Routine testing of fetal Rhesus D status in Rhesus D negative women using cell-free fetal DNA: An investigation into the preferences and information needs of women

Objective: The goal of this study is to investigate women's preferences and information needs for routine implementation of fetal Rhesus D (RhD) typing using cell-free fetal DNA. Methods: A questionnaire was developed following focus groups and interviews with both health professionals and RhD negative (RhD-) women offered fetal RhD genotyping within a research study and distributed to RhD- women attending routine antenatal appointments in four National Health Service hospitals. Current knowledge of blood types, anti-D administration, fetal RhD genotyping and future practices were explored. Results: A total of 19 respondents participated in interviews and focus groups, and 270 respondents completed the questionnaires. Questionnaire respondents overwhelmingly felt that the test should be offered to all RhD- women (92.1%), and 75.9% said that they would accept this test. Most were happy to have the test even if it involved extra blood tests (89.3%) or appointments (79%). The knowledge of blood groups was poor. Although 90.7% knew that the baby could have a different blood group from themselves, only 34% knew that blood groups are inherited from both parents. More than 40% were not aware that anti-D would not be required if their baby was RhD-. Conclusions: Women would welcome the introduction of routine fetal RhD genotyping. Information leaflets and training of midwives will be essential for implementation to ensure good understanding regarding testing. © 2013 John Wiley & Sons, Ltd. © 2013 John Wiley & Sons, Ltd

Caribbean Corals in Crisis: Record Thermal Stress, Bleaching, and Mortality in 2005

BACKGROUND The rising temperature of the world's oceans has become a major threat to coral reefs globally as the severity and frequency of mass coral bleaching and mortality events increase. In 2005, high ocean temperatures in the tropical Atlantic and Caribbean resulted in the most severe bleaching event ever recorded in the basin. METHODOLOGY/PRINCIPAL FINDINGS Satellite-based tools provided warnings for coral reef managers and scientists, guiding both the timing and location of researchers' field observations as anomalously warm conditions developed and spread across the greater Caribbean region from June to October 2005. Field surveys of bleaching and mortality exceeded prior efforts in detail and extent, and provided a new standard for documenting the effects of bleaching and for testing nowcast and forecast products. Collaborators from 22 countries undertook the most comprehensive documentation of basin-scale bleaching to date and found that over 80% of corals bleached and over 40% died at many sites. The most severe bleaching coincided with waters nearest a western Atlantic warm pool that was centered off the northern end of the Lesser Antilles. CONCLUSIONS/SIGNIFICANCE Thermal stress during the 2005 event exceeded any observed from the Caribbean in the prior 20 years, and regionally-averaged temperatures were the warmest in over 150 years. Comparison of satellite data against field surveys demonstrated a significant predictive relationship between accumulated heat stress (measured using NOAA Coral Reef Watch's Degree Heating Weeks) and bleaching intensity. This severe, widespread bleaching and mortality will undoubtedly have long-term consequences for reef ecosystems and suggests a troubled future for tropical marine ecosystems under a warming climate.This work was partially supported by salaries from the NOAA Coral Reef Conservation Program to the NOAA Coral Reef Conservation Program authors. NOAA provided funding to Caribbean ReefCheck investigators to undertake surveys of bleaching and mortality. Otherwise, no funding from outside authors' institutions was necessary for the undertaking of this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Proceedings IMWA 2010

Abstract A research-grade passive treatment system was constructed to receive 1000 L/minute of mine water from abandoned boreholes (pH 5.95, net alkalinity 29 mg/L CaCO₃, Fe 192 mg/L, Zn 11 mg/L, Cd 17 μg/L, Pb 60 μg/L and As 64 μg/L). The 2-ha system includes an oxidation pond followed by parallel treatment trains of aerobic wetlands, vertical flow bioreactors, re-aeration ponds, and horizontal-flow limestone beds and a final polishing wetland. Final effluent waters had pH >7 and contained < 1 mg/L total Fe and < 0.1 mg/L total Zn, with concentrations of Cd, Pb and As below detectable limits. Key Words hard rock mining, metal mining, acid mine drainage, natural treatment systems Introduction This paper describes the initial evaluation of an innovative, ecologically engineered passive system designed to treat abandoned ferruginous Pb-Zn mine waters at the Tar Creek Superfund Site, part of the historic Tri-State Mining District (TSMD) of Oklahoma, Kansas and Missouri, USA. Significant quantities of Pb and Zn were produced from the TSMD from the 1890s through the 1960s. By the early 1970s when mining ceased, two and nine million tons of Pb and Zn, respectively, had been produced During mining, large capacity dewatering operations pumped approximately 50,000 m³ d⁻¹ of water from the mines (Reed et al. 1955). Upon decline and cessation of mining, groundwater began to accumulate in the mine voids. By late 1979, metal-rich waters began to discharge via artesian pressure into Tar Creek and its tributaries. The first documented discharges of mine drainage were at a location near southeast Commerce, OK (Oklahoma Water Resources Board 1983) and were subsequently identified for passive treatment implementation Methods For this study, periodic water quality and quantity data collection efforts for the subject discharges began in 1998, with regular monthly sampling beginning in 2004 and continuing to the present. The targeted discharges have circum-neutral pH (5.96 ± 0.06), total alkalinity of 405 ± 13 mg/L as CaCO₃ and combined flow rates of up to 1000 L/minute. Metals and sulfate concentrations are elevated above expected levels and degrade the receiving waters ). Design and construction details for the passive treatment system are summarized in Sydney, NS IMWA 2010 "Mine Water and Innovative Thinking" Wolkersdorfer & Freund Results and Discussion In the year of operation, the passive treatment system performed as designed from a water quality perspective ( Other metals of specific interest in these waters were Cd, Pb, and As. All three were removed to below detection limits (0.64, 19.5 and 22 µg/L, respectively) before the outflow of the second process units, presumably through sorptive processes. Although the vertical-flow bioreactors were designed to remove Cd and Pb as well as Zn, Cd and Pb rarely remained in measureable concentrations at this stage of the treatment system. The other trace metal found in significant concentrations in these waters was Ni. A small percentage (<10%) of Ni was removed through co-precipitation and sorption in Cell 1. However, the majority of Ni (≈ 95%) was removed via re- IMWA 2010 Sydney, NS "Mine Water and Innovative Thinking&quot

Molecular systematics of flyingfishes (Teleostei: Exocoetidae): evolution in the epipelagic zone

The flyingfish family Exocoetidae is a diverse group of marine fishes that are widespread and abundant in tropical and subtropical seas. Flyingfishes are epipelagic specialists that are easily distinguished by their enlarged fins, which are used for gliding leaps over the surface of the water. Although phylogenetic hypotheses have been proposed for flyingfish genera based on morphology, no comprehensive molecular studies have been performed. In the present study, we describe a species-level molecular phylogeny for the family Exocoetidae, based on data from the mitochondrial cytochrome b gene (1137 bp) and the nuclear RAG2 gene (882 bp). We find strong support for previous morphology-based phylogenetic hypotheses, as well as the monophyly of most currently accepted flyingfish genera. However, the most diverse genus Cheilopogon is not monophyletic. Using our novel flyingfish topology, we examine previously proposed hypotheses for the origin and evolution of gliding. The results support the progressive transition from two-wing to four-wing gliding. We also use phylogenetic approaches to test the macroecological effects of two life history characters (e.g. egg buoyancy and habitat) on species range size in flyingfishes. © 2010 The Linnean Society of London, Biological Journal of the Linnean Society , 2011, 102, 161–174.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/79173/1/BIJ_1550_sm_Appendix_S1.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/79173/2/j.1095-8312.2010.01550.x.pd

The Conservation Status of Marine Bony Shorefishes of the Greater Caribbean

The greater Caribbean biogeographic region covered in this report (representing 38 countries and territories) encompasses an outstanding marine bony shorefish richness of approximately 1,360 species, with many (53%) being endemic. This report provides an overview of the conservation status of greater Caribbean shorefishes, with detailed information available through the IUCN Red List, and gives recommendations

A small-molecule PI3Kα activator for cardioprotection and neuroregeneration

Harnessing the potential beneficial effects of kinase signalling through the generation of direct kinase activators remains an underexplored area of drug development1,2,3,4,5. This also applies to the PI3K signalling pathway, which has been extensively targeted by inhibitors for conditions with PI3K overactivation, such as cancer and immune dysregulation. Here we report the discovery of UCL-TRO-1938 (referred to as 1938 hereon), a small-molecule activator of the PI3Kα isoform, a crucial effector of growth factor signalling. 1938 allosterically activates PI3Kα through a distinct mechanism by enhancing multiple steps of the PI3Kα catalytic cycle and causes both local and global conformational changes in the PI3Kα structure. This compound is selective for PI3Kα over other PI3K isoforms and multiple protein and lipid kinases. It transiently activates PI3K signalling in all rodent and human cells tested, resulting in cellular responses such as proliferation and neurite outgrowth. In rodent models, acute treatment with 1938 provides cardioprotection from ischaemia–reperfusion injury and, after local administration, enhances nerve regeneration following nerve crush. This study identifies a chemical tool to directly probe the PI3Kα signalling pathway and a new approach to modulate PI3K activity, widening the therapeutic potential of targeting these enzymes through short-term activation for tissue protection and regeneration. Our findings illustrate the potential of activating kinases for therapeutic benefit, a currently largely untapped area of drug development