43 research outputs found
Water Dynamics at Protein Interfaces: Ultrafast Optical Kerr Effect Study
The behavior of water molecules surrounding a protein can have an important bearing on its structure and function. Consequently, a great deal of attention has been focused on changes in the relaxation dynamics of water when it is located at the protein surface. Here we use the ultrafast optical Kerr effect to study the H-bond structure and dynamics of aqueous solutions of proteins. Measurements are made for three proteins as a function of concentration. We find that the water dynamics in the first solvation layer of the proteins are slowed by up to a factor of 8 in comparison to those in bulk water. The most marked slowdown was observed for the most hydrophilic protein studied, bovine serum albumin, whereas the most hydrophobic protein, trypsin, had a slightly smaller effect. The terahertz Raman spectra of these protein solutions resemble those of pure water up to 5 wt % of protein, above which a new feature appears at 80 cm–1, which is assigned to a bending of the protein amide chain
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Temperature dependence of protein dynamics simulated with three different water models
The effect of variation of the water model on the temperature dependence of protein and hydration water dynamics is examined by performing molecular dynamics simulations of myoglobin with the TIP3P, TIP4P, and TIP5P water models and the CHARMM protein force field at temperatures between 20 and 300 K. The atomic mean-square displacements, solvent reorientational relaxation times, pair angular correlations between surface water molecules, and time-averaged structures of the protein are all found to be similar, and the protein dynamical transition is described almost indistinguishably for the three water potentials. The results provide evidence that for some purposes changing the water model in protein simulations without a loss of accuracy may be possible
Normes de concrétude, de valeur d'imagerie, de fréquence subjective et de valence émotionnelle pour 867 mots.
Summary : Concreteness, imageability, subjective frequency and emotionality ratings for 866 words.
This article presents concreteness, imageability, subjective and emotional valence norms for a set of 866 words. The norms were collected front 97 participants, all French native speakers whose mean age was 23. Descriptive statistics and correlational analyses have been performed on these norms and on other estimated (age of acquistion, conceptual familiarity, image variability) and objective (word frequency) published indexes. The correlational analyses reveal the composite nature of certain estimations. In particular, subjective frequency and imageability are highly correlated with the other variables. Concreteness is highly correlated only with imageability and emotional valence, the lutter which is weakly correlated with the other variables. Researchers must have at their disposal such information in order to select stimuli in factorial studies or to perform multiple regression analyses.
Key words : concreteness, imageability, subjective frequency, emotional valence.Résumé
Cet article présente des normes de concrétude, de valeur d'imagerie, de fréquence subjective et de valence émotionnelle pour 866 mots. Ces normes ont été collectées auprès de 97 participants de langue maternelle française, âgés de 23 ans en moyenne. Des statistiques descriptives et des analyses corrélationnelles ont été conduites sur ces normes et sur d'autres indices normatifs déjà publiés (âge d'acquisition, familiarité conceptuelle, variabilité d'imagerie) et objectifs (fréquence objective). Les analyses de corrélation révèlent la nature composite des estimations. En particulier, la fréquence subjective et la valeur d'imagerie sont fortement corrélées à l'ensemble des variables. La concrétude est fortement corrélée uniquement à la valeur d'imagerie et à la valence émotionnelle, laquelle est faiblement corrélée avec les autres variables. Les chercheurs doivent disposer de telles indications pour sélectionner du matériel dans le cadre d'expériences ayant recours à des plans factoriels ou pour réaliser des analyses de régression multiple.
Mots-clés: concrétude, valeur d'imagerie, fréquence subjective, valence émotionnelle.Bonin Patrick, Méot Alain, Aubert Louis-F., Malardier Nathalie, Niedenthal Paula M., Capelle-Toczek M.-C. Normes de concrétude, de valeur d'imagerie, de fréquence subjective et de valence émotionnelle pour 866 mots. In: L'année psychologique. 2003 vol. 103, n°4. pp. 655-694
Carboxyl Group Enhanced CO Tolerant GO Supported Pt Catalysts: DFT and Electrochemical Analysis
The effect of residual oxygen species
in as-prepared Pt nanoparticle
on partially reduced graphene oxide (Pt/PRGO) and partially reduced
carboxylated-GO (Pt/PRÂ(GO–COOH)) supports was investigated
using electrochemical CO stripping and density functional theory (DFT)
analysis. Pt/PRGO and Pt/PRÂ(GO–COOH) revealed a clear negative
shift in CO-stripping onset potential compared to commercial Pt/carbon
black. DFT analysis confirmed that the presence of a −COOH
group provides the most resistance for CO adsorption. This CO-Pt binding
energy is significantly lower than that observed in the presence of
an −OH group, which is the most abundant oxygen group in carbon
supports. The Pt-CO dissociation energies (on a 42-atom graphene sheet)
in the presence of various oxygen groups, in descending order, were
OH > CO ≈ C–O–C > COOH. Although
single-bonded
carbon–oxygen groups (−OH and C–O–C) are
more abundant on the GO basal plane and play an important role in
Pt nanoparticle nucleation and distribution on graphene sheets, the
double-bonded carbon–oxygen (CO and COOH) groups are
more abundant residual species post Pt nanoparticle growth and play
a vital role in enhancing CO tolerance