487 research outputs found

    Tau energy losses at ultra-high energy: continuous versus stochastic treatment

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    We study the energy losses of the tau lepton in matter through electromagnetic processes at ultra-high energy (UHE). We use both a stochastic and a continuous framework to treat these interactions and compare the flux of tau leptons propagated after some amount of matter. We discuss the accuracy of the approximation of continuous energy losses by studying the propagation in standard rock of taus with both mono-energetic and power law injection spectra.Comment: 7 pages, 8 figure

    Investigation of microstructure defects in EUROFER97 under Heâș/FeÂłâș dual ion beam irradiation

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    Fusion like conditions for reduced activation ferritic/martensitic steels in the first wall are simulated with single Fe3+ and He+/Fe3+ dual ion beam irradiation of EUROFER97 at the Jannus laboratory, CEA Saclay, introducing a damage of 16 dpa and a helium content up to 260 appm. The samples are irradiated at temperatures of 330 °C, 400 °C and 500 °C. The quantitative determination of size distribution and density of dislocation loops is obtained using weak-beam dark-field imaging mode. Burgers vectors of a02〈111âŒȘ are observed for the majority of dislocation loops at irradiation temperatures of 330 °C and 400 °C. At 500 °C no dislocation loops are found. The impact of single and dual ion beam irradiation on mechanical properties is determined by means of nanoindentation. An increase in nano-hardness of up to 35% due to irradiation was measured at samples irradiated at 400 °C. A kinetic rate model is applied for the description of nucleation and evolution of helium bubbles and compared with the experimental results. Evaluating the rate model with help of TEM-results for size and density of bubbles indicates the nucleation scheme as the main source for quantitative disagreement between the model and irradiation. Keywords: Radiation effects, Ion irradiation, Cluster dynamics, Fusion, Helium bubbles, RAFM steel

    Demographic histories shape population genomics of the common coral grouper (Plectropomus leopardus)

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    Many coral reef fishes display remarkable genetic and phenotypic variation across their geographic ranges. Understanding how historical and contemporary processes have shaped these patterns remains a focal question in evolutionary biology since they reveal how diversity is generated and how it may respond to future environmental change. Here, we compare the population genomics and demographic histories of a commercially and ecologically important coral reef fish, the common coral grouper (Plectropomus leopardus [Lacepede 1802]), across two adjoining regions (the Great Barrier Reef; GBR, and the Coral Sea, Australia) spanning approximately 14 degrees of latitude and 9 degrees of longitude. We analysed 4548 single nucleotide polymorphism (SNP) markers across 11 sites and show that genetic connectivity between regions is low, despite their relative proximity (similar to 100km) and an absence of any obvious geographic barrier. Inferred demographic histories using 10,479 markers suggest that the Coral Sea population was founded by a small number of GBR individuals and that divergence occurred similar to 190 kya under a model of isolation with asymmetric migration. We detected population expansions in both regions, but estimates of contemporary effective population sizes were approximately 50% smaller in Coral Sea sites, which also had lower genetic diversity. Our results suggest that P. leopardus in the Coral Sea have experienced a long period of isolation that precedes the recent glacial period (similar to 10-120 kya) and may be vulnerable to localized disturbances due to their relative reliance on local larval replenishment. While it is difficult to determine the underlying events that led to the divergence of the Coral Sea and GBR lineages, we show that even geographically proximate populations of a widely dispersed coral reef fish can have vastly different evolutionary histories

    Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage

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    <p>Abstract</p> <p>Background</p> <p><it>Vanilla planifolia </it>is an important Orchid commercially cultivated for the production of natural vanilla flavour. Vanilla plants are conventionally propagated by stem cuttings and thus causing injury to the mother plants. Regeneration and <it>in vitro </it>mass multiplication are proposed as an alternative to minimize damage to mother plants. Because mass production of <it>V. planifolia </it>through indirect shoot differentiation from callus culture is rare and may be a successful use of in <it>vitro </it>techniques for producing somaclonal variants, we have established a novel protocol for the regeneration of vanilla plants and investigated the initial biochemical and molecular mechanisms that trigger shoot organogenesis from embryogenic/organogenic callus.</p> <p>Results</p> <p>For embryogenic callus induction, seeds obtained from 7-month-old green pods of <it>V. planifolia </it>were inoculated on MS basal medium (BM) containing TDZ (0.5 mg l<sup>-1</sup>). Germination of unorganized mass callus such as protocorm -like structure (PLS) arising from each seed has been observed. The primary embryogenic calli have been formed after transferring on BM containing IAA (0.5 mg l<sup>-1</sup>) and TDZ (0.5 mg l<sup>-1</sup>). These calli were maintained by subculturing on BM containing IAA (0.5 mg l<sup>-1</sup>) and TDZ (0.3 mg l<sup>-1</sup>) during 6 months and formed embryogenic/organogenic calli. Histological analysis showed that shoot organogenesis was induced between 15 and 20 days after embryogenic/organogenic calli were transferred onto MS basal medium with NAA (0.5 mg l<sup>-1</sup>). By associating proteomics and metabolomics analyses, the biochemical and molecular markers responsible for shoot induction have been studied in 15-day-old calli at the stage where no differentiating part was visible on organogenic calli. Two-dimensional electrophoresis followed by matrix-assisted laser desorption ionization time-of-flight-tandem mass spectrometry (MALDI-TOF-TOF-MS) analysis revealed that 15 protein spots are significantly expressed (<it>P </it>< 0.05) at earlier stages of shoot differentiation. The majority of these proteins are involved in amino acid-protein metabolism and photosynthetic activity. In accordance with proteomic analysis, metabolic profiling using 1D and 2D NMR techniques showed the importance of numerous compounds related with sugar mobilization and nitrogen metabolism. NMR analysis techniques also allowed the identification of some secondary metabolites such as phenolic compounds whose accumulation was enhanced during shoot differentiation.</p> <p>Conclusion</p> <p>The subculture of embryogenic/organogenic calli onto shoot differentiation medium triggers the stimulation of cell metabolism principally at three levels namely (i) initiation of photosynthesis, glycolysis and phenolic compounds synthesis; (ii) amino acid - protein synthesis, and protein stabilization; (iii) sugar degradation. These biochemical mechanisms associated with the initiation of shoot formation during protocorm - like body (PLB) organogenesis could be coordinated by the removal of TDZ in callus maintenance medium. These results might contribute to elucidate the complex mechanism that leads to vanilla callus differentiation and subsequent shoot formation into PLB organogenesis. Moreover, our results highlight an early intermediate metabolic event in vanillin biosynthetic pathway with respect to secondary metabolism. Indeed, for the first time in vanilla tissue culture, phenolic compounds such as glucoside A and glucoside B were identified. The degradation of these compounds in specialized tissue (i.e. young green beans) probably contributes to the biosynthesis of glucovanillin, the parent compound of vanillin.</p

    Tritiated Steel Micro-Particles: Computational Dosimetry and Prediction of Radiation-Induced DNA Damage for In Vitro Cell Culture Exposures.

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    Biological effects of radioactive particles can be experimentally investigated in vitro as a function of particle concentration, specific activity and exposure time. However, a careful dosimetric analysis is needed to elucidate the role of radiation emitted by radioactive products in inducing cyto- and geno-toxicity: the quantification of radiation dose is essential to eventually inform dose-risk correlations. This is even more fundamental when radioactive particles are short-range emitters and when they have a chemical speciation that might further concur to the heterogeneity of energy deposition at the cellular and sub-cellular level. To this aim, we need to use computational models. In this work, we made use of a Monte Carlo radiation transport code to perform a computational dosimetric reconstruction for in vitro exposure of cells to tritiated steel particles of micrometric size. Particles of this kind have been identified as worth of attention in nuclear power industry and research: tritium easily permeates in steel elements of nuclear reactor machinery, and mechanical operations on these elements (e.g., sawing) during decommissioning of old facilities can result in particle dispersion, leading to human exposure via inhalation. Considering the software replica of a representative in vitro setup to study the effect of such particles, we therefore modelled the radiation field due to the presence of particles in proximity of cells. We developed a computational approach to reconstruct the dose range to individual cell nuclei in contact with a particle, as well as the fraction of "hit" cells and the average dose for the whole cell population, as a function of particle concentration in the culture medium. The dosimetric analysis also provided the basis to make predictions on tritium-induced DNA damage: we estimated the dose-dependent expected yield of DNA double strand breaks due to tritiated steel particle radiation, as an indicator of their expected biological effectiveness

    Radio emission of extensive air shower at CODALEMA: Polarization of the radio emission along the v*B vector

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    Cosmic rays extensive air showers (EAS) are associated with transient radio emission, which could provide an efficient new detection method of high energy cosmic rays, combining a calorimetric measurement with a high duty cycle. The CODALEMA experiment, installed at the Radio Observatory in Nancay, France, is investigating this phenomenon in the 10^17 eV region. One challenging point is the understanding of the radio emission mechanism. A first observation indicating a linear relation between the electric field produced and the cross product of the shower axis with the geomagnetic field direction has been presented (B. Revenu, this conference). We will present here other strong evidences for this linear relationship, and some hints on its physical origin.Comment: Contribution to the 31st International Cosmic Ray Conference, Lodz, Poland, July 2009. 4 pages, 8 figures. v2: Typo fixed, arxiv references adde

    Remote marine protected area reveals unusual social behaviour in Chaetodon trifascialis

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    Author Posting. © The Author(s), 2016. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Marine Biodiversity 48 (2018): 155-156, doi:10.1007/s12526-016-0531-0.The research expedition was funded by New England Aquarium, Woods Hole Oceanographic Institution and King Abdullah University of Science and Technology

    Design of an interaction region with head-on collisions for the ILC

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    An interaction region (IR) with head-on collisions is considered as an alternative to the baseline configuration of the International Linear Collider (ILC) which includes two IRs with finite crossing-angles (2 and 20 mrad). Although more challenging for the beam extraction, the head-on scheme is favoured by the experiments because it allows a more convenient detector configuration, particularly in the forward region. The optics of the head-on extraction is revisited by separating the e+ and e- beams horizontally, first by electrostatic separators operated at their LEP nominal field and then using a defocusing quadrupole of the final focus beam line. In this way the septum magnet is protected from the beamstrahlung power. Newly optimized final focus and extraction optics are presented, including a first look at post-collision diagnostics. The influence of parasitic collisions is shown to lead to a region of stable collision parameters. Disrupted beam and beamstrahlung photon losses are calculated along the extraction elements
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