FOOD DISTRIBUTION RESEARCH FOR DEVELOPING COUNTRIES: DISCUSSANT

Emphasis on need for establishing practice of adoption of existing knowledge and use of problem-oriented research in developing countries.Marketing,

A Guide to Buying Canned Fruits and Vegetables

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Cycling and Health Innovative Pilot Projects (Executive summary)

The Cycling and Health Innovative Pilot Project (CHIPPS) provided cycle training for adults in Nottingham and Northamptonshire from 2007 to 2010. The Primary Care Trusts in each area have delivered these projects in collaboration with partners. In Nottingham collaboration with Ridewise delivered the Cycling for Health Project that aimed to involve people from deprived communities and employees of the Primary Care Trust; in Northamptonshire the Easy Rider project delivered via Age UK was also aimed at those living in deprived areas and middle-aged people. Throughout the three years the initiative was evaluated by the Carnegie Research Institute of Leeds Metropolitan University. Those taking part completed questionnaires at the outset, at the end of their training, three months later and finally after a year. In addition, a mix of one-to-one interviews and focus groups were conducted with policy makers, those delivering the projects and participants (including those who dropped out)

The Mayflies (Ephemeroptera) of Tennessee, With a Review of the Possibly Threatened Species Occurring Within the State

One hundred and forty-three species of mayflies are reported from the state of Tennessee. Sixteen species (Ameletus cryptostimulus, Choroterpes basalis, Baetis virile, Ephemera blanda, E. simulans, Ephemerella berneri, Heterocloeon curiosum, H. petersi, Labiobaetis ephippiatus, Leptophlebia bradleyi, Macdunnoa brunnea, Paraleptophlebia assimilis, P. debilis, P. mollis, Rhithrogenia pellucida and Siphlonurus mirus) are reported for the first time. Rare and vulnerable species occurring in the state are also discussed. This represents the first comprehensive statewide list of mayflies for Tennessee

Post-operative cranial pressure monitoring system

System for monitoring of fluidic pressures in cranial cavity uses a miniaturized pressure sensing transducer, combined with suitable amplification means, a meter with scale calibrated in terms of pressures between minus 100 and plus 900 millimeters of water, and a miniaturized chart recorder covering similar range of pressures

Grover Algorithm with zero theoretical failure rate

In standard Grover's algorithm for quantum searching, the probability of finding the marked item is not exactly 1. In this Letter we present a modified version of Grover's algorithm that searches a marked state with full successful rate. The modification is done by replacing the phase inversion by two phase rotation through angle $\phi$. The rotation angle is given analytically to be $\phi=2 \arcsin(\sin{\pi\over (4J+6)}\over \sin\beta)$, where $\sin\beta={1\over \sqrt{N}}$, $N$ the number of items in the database, and $J$ an integer equal to or greater than the integer part of $({\pi\over 2}-\beta)/(2\beta)$. Upon measurement at $(J+1)$-th iteration, the marked state is obtained with certainty.Comment: 5 pages. Accepted for publication in Physical Review

Plasma membrane association by N-acylation governs PKG function in Toxoplasma gondii

ABSTRACT Cyclic GMP (cGMP)-dependent protein kinase (protein kinase G [PKG]) is essential for microneme secretion, motility, invasion, and egress in apicomplexan parasites, However, the separate roles of two isoforms of the kinase that are expressed by some apicomplexans remain uncertain. Despite having identical regulatory and catalytic domains, PKG I is plasma membrane associated whereas PKG II is cytosolic in Toxoplasma gondii . To determine whether these isoforms are functionally distinct or redundant, we developed an auxin-inducible degron (AID) tagging system for conditional protein depletion in T. gondii . By combining AID regulation with genome editing strategies, we determined that PKG I is necessary and fully sufficient for PKG-dependent cellular processes. Conversely, PKG II is functionally insufficient and dispensable in the presence of PKG I . The difference in functionality mapped to the first 15 residues of PKG I , containing a myristoylated Gly residue at position 2 that is critical for membrane association and PKG function. Collectively, we have identified a novel requirement for cGMP signaling at the plasma membrane and developed a new system for examining essential proteins in T. gondii . IMPORTANCE Toxoplasma gondii is an obligate intracellular apicomplexan parasite and important clinical and veterinary pathogen that causes toxoplasmosis. Since apicomplexans can only propagate within host cells, efficient invasion is critically important for their life cycles. Previous studies using chemical genetics demonstrated that cyclic GMP signaling through protein kinase G (PKG)-controlled invasion by apicomplexan parasites. However, these studies did not resolve functional differences between two compartmentalized isoforms of the kinase. Here we developed a conditional protein regulation tool to interrogate PKG isoforms in T. gondii . We found that the cytosolic PKG isoform was largely insufficient and dispensable. In contrast, the plasma membrane-associated isoform was necessary and fully sufficient for PKG function. Our studies identify the plasma membrane as a key location for PKG activity and provide a broadly applicable system for examining essential proteins in T. gondii . </jats:p