Effects of isospin and momentum dependent interactions on thermal properties of asymmetric nuclear matter

Thermal properties of asymmetric nuclear matter are studied within a self-consistent thermal model using an isospin and momentum dependent interaction (MDI) constrained by the isospin diffusion data in heavy-ion collisions, a momentum-independent interaction (MID), and an isoscalar momentum-dependent interaction (eMDYI). In particular, we study the temperature dependence of the isospin-dependent bulk and single-particle properties, the mechanical and chemical instabilities, and liquid-gas phase transition in hot asymmetric nuclear matter. Our results indicate that the temperature dependence of the equation of state and the symmetry energy are not so sensitive to the momentum dependence of the interaction. The symmetry energy at fixed density is found to generally decrease with temperature and for the MDI interaction the decrement is essentially due to the potential part. It is further shown that only the low momentum part of the single-particle potential and the nucleon effective mass increases significantly with temperature for the momentum-dependent interactions. For the MDI interaction, the low momentum part of the symmetry potential is significantly reduced with increasing temperature. For the mechanical and chemical instabilities as well as the liquid-gas phase transition in hot asymmetric nuclear matter, our results indicate that the boundary of these instabilities and the phase-coexistence region generally shrink with increasing temperature and is sensitive to the density dependence of the symmetry energy and the isospin and momentum dependence of the nuclear interaction, especially at higher temperatures.Comment: 21 pages, 29 figure

Human Gingiva-Derived Mesenchymal Stromal Cells Attenuate Contact Hypersensitivity via Prostaglandin E2- Dependent Mechanisms

The immunomodulatory and anti-inflammatory functions of mesenchymal stromal cells (MSCs) have been demonstrated in several autoimmune/inflammatory disease models, but their contribution to the mitigation of contact hypersensitivity (CHS) remains unclear. Here, we report a new immunological approach using human gingiva-derived MSCs (GMSCs) to desensitize and suppress CHS and the underlying mechanisms. Our results showed that systemic infusion of GMSCs before the sensitization and challenge phase dramatically suppress CHS, manifested as a decreased infiltration of dendritic cells (DCs), CD8 + T cells, T H-17 and mast cells (MCs), a suppression of a variety of inflammatory cytokines, and a reciprocal increased infiltration of regulatory T cells and expression of IL-10 at the regional lymph nodes and the allergic contact areas. The GMSC-mediated immunosuppressive effects and mitigation of CHS were significantly abrogated on pretreatment with indomethacin, an inhibitor of cyclooxygenases. Under coculture condition of direct cell-cell contact or via transwell system, GMSCs were capable of direct suppression of differentiation of DCs and phorbol 12-myristate 13-acetate-stimulated activation of MCs, whereas the inhibitory effects were attenuated by indomethacin. Mechanistically, GMSC-induced blockage of de novo synthesis of proinflammatory cytokines by MCs is mediated partly by the tumor necrosis factor-alpha/prostaglandin E 2 (PGE 2) feedback axis. These results demonstrate that GMSCs are capable of desensitizing allergic contact dermatitis via PGE 2-dependent mechanisms. © AlphaMed Press

Human Gingiva-Derived Mesenchymal Stem Cells Elicit Polarization of M2 Macrophages and Enhance Cutaneous Wound Healing

Increasing evidence has supported the important role of mesenchymal stem cells (MSCs) in wound healing, however, the underlying mechanism remains unclear. Recently, we have isolated a unique population of MSCs from human gingiva (GMSCs) with similar stem cell-like properties, immunosuppressive, and anti-inflammatory functions as human bone marrow-derived MSCs (BMSCs). We describe here the interplay between GMSCs and macrophages and the potential relevance in skin wound healing. When cocultured with GMSCs, macrophages acquired an anti-inflammatory M2 phenotype characterized by an increased expression of mannose receptor (MR; CD206) and secretory cytokines interleukin (IL)-10 and IL-6, a suppressed production of tumor necrosis factor (TNF)-α, and decreased ability to induce Th-17 cell expansion. In vivo, we demonstrated that systemically infused GMSCs could home to the wound site in a tight spatial interaction with host macrophages, promoted them toward M2 polarization, and significantly enhanced wound repair. Mechanistically, GMSC treatment mitigated local inflammation mediated by a suppressed infiltration of inflammatory cells and production of IL-6 and TNF-α, and an increased expression of IL-10. The GMSC-induced suppression of TNF-α secretion by macrophages appears to correlate with impaired activation of NFκB p50. These findings provide first evidence that GMSCs are capable to elicit M2 polarization of macrophages, which might contribute to a marked acceleration of wound healing. © AlphaMed Press

Gravity-driven Magnetic Field at ∼1000 au Scales in High-mass Star Formation

A full understanding of high-mass star formation requires the study of one of the most elusive components of the energy balance in the interstellar medium: magnetic fields. We report Atacama Large Millimeter/submillimeter Array (ALMA) 1.2 mm, high-resolution (700 au) dust polarization and molecular line observations of the rotating hot molecular core embedded in the high-mass star-forming region IRAS 18089-1732. The dust continuum emission and magnetic field morphology present spiral-like features resembling a whirlpool. The velocity field traced by the H13CO+ (J = 3-2) transition line reveals a complex structure with spiral filaments that are likely infalling and rotating, dragging the field with them. We have modeled the magnetic field and find that the best model corresponds to a weakly magnetized core with a mass-to-magnetic-flux ratio (λ) of 8.38. The modeled magnetic field is dominated by a poloidal component, but with an important contribution from the toroidal component that has a magnitude of 30% of the poloidal component. Using the Davis-Chandrasekhar-Fermi method, we estimate a magnetic field strength of 3.5 mG. At the spatial scales accessible to ALMA, an analysis of the energy balance of the system indicates that gravity overwhelms turbulence, rotation, and the magnetic field. We show that high-mass star formation can occur in weakly magnetized environments, with gravity taking the dominant role.Fil: Sanhueza, Patricio. National Astronomical Observatory Of Japan; JapónFil: Girart, Josep Miquel. Instituto de Estudios Espaciales de Cataluña; EspañaFil: Padovani, Marco. Osservatorio Astrofisico Di Arcetri; ItaliaFil: Galli, Daniele. Osservatorio Astrofisico Di Arcetri; ItaliaFil: Hull, Charles L. H.. Atacama Large Millimeter-submillimeter Array; ChileFil: Zhang, Qizhou. Harvard-Smithsonian Center for Astrophysics; Estados UnidosFil: Cortes, Paulo. National Radio Astronomy Observatory; Estados UnidosFil: Stephens, Ian. Worcester State University; Estados UnidosFil: Fernandez Lopez, Manuel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Jackson, James M.. NASA Ames Research Center; Estados UnidosFil: Frau, Pau. Csic. Instituto de Ciencias del Espacio; EspañaFil: Kock, Patrick M.. Academia Sinica; ChinaFil: Wu, Benjamin. National Astronomical Observatory Of Japan; JapónFil: Zapata, Luis A.. Instituto de Radioastronomía y Astrofísica; MéxicoFil: Olguin, Fernando. National Tsing Hua University; ChinaFil: Lu, Xing. National Astronomical Observatory Of Japan; JapónFil: Silva, Andrea. National Astronomical Observatory Of Japan; JapónFil: Tang, Ya Wen. Academia Sinica; ChinaFil: Sakai, Takeshi. The University Of Electro-communications; JapónFil: Guzmán, Andrés E.. National Astronomical Observatory Of Japan; JapónFil: Tatematsu, Ken'Ichi. National Astronomical Observatory Of Japan; JapónFil: Nakamura, Fumitaka. National Astronomical Observatory Of Japan; JapónFil: Chen, Huei Ru Vivien. National Tsing Hua University; Chin

Primary adenomyoepithelioma of tonsil

We present a case of adenomyoepithlioma (AME) arising from the tonsil. AME is an uncommon tumor that typically arises in breast, but rarely found in salivary glands, lung, and skin. Its biological features have not been thoroughly characterized. Here we describe a primary AME originating from the tonsil. The pathologic changes were characterized by hypercellularity, the dominance of both epithelial and myoepithelial cells. Malignancy was evidenced by the presence of a high mitotic rate and invasive growth. The epithelial cells express high levels of cytokeratin and epithelial membrane antigen (EMA). The myoepithelial cells show positive staining for calponin, p63, vimentin, and S-100. A thorough review of the literature indicates that this is likely the first reported case of AME from the tonsil. Following descriptions of the diagnosis, treatment, and prognosis of this specific case, pathologic and clinical characteristics of AME from other tissues are also compiled and discussed

BCAR1 Protein Plays Important Roles in Carcinogenesis and Predicts Poor Prognosis in Non-Small-Cell Lung Cancer

Objective: Our previous study suggested the potential clinical implications of BCAR1 in non-small-cell lung cancer (NSCLC) (Mol Diagn Ther. 2011. 15(1): 31–40). Herein, we aim to evaluate the predictive power of BCAR1 as a marker for poor prognosis in NSCLC cases, verify the carcinogenic roles of BCAR1 in the A549 lung adenocarcinoma cell line, and testify to the BCAR1/phospho-p38 axis. Methods: Between January 2006 and June 2010, there were a total of 182 patients with NSCLC (151 cases with available follow up data, and 31 cases lost to follow-up due to the invalid contact information). We inspected BCAR1, phospho-BCAR1(Tyr410), phospho-p38(Thr180/Tyr182) and p38 expression in NSCLC tissues and matched adjacent normal tissues by immunoblotting and IHC. After BCAR1-RNA interference in A549 cells, we inspected the protein expression (BCAR1, phospho-BCAR1, phospho-p38 and p38) and performed cell biology experiments (cell growth, migration and cycle). Results: BCAR1 was overexpressed in NSCLC tissues (177/182) and cell lines (A549 and Calu-3). However, it was not detected in the normal adjacent tissue in 161 of the 182 cases. Higher BCAR1 levels were strongly associated with more poorly differentiated NSCLC and predicted poorer prognosis. BCAR1 knockdown caused cell growth arrest, cell migration inhibition and cell cycle arrest of A549 cells. Overexpression of BCAR1 was associated with activation of p38 in NSCLC cases, and BCAR1 knockdown caused reduction of phospho-p38 levels in A549 cells

Stochastic Gravity: Theory and Applications

Whereas semiclassical gravity is based on the semiclassical Einstein equation with sources given by the expectation value of the stress-energy tensor of quantum fields, stochastic semiclassical gravity is based on the Einstein-Langevin equation, which has in addition sources due to the noise kernel. In the first part, we describe the fundamentals of this new theory via two approaches: the axiomatic and the functional. In the second part, we describe three applications of stochastic gravity theory. First, we consider metric perturbations in a Minkowski spacetime, compute the two-point correlation functions of these perturbations and prove that Minkowski spacetime is a stable solution of semiclassical gravity. Second, we discuss structure formation from the stochastic gravity viewpoint. Third, we discuss the backreaction of Hawking radiation in the gravitational background of a black hole and describe the metric fluctuations near the event horizon of an evaporating black holeComment: 100 pages, no figures; an update of the 2003 review in Living Reviews in Relativity gr-qc/0307032 ; it includes new sections on the Validity of Semiclassical Gravity, the Stability of Minkowski Spacetime, and the Metric Fluctuations of an Evaporating Black Hol

De novo design of potent and resilient hACE2 decoys to neutralize SARS-CoV-2

We developed a de novo protein design strategy to swiftly engineer decoys for neutralizing pathogens that exploit extracellular host proteins to infect the cell. Our pipeline allowed the design, validation, and optimization of de novo hACE2 decoys to neutralize SARS-CoV-2. The best decoy, CTC-445.2, binds with low nanomolar affinity and high specificity to the RBD of the spike protein. Cryo-EM shows that the design is accurate and can simultaneously bind to all three RBDs of a single spike protein. Because the decoy replicates the spike protein target interface in hACE2, it is intrinsically resilient to viral mutational escape. A bivalent decoy, CTC-445.2d, shows ~10-fold improvement in binding. CTC-445.2d potently neutralizes SARS-CoV-2 infection of cells in vitro and a single intranasal prophylactic dose of decoy protected Syrian hamsters from a subsequent lethal SARS-CoV-2 challenge

Self-Assembly Fabrication of Hollow Mesoporous Silica@Co–Al Layered Double Hydroxide@Graphene and Application in Toxic Effluents Elimination

Here, we propose a self-assembly process to prepare hierarchical HM-SiO2@Co–Al LDH@graphene, with the purpose of combining their outstanding performance. Hollow mesoporous silica was first synthesized as the core, using a novel sonochemical method, followed by a controlled shell coating process and chemical reduction. As a result of the electrostatic potential difference among HM-SiO2, Co–Al LDH, and graphene oxide, the HM-SiO2 spheres were coated by Co–Al LDH and graphene. Subsequently, the HM-SiO2@Co–Al LDH@graphene spheres were introduced into an epoxy resin (EP) matrix for investigation of their toxic effluents capture and elimination effectiveness during combustion. The amount of toxic CO and volatile organic compounds from the epoxy resin decomposition significantly suppressed after incorporating the HM-SiO2@Co–Al LDH@graphene hybrids, implying a reduced toxicity