigation, conserve water and energy, and increase plant water use efficiency. In 2005, the Nebraska Agricultural Water Management Demonstration Network (NAWMDN or Network) was formed from an interdisciplinary team of partners including the Natural Resources Districts (NRD); USDA‐NRCS; farmers from south central, northeast, west central, and western Nebraska; crop consultants; and University of Nebraska‐Lincoln faculty. The main goal of the Network is to enable the transfer of high quality research‐based information to Nebraskans through a series of demonstration projects established in farmers\u27 fields and implement newer tools and technologies to address and enhance plant water use efficiency, water conservation, and reduce energy consumption for irrigation. The demonstration projects are supported by the scientifically‐based field research and evaluation projects conducted at the University of Nebraska‐Lincoln, South Central Agricultural Laboratory located near Clay Center, Nebraska. The Network was formed with only 15 farmers as collaborators in only one of the 23 NRDs in 2005. As of late 2009, the number of active collaborators has increased to over 300 in 12 NRDs and 35 of 93 counties. The Network is impacting both water and energy conservation due to farmers adopting information and newer technologies for irrigation management. The NAWMDN is helping participants to improve irrigation management and efficiency by monitoring plant growth stages and development, soil moisture, and crop evapotranspiration. As a result, they are reducing irrigation water application amounts and associated energy savings is leading to greater profitability to participating farmers. This article describes the goals and objectives of the Network, technical and educational components, operational functions, and procedures used in the NAWMDN. The quantitative impacts in terms of water and energy conservation are reported

Molecular Characterization, Tissue Distribution, Subcellular Localization and Actin-Sequestering Function of a Thymosin Protein from Silkworm

We identified a novel gene encoding a Bombyx mori thymosin (BmTHY) protein from a cDNA library of silkworm pupae, which has an open reading frame (ORF) of 399 bp encoding 132 amino acids. It was found by bioinformatics that BmTHY gene consisted of three exons and two introns and BmTHY was highly homologous to thymosin betas (Tβ). BmTHY has a conserved motif LKHTET with only one amino acid difference from LKKTET, which is involved in Tβ binding to actin. A His-tagged BmTHY fusion protein (rBmTHY) with a molecular weight of approximately 18.4 kDa was expressed and purified to homogeneity. The purified fusion protein was used to produce anti-rBmTHY polyclonal antibodies in a New Zealand rabbit. Subcellular localization revealed that BmTHY can be found in both Bm5 cell (a silkworm ovary cell line) nucleus and cytoplasm but is primarily located in the nucleus. Western blotting and real-time RT-PCR showed that during silkworm developmental stages, BmTHY expression levels are highest in moth, followed by instar larvae, and are lowest in pupa and egg. BmTHY mRNA was universally distributed in most of fifth-instar larvae tissues (except testis). However, BmTHY was expressed in the head, ovary and epidermis during the larvae stage. BmTHY formed complexes with actin monomer, inhibited actin polymerization and cross-linked to actin. All the results indicated BmTHY might be an actin-sequestering protein and participate in silkworm development

Consensus criteria for sensitive detection of minimal neuroblastoma cells in bone marrow, blood and stem cell preparations by immunocytology and QRT-PCR: recommendations by the International Neuroblastoma Risk Group Task Force

Disseminating disease is a predictive and prognostic indicator of poor outcome in children with neuroblastoma. Its accurate and sensitive assessment can facilitate optimal treatment decisions. The International Neuroblastoma Risk Group (INRG) Task Force has defined standardised methods for the determination of minimal disease (MD) by immunocytology (IC) and quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) using disialoganglioside GD2 and tyrosine hydroxylase mRNA respectively. The INRG standard operating procedures (SOPs) define methods for collecting, processing and evaluating bone marrow (BM), peripheral blood (PB) and peripheral blood stem cell harvest by IC and QRT-PCR. Sampling PB and BM is recommended at diagnosis, before and after myeloablative therapy and at the end of treatment. Peripheral blood stem cell products should be analysed at the time of harvest. Performing MD detection according to INRG SOPs will enable laboratories throughout the world to compare their results and thus facilitate quality-controlled multi-centre prospective trials to assess the clinical significance of MD and minimal residual disease in heterogeneous patient groups