A new model for the structure of the DACs and SACs regions in the Oe and Be stellar atmospheres

In this paper we present a new mathematical model for the density regions where a specific spectral line and its SACs/DACs are created in the Oe and Be stellar atmospheres. In the calculations of final spectral line function we consider that the main reasons of the line broadening are the rotation of the density regions creating the spectral line and its DACs/SACs, as well as the random motions of the ions. This line function is able to reproduce the spectral feature and it enables us to calculate some important physical parameters, such as the rotational, the radial and the random velocities, the Full Width at Half Maximum, the Gaussian deviation, the optical depth, the column density and the absorbed or emitted energy. Additionally, we can calculate the percentage of the contribution of the rotational velocity and the ions' random motions of the DACs/SACs regions to the line broadening. Finally, we present two tests and three short applications of the proposed model.Comment: 9 pages, 5 figures, accepted for publication in PAS

The basic chemistry of exercise-induced DNA oxidation:oxidative damage, redox signalling and their interplay

Acute exercise increases reactive oxygen and nitrogen species generation. This phenomenon is associated with two major outcomes: (1) redox signalling and (2) macromolecule damage. Mechanistic knowledge of how exercise-induced redox signalling and macromolecule damage are interlinked is limited. This review focuses on the interplay between exercise-induced redox signalling and DNA damage, using hydroxyl radical (·OH) and hydrogen peroxide (H2O2) as exemplars. It is postulated that the biological fate of H2O2 links the two processes and thus represents a bifurcation point between redox signalling and damage. Indeed, H2O2 can participate in two electron signalling reactions but its diffusion and chemical properties permit DNA oxidation following reaction with transition metals and ·OH generation. It is also considered that the sensing of DNA oxidation by repair proteins constitutes a non-canonical redox signalling mechanism. Further layers of interaction are provided by the redox regulation of DNA repair proteins and their capacity to modulate intracellular H2O2 levels. Overall, exercise-induced redox signalling and DNA damage may be interlinked to a greater extent than was previously thought but this requires further investigation

Association between physical activity patterns and anthropometric characteristics of adults : an issue of public health?

The aim of this study was to evaluate the association between objectively measured daily physical activity (PA) and body fat mass (BF) and body mass index (BMI). A further aim was to analyse the variance of PA between quartiles of BF and BMI. Material and methods: A cross-sectional, observational study of 126 university students (53 males aged 20.46 ± 2.04 years and 73 female aged 19.69 ± 1.32 years) was conducted. Results: The female participants and PA characteristics explain 57.10% of BF variance and the model was statistically significant (F(6, 875) = 196.38; p = 0.001). BMI was also included in the model. Standard binary logistic regression was used to test the hypothesis that female sex and PA characteristics can influence overweight. The full model containing all variables was statistically significant (G2(6) = 58.598, p-value = 0.001). Analysis of variance between BF quartiles revealed statistically significant differences in male participants in light PA (p = 0.001; ES = 0.09), moderate PA (p = 0.001; ES = 0.042) and vigorous PA (p = 0.001; ES = 0.130). Conclusions: The statistical model in the regression analysis suggests that low and vigorous levels of PA explain 57% of BF variance in female participants.info:eu-repo/semantics/publishedVersio

Techno economic and environmental assessment of wind assisted marine propulsion systems

In recent years, the increase in marine fuel prices coupled with stricter regulations on pollutant emissions set by the International Maritime Organization have promoted the research in new propulsion technologies and the utilisation of cleaner fuels. This paper describes a novel methodology to enable quantifying and evaluating the environmental and economic benefits that new technologies and fuels could allow in the marine sector. The proposed techno economic and environmental analysis approach enables consistent assessment of different traditional propulsion systems (diesel engine and gas turbine) when operated in conjunction with a novel environmental friendly technology, such as a vertical axis wind turbine. The techno-economic and environmental assessment is focused on the potential reduction in fuel consumption and pollutant emissions that may be accrued while operating on typical Sea Lines Of Communication (Mediterranean, North Sea, Atlantic). The study demonstrates the benefits of the installation of two vertical axis wind turbines on the deck of a ship in conjunction with conventional power plants. The analysis indicates that the performance of the wind turbines and the corresponding benefits strongly depend on the routes and environment in which they operate (therefore favourable wind conditions) allowing fuel savings from 14% (in the gas turbine case) to 16% (in the diesel engine case). The study also indicates that possible benefits may diminish for weak wind conditions. The results reported in this paper establish the economic benefits of installing vertical axis wind turbines in conjunction with conventional technology (Diesel and Gas Turbine Power plants) when installed on a ship travelling through the Atlantic Ocean. The primary purpose of this study is to introduce a methodology to demonstrate the application, performance and economic benefits of the technology at a preliminary design phase and further form a foundation for more elaborate analysis on the subject in the future

Weekly physical activity patterns of university students : are athletes more active than non‑athletes?

The aim of the present study was to compare weekly physical activity (PA) and obesity-related markers in athlete and non-athlete university students. One hundred and twenty-six university students (53 males, 20.46 ± 2.04 years old, and 73 females, 19.69 ± 1.32 years old) participated in this study. Participants were fitted with a tri-axial accelerometer (ActiGraph wGT3X-BT, Shalimar, FL, USA) to assess the daily PA. Anthropometric measures of height, weight, BMI and %fat mass were determined with a stadiometer and an electronic scale. The comparison indicated that male and female athletes had a significant lower percentage of body fat than did non-athletes (p value = 0.001; ES = 0.043). Athletes spent significantly more time in light PA than did non-athletes (p value = 0.003; ES = 0.024). Female athletes spent significantly less time in sedentary mode than did non-athletes (p value = 0.040; ES = 0.008). On the other hand, female athletes spent significantly more time in light PA (p value = 0.003; ES = 0.017) and vigorous PA (p value = 0.001; ES = 0.086) than did non-athletes. Despite some statistical differences with minimal effect size, the results of this study suggested proximity between PA levels of athletes and non-athletes, mainly in the case of sedentary behaviour. No significant effects were found in the variances of PA tested in this study.info:eu-repo/semantics/publishedVersio

Influence of vitamin C and vitamin E on redox signalling:implications for exercise adaptations

The exogenous antioxidants vitamin C (ascorbate) and vitamin E (α-tocopherol) often blunt favourable cell signalling responses to exercise, suggesting that redox signalling contributes to exercise adaptations. Current theories posit that this antioxidant paradigm interferes with redox signalling by attenuating exercise-induced reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation. The well-documented in vitro antioxidant actions of ascorbate and α-tocopherol and characterisation of the type and source of the ROS/RNS produced during exercise theoretically enables identification of the redox-dependent mechanism responsible for the blunting of favourable cell signalling responses to exercise. This review aimed to apply this reasoning to determine how the aforementioned antioxidants might attenuate exercise-induced ROS/RNS production. The principal outcomes of this analysis are (1) neither antioxidant is likely to attenuate nitric oxide signalling either directly (reaction with nitric oxide) or indirectly (reaction with derivatives, e.g. peroxynitrite) (2) neither antioxidant reacts appreciably with hydrogen peroxide, a key effector of redox signalling (3) ascorbate but not α-tocopherol has the capacity to attenuate exercise-induced superoxide generation and (4) alternate mechanisms, namely pro-oxidant side reactions and/or reduction of bioactive oxidised macromolecule adducts, are unlikely to interfere with exercise-induced redox signalling. Out of all the possibilities considered, ascorbate mediated suppression of superoxide generation with attendant implications for hydrogen peroxide signalling is arguably the most cogent explanation for blunting of favourable cell signalling responses to exercise. However, this mechanism is dependent on ascorbate accumulating at sites rich in NADPH oxidases, principal contributors to contraction mediated superoxide generation, and outcompeting nitric oxide and superoxide dismutase isoforms. The major conclusions of this review are: (1) direct evidence for interference of ascorbate and α-tocopherol with exercise-induced ROS/RNS production is lacking (2) theoretical analysis reveals that both antioxidants are unlikely to have a major impact on exercise-induced redox signalling and (3) it is worth considering alternate redox-independent mechanisms

The complex structure of the Mg II {\lambda\lambda} 2795.523, 2802.698 {\AA} regions of 64 Be stars

Here is studied the presence of absorption components shifted to the violet or the red side of the main spectral line (satellite, or discrete absorption components, i.e. SACs or DACs), in Mg II resonance lines' regions in Be stars and their kinematical characteristics. Namely our objective is to check if exists a common physical structure for the atmospheric regions creating SACs or DACs of the Mg II resonance lines. In order to do this, a statistical study of the Mg II {\lambda \lambda} 2792.523, 2802.698 {\AA} lines in the spectra of 64 Be stars of all spectral subtypes and luminosity classes is performed. We found that the absorption atmospherical regions where the Mg II resonance lines originated may be formed of several independent density layers of matter which rotate with different velocities. It is attempted also to separate SACs and DACs according to low or high radial velocity. The emission lines were detected only in the earliest and latest spectral subtypes.Comment: 18 pages, 12 figures, accepted for publication in PAS

Viral evasion of innate immune defense: The case of resistance of pandemic H1N1 influenza A virus to human mannose-binding proteins

Mannose-binding lectins effectively inhibit most seasonal strains of influenza A virus and contribute to the innate host defense vs. these viruses. In contrast, pandemic IAV strains are largely resistant to these lectins, likely contributing to increased spread and worse outcomes. In this paper, we evaluated the inhibition of IAV by mannose-binding lectins of human, bacterial, and fungal origin to understand and possibly increase activity vs. the pandemic IAV. A modified version of the human surfactant protein D (SP-D) neck and carbohydrate recognition domain (NCRD) with combinatorial substitutions at the 325 and 343 positions, previously shown to inhibit pandemic H3N2 IA

Human Pleural Fluid Elicits Pyruvate and Phenylalanine Metabolism in Acinetobacter baumannii to Enhance Cytotoxicity and Immune Evasion

The CCAAT box-harboring proteins represent a family of heterotrimeric transcription factors which is highly conserved in eukaryotes. In fungi, one of the particularly important homologs of this family is the Hap complex that separates the DNA-binding domain from the activation domain and imposes essential impacts on regulation of a wide range of cellular functions. So far, a comprehensive summary of this complex has been described in filamentous fungi but not in the yeast. In this review, we summarize a number of studies related to the structure and assembly mode of the Hap complex in a list of representative yeasts. Furthermore, we emphasize recent advances in understanding the regulatory functions of this complex, with a special focus on its role in regulating respiration, production of reactive oxygen species (ROS) and iron homeostasis.Fil: Nyah, Rodman. California State University; Estados UnidosFil: Martinez, Jasmine. California State University; Estados UnidosFil: Fung, Sammie. California State University; Estados UnidosFil: Nakanouchi, Jun. California State University; Estados UnidosFil: Myers, Amber L.. California State University; Estados UnidosFil: Harris, Caitlin M.. California State University; Estados UnidosFil: Dang, Emily. California State University; Estados UnidosFil: Fernandez, Jennifer. California State University; Estados UnidosFil: Liu, Christine. California State University; Estados UnidosFil: Mendoza, Anthony M.. California State University; Estados UnidosFil: Jimenez, Verónica. California State University; Estados UnidosFil: Nikolaidis, Nikolas. California State University; Estados UnidosFil: Brennan, Catherine A.. California State University; Estados UnidosFil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Cente; Estados Unidos. Center for Antimicrobial Resistance and Epidemiology; Estados Unidos. Case Western Reserve University School of Medicine; Estados UnidosFil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ramirez, Maria Soledad. California State University; Estados Unido

Sequence variation of koala retrovirus transmembrane protein p15E among koalas from different geographic regions

AbstractThe koala retrovirus (KoRV), which is transitioning from an exogenous to an endogenous form, has been associated with high mortality in koalas. For other retroviruses, the envelope protein p15E has been considered a candidate for vaccine development. We therefore examined proviral sequence variation of KoRV p15E in a captive Queensland and three wild southern Australian koalas. We generated 163 sequences with intact open reading frames, which grouped into 39 distinct haplotypes. Sixteen distinct haplotypes comprising 139 of the sequences (85%) coded for the same polypeptide. Among the remaining 23 haplotypes, 22 were detected only once among the sequences, and each had 1 or 2 non-synonymous differences from the majority sequence. Several analyses suggested that p15E was under purifying selection. Important epitopes and domains were highly conserved across the p15E sequences and in previously reported exogenous KoRVs. Overall, these results support the potential use of p15E for KoRV vaccine development