The Effects of Prehydration on Cement Performance

This study investigated the effects of cement prehydration on cement’s engineering properties. Anhydrous cement was exposed over a saturated KCl solution to maintain 85% RH, for 7 and 28 days. Mortar and cement pastes were tested for strength, workability and setting time, with sample analysis by XRD and DTA. Results showed a decreased reactivity of the prehydrated cements resulting in reduced strength and increased setting times. We propose that this may be due to an upset of the sulphate balance in the cement upon prehydration

Sing Me an Old Song

Sing Me An Old Song expresses the feelings I experienced growing up in a hymn singing church and then changing worship style in college

The Role of the School Administrator in Implementing Communities of Practice to Build Organizational Capacity

The field of education is constantly changing and requires a significant shift in teacher practice. Despite the demands for ongoing professional learning and collaboration, teachers remain resistant to the transition from an autonomous position. Professional learning communities, a particular type of communities of practice, are a common practice for schools to implement with a goal of improving student performance. Despite attempts, this concept proves to be very difficult to implement. The purpose of this study was to examine the nature of existing professional learning communities within the school setting. In addition, this study sought to understand the role of school administrators in implementing and sustaining communities of practice. Factors that succeed and hinder communities of practice are confirmed and extended, in addition to identifying how this concept is related to school improvement and organizational capacity

Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA.

Escherichia coli RecA is the defining member of a ubiquitous class of DNA strand-exchange proteins that are essential for homologous recombination, a pathway that maintains genomic integrity by repairing broken DNA. To function, filaments of RecA must nucleate and grow on single-stranded DNA (ssDNA) in direct competition with ssDNA-binding protein (SSB), which rapidly binds and continuously sequesters ssDNA, kinetically blocking RecA assembly. This dynamic self-assembly on a DNA lattice, in competition with another protein, is unique for the RecA family compared to other filament-forming proteins such as actin and tubulin. The complexity of this process has hindered our understanding of RecA filament assembly because ensemble measurements cannot reliably distinguish between the nucleation and growth phases, despite extensive and diverse attempts. Previous single-molecule assays have measured the nucleation and growth of RecA--and its eukaryotic homologue RAD51--on naked double-stranded DNA and ssDNA; however, the template for RecA self-assembly in vivo is SSB-coated ssDNA. Using single-molecule microscopy, here we directly visualize RecA filament assembly on single molecules of SSB-coated ssDNA, simultaneously measuring nucleation and growth. We establish that a dimer of RecA is required for nucleation, followed by growth of the filament through monomer addition, consistent with the finding that nucleation, but not growth, is modulated by nucleotide and magnesium ion cofactors. Filament growth is bidirectional, albeit faster in the 5'→3' direction. Both nucleation and growth are repressed at physiological conditions, highlighting the essential role of recombination mediators in potentiating assembly in vivo. We define a two-step kinetic mechanism in which RecA nucleates on transiently exposed ssDNA during SSB sliding and/or partial dissociation (DNA unwrapping) and then the RecA filament grows. We further demonstrate that the recombination mediator protein pair, RecOR (RecO and RecR), accelerates both RecA nucleation and filament growth, and that the introduction of RecF further stimulates RecA nucleation

Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3

double Holliday junction (dHJ) is a central intermediate of homologous recombination that can be processed to yield crossover or non-crossover recombination products. To preserve genomic integrity, cells possess mechanisms to avoid crossing over. We show that Saccharomyces cerevisiae Sgs1 and Top3 proteins are sufficient to migrate and disentangle a dHJ to produce exclusively non-crossover recombination products, in a reaction termed "dissolution." We show that Rmi1 stimulates dHJ dissolution at low Sgs1-Top3 protein concentrations, although it has no effect on the initial rate of Holliday junction (HJ) migration. Rmi1 serves to stimulate DNA decatenation, removing the last linkages between the repaired and template DNA molecules. Dissolution of a dHJ is a highly efficient and concerted alternative to nucleolytic resolution that prevents crossing over of chromosomes during recombinational DNA repair in mitotic cells and thereby contributes to genomic integrity

The impact of the AuScope VLBI observations and the regional AUSTRAL sessions on the TRF

The AuScope VLBI array was built with the purpose to improve the terrestrial (TRF) and celestial reference frames in the southern hemisphere. Since 2010 the three 12-m antennas in Hobart (Tasmania), Katherine (Northern Territory) and Yarragadee (Western Australia) heavily contribute to the global VLBI observations coordinated by the International VLBI Service for Geodesy and Astrometry. In 2011, the AUSTRAL VLBI program was started, with more than 40 sessions being observed so far. In the AUSTRALs, the three AuScope antennas observe together with the new 15-m dish in Hartebeesthoek (South Africa) and the 12-m antenna in Warkworth (New Zealand). Recently, the planned observations have been expanded again, with 50 additional sessions scheduled until mid-2015, along with 3 continuous campaigns covering 15 days each. All AUSTRALs are recorded with an increased data rate of 1 Gbps, allowing to compensate for the reduced sensitivity of the generally smaller dish size. We evaluate the positive impact of the AuScope VLBI program on the global TRF. This is due to the increased number of observations and the improved homogeneity of the global VLBI network. All data collected within this intense observing program is analysed and geodetic results are presented. This includes time series of baseline lengths and station coordinates of the contributing stations. We compare the results obtained within the regional AUSTRAL sessions with the ones of the classical global VLBI networks and identify superiorities and shortcomings of both. The high number of sessions gives high accuracies and good repeatabilities of the determined parameters. Additionally, remaining variations of baseline lengths can be identified and are compared against by default un-modelled station motions due to hydrology and atmosphere loading. Finally, we give an outlook on future plans for the AuScope antennas and the AUSTRAL observing program: on future operations, expected improvements through hardware upgrades as well as research on the use of sibling telescopes available at two sites within the AUSTRAL array (Hobart and Hartebeesthoek)

Mantle melting as a function of water content beneath back-arc basins

Subduction zone magmas are characterized by high concentrations of H_(2)O, presumably derived from the subducted plate and ultimately responsible for melting at this tectonic setting. Previous studies of the role of water during mantle melting beneath back-arc basins found positive correlations between the H_(2)O concentration of the mantle (H_(2)O_o ) and the extent of melting (F), in contrast to the negative correlations observed at mid-ocean ridges. Here we examine data compiled from six back-arc basins and three mid-ocean ridge regions. We use TiO_2 as a proxy for F, then use F to calculate H_(2)O_o from measured H_(2)O concentrations of submarine basalts. Back-arc basins record up to 0.5 wt % H_(2)O or more in their mantle sources and define positive, approximately linear correlations between H_(2)O_o and F that vary regionally in slope and intercept. Ridge-like mantle potential temperatures at back-arc basins, constrained from Na-Fe systematics (1350°–1500°C), correlate with variations in axial depth and wet melt productivity (∼30–80% F/wt % H_(2)O_o ). Water concentrations in back-arc mantle sources increase toward the trench, and back-arc spreading segments with the highest mean H_(2)O_o are at anomalously shallow water depths, consistent with increases in crustal thickness and total melt production resulting from high H_(2)O. These results contrast with those from ridges, which record low H_(2)O_o (<0.05 wt %) and broadly negative correlations between H_(2)O_o and F that result from purely passive melting and efficient melt focusing, where water and melt distribution are governed by the solid flow field. Back-arc basin spreading combines ridge-like adiabatic melting with nonadiabatic mantle melting paths that may be independent of the solid flow field and derive from the H_(2)O supply from the subducting plate. These factors combine significant quantitative and qualitative differences in the integrated influence of water on melting phenomena in back-arc basin and mid-ocean ridge settings

Aging and central vision loss: Relationship between the cortical macro-structure and micro-structure

Aging and central vision loss are associated with cortical atrophies, but little is known about the relationship between cortical thinning and the underlying cellular structure. We compared the macro- and micro-structure of the cortical gray and superficial white matter of 38 patients with juvenile (JMD) or age-related (AMD) macular degeneration and 38 healthy humans (19-84 years) by multimodal MRI including diffusion-tensor imaging (DTI). A factor analysis showed that cortical thickness, tissue-dependent measures, and DTI-based measures were sensitive to distinct components of brain structure. Age-related cortical thinning and increased diffusion were observed across most of the cortex, but increased T1-weighted intensities (frontal), reduced T2-weighted intensities (occipital), and reduced anisotropy (medial) were limited to confined cortical regions. Vision loss was associated with cortical thinning and enhanced diffusion in the gray matter (less in the white matter) of the occipital central visual field representation. Moreover, AMD (but not JMD) patients showed enhanced diffusion in lateral occipito-temporal cortex and cortical thinning in the posterior cingulum. These findings demonstrate that changes in brain structure are best quantified by multimodal imaging. They further suggest that age-related brain atrophies (cortical thinning) reflect diverse micro-structural etiologies. Moreover, juvenile and age-related macular degeneration are associated with distinct patterns of micro-structural alterations