22 research outputs found
A stochastic automaton shows how enzyme assemblies may contribute to metabolic efficiency
<p>Abstract</p> <p>Background</p> <p>The advantages of grouping enzymes into metabolons and into higher order structures have long been debated. To quantify these advantages, we have developed a stochastic automaton that allows experiments to be performed in a virtual bacterium with both a membrane and a cytoplasm. We have investigated the general case of transport and metabolism as inspired by the phosphoenolpyruvate:sugar phosphotransferase system (PTS) for glucose importation and by glycolysis.</p> <p>Results</p> <p>We show that PTS and glycolytic metabolons can increase production of pyruvate eightfold at low concentrations of phosphoenolpyruvate. A fourfold increase in the numbers of enzyme EI led to a 40% increase in pyruvate production, similar to that observed <it>in vivo </it>in the presence of glucose. Although little improvement resulted from the assembly of metabolons into a hyperstructure, such assembly can generate gradients of metabolites and signaling molecules.</p> <p>Conclusion</p> <p><it>in silico </it>experiments may be performed successfully using stochastic automata such as HSIM (Hyperstructure Simulator) to help answer fundamental questions in metabolism about the properties of molecular assemblies and to devise strategies to modify such assemblies for biotechnological ends.</p
Algebraic evaluation of linking numbers of unstable periodic orbits in chaotic attractors
cited By 17International audienceno abstrac
Global vector field reconstruction including a control parameter dependence
cited By 11International audienceno abstrac
Structure selection for global vector field reconstruction by using the identification of fixed points
cited By 8International audienceno abstrac
Analysis of a nonsynchronized sinusoidally driven dynamical system
cited By 7International audienceno abstrac
Analysis of a nonsynchronized sinusoidally driven dynamical system
cited By 7International audienceno abstrac
Influence of the gravity on thermal lens oscillations - Results of the 23rd ESA parabolic flight campaign
cited By 2International audienceno abstrac