Early development of the human mesonephros

The mesonephrogenic cord disintegrates into approximately 35-40 provesicular cell masses which are in close contact with the mesonephric (Wolffian) duct (WD) on their lateral side. Here, the epithelium of the WD is columnar and shares a common basal lamina with the provesicular cell masses. This in turn gives rise to a sickle-shaped pseudostratified epithelium. The concavity of the sickle is filled by spherical cells, the transition of which into the surrounding connective tissue is continuous. The sickle is transformed into a distillation flask and becomes separated from the mesonephric duct while the spherical cells maintain a connection to it by a—for the time being—solid outlet pipe. The columnar epithelium of the mesonephric duct becomes a multilayered cone, whose surface is in contact with the outlet tube. Shortly after, a continuous lumen is formed in the cone and the outlet pipe which is delimited by cells becoming columnar and forming a basal lamina. The epithelial anlage of the nephron is clearly separated from the surrounding mesenchyma by these processes. The flask eventually becomes a corpusculum, the outlet pipe a secretory (proximal) as well as collecting tubule, and the cone of the mesonephric duct a mesoureter. The various sections display differentially differentiated epithelia that are clearly distinct from each other. The mesoureter behaves differently during differentiation of epi- and paragenitale: in the epigenitale, it is short and runs into the collecting tubules of the nephrons at the lateral side of the convolved tubules, whereas a long mesoureter crosses the dorsal side of the convolved tubules and joins the corresponding collecting tubules at the far end of the mesonephros in the paragenital

Pharmacological manipulation of L-carnitine transport into L6 cells with stable overexpression of human OCTN2

Abstract.: The high-affinity Na+-dependent carnitine transporter OCTN2 (SLC22A5) has a high renal expression and reabsorbs most filtered carnitine. To gain more insight into substrate specificity of OCTN2, we overexpressed hOCTN2 in L6 cells and characterized the structural requirements of substances acting as human OCTN2 (hOCTN2) inhibitors. A 1905-bp fragment containing the hOCTN2 complete coding sequence was introduced into the pWpiresGFP vector, and L6 cells were stably transduced using a lentiviral system. The transduced L6 cells revealed increased expression of hOCTN2 on the mRNA, protein and functional levels. Structural requirements for hOCTN2 inhibition were predicted in silico and investigated in vitro. Essential structural requirements for OCTN2 inhibition include a constantly positively charged nitrogen atom and a carboxyl, nitrile or ester group connected by a 2-4-atom linker. Our cell system is suitable for studying in vitro interactions with OCTN2, which can subsequently be investigated in viv

Social evaluation at a distance – facets of stereotype content about student groups in higher distance education

In the academic domain, belonging to a negatively stereotyped group can impair performance and peer relationships. In higher distance education, stereotypes may be particularly influential as face-to-face contact is limited and non-traditional students who are at risk of being stereotyped are overrepresented. Still, research on stereotypes in higher distance education is sparse. The current research addresses this gap by investigating the Big Two of social perception (warmth, competence) and subordinate facets (friendliness, morality, assertiveness, ability, conscientiousness) in the context of higher distance education. It tests a) how well models with warmth/competence or the facets fit the data, b) whether stereotypes in higher distance education depend on the student group, and c) how the Big Two and subordinate facets predict intergroup emotions and behavioral intentions in higher distance education. An online survey with N = 626 students (74% female) of a large distance university showed that a measurement model with four facets (i.e., friendliness, morality, ability, conscientiousness) reveals adequate model fit for 12 student groups. Perceived stereotypes were positive for female students, older students, and students with children. However, migrant as well as younger students were perceived negatively. Across groups, stereotype content facets predicted intergroup emotions and behavioral intentions of facilitation or harm. Implications for the influence of negative stereotypes in higher distance education are discussed

Toll-Like Receptor 2-Deficient Mice Are Highly Susceptible to Streptococcus pneumoniae Meningitis because of Reduced Bacterial Clearing and Enhanced Inflammation

Toll-like receptor-2 (TLR2) mediates host responses to gram-positive bacterial wall components. TLR2 function was investigated in a murine Streptococcus pneumoniae meningitis model in wild-type (wt) and TLR2-deficient (TLR2−/−) mice. TLR2−/− mice showed earlier time of death than wt mice (P<.02). Plasma interleukin-6 levels and bacterial numbers in blood and peripheral organs were similar for both strains. With ceftriaxone therapy, none of the wt but 27% of the TLR2−/− mice died (P<.04). Beyond 3 hours after infection, TLR2−/− mice had higher bacterial loads in brain than did wt mice, as assessed with luciferase-tagged S. pneumoniae by means of a Xenogen-CCD (charge-coupled device) camera. After 24 h, tumor necrosis factor activity was higher in cerebrospinal fluid of TLR2−/− than wt mice (P<.05) and was related to increased blood-brain barrier permeability (Evans blue staining, P<.02). In conclusion, the lack of TLR2 was associated with earlier death from meningitis, which was not due to sepsis but to reduced brain bacterial clearing, followed by increased intrathecal inflammatio

Remote sensing of vegetation fires and its contribution to a fire management information system

In the last decade, research has proven that remote sensing can provide very useful support to fire managers. This chapter provides an overview of the type of information remote sensing can provide to the fire community. It considers first fire management information needs in the context of fire management information system. An introduction to remote sensing then precedes the description of fire information obtainable from remote sensing data (such as vegetation status, active fire detection and burned areas assessment). Finally, operational examples in five African countries illustrate how the information can be used in practice

fMRI evidence of ‘mirror’ responses to geometric shapes

Mirror neurons may be a genetic adaptation for social interaction [1]. Alternatively, the associative hypothesis [2], [3] proposes that the development of mirror neurons is driven by sensorimotor learning, and that, given suitable experience, mirror neurons will respond to any stimulus. This hypothesis was tested using fMRI adaptation to index populations of cells with mirror properties. After sensorimotor training, where geometric shapes were paired with hand actions, BOLD response was measured while human participants experienced runs of events in which shape observation alternated with action execution or observation. Adaptation from shapes to action execution, and critically, observation, occurred in ventral premotor cortex (PMv) and inferior parietal lobule (IPL). Adaptation from shapes to execution indicates that neuronal populations responding to the shapes had motor properties, while adaptation to observation demonstrates that these populations had mirror properties. These results indicate that sensorimotor training induced populations of cells with mirror properties in PMv and IPL to respond to the observation of arbitrary shapes. They suggest that the mirror system has not been shaped by evolution to respond in a mirror fashion to biological actions; instead, its development is mediated by stimulus-general processes of learning within a system adapted for visuomotor control

8. Remote Sensing Of Vegetation Fires And Its Contribution To A Fire Management Information System

In the last decade, research has proven that remote sensing can provide very useful support to fire managers. This chapter provides an overview of the types of information remote sensing can provide to the fire community. First, it considers fire management information needs in the context of a fire management information system. An introduction to remote sensing then precedes a description of fire information obtainable from remote sensing data (such as vegetation status, active fire detection and burned areas assessment). Finally, operational examples in five African countries illustrate the practical use of remotely sensed fire information. As indicated in previous chapters, fire management usually comprises activities designed to control the frequency, area, intensity or impact of fire. These activities are undertaken in different institutional, economic, social, environmental and geographical contexts, as well as at different scales, from local to national. The range of fire management activities also varies considerably according to the management issues at stake, as well as the available means and capacity to act. Whatever the level, effective fire management requires reliable information upon which to base appropriate decisions and actions. Information will be required at many different stages of this fire management system. To illustrate this, we consider a typical and generic description of a fire management loop , as provided in Figure 8.1. Fire management objectives result from fire related knowledge . For example, they may relate to sound ecological reasons for prescribed burning in a particular land management context, or to frequent, uncontrolled fires threatening valuable natural or human resources. Whatever the issues, appropriate objectives require scientific knowledge (such as fire impact on ecosystems components, such as soil and vegetation), as well as up-to date monitoring information (such as vegetation status, fire locations, land use, socioeconomic context, etc.). Policies, generally at a national and governmental level, provide the official or legal long term framework (e.g. five to ten years) to undertake actions. A proper documentation of different fire issues, and their evolution, will allow their integration into appropriate policies, whether specific to fire management, or complementary to other policies in areas such as forestry, rangeland, biodiversity, land tenure, etc. Strategies are found at all levels of fire management. They provide a shorter-term framework (e.g. one to five years) to prioritise fire management activities. They involve the development of a clear set of objectives and a clear set of activities to achieve these objectives. They may also include research and training inputs required, in order to build capacity and to answer specific questions needed to improve fire management. The chosen strategy will result from a trade-off between priority fire management objectives and the available capacity to act (e.g. institutional framework, budget, staff, etc.), and will lead towards a better allocation of resources for fire management operations to achieve specific objectives. One example in achieving an objective of conserving biotic diversity may be the implementation of a patch-mosaic burning system (Brockett et al., 200 1 ) instead of a prescribed block burning system, based on an assumption that the former should better promote biodiversity in the long-term than the latter (Parr & Brockett, 1999). This strategy requires the implementation of early season fires to reduce the size of later season fires. The knowledge of population movements, new settlements or a coming El Nino season, should help focus the resources usage, as these factors might influence the proportion as well as the locations of area burned. Another strategy may be to prioritise the grading of fire lines earlier than usual based on information on high biomass accumulation. However, whatever the strategies, they need to be based on reliable information

8. Remote Sensing Of Vegetation Fires And Its Contribution To A Fire Management Information System

In the last decade, research has proven that remote sensing can provide very useful support to fire managers. This chapter provides an overview of the types of information remote sensing can provide to the fire community. First, it considers fire management information needs in the context of a fire management information system. An introduction to remote sensing then precedes a description of fire information obtainable from remote sensing data (such as vegetation status, active fire detection and burned areas assessment). Finally, operational examples in five African countries illustrate the practical use of remotely sensed fire information. As indicated in previous chapters, fire management usually comprises activities designed to control the frequency, area, intensity or impact of fire. These activities are undertaken in different institutional, economic, social, environmental and geographical contexts, as well as at different scales, from local to national. The range of fire management activities also varies considerably according to the management issues at stake, as well as the available means and capacity to act. Whatever the level, effective fire management requires reliable information upon which to base appropriate decisions and actions. Information will be required at many different stages of this fire management system. To illustrate this, we consider a typical and generic description of a fire management loop , as provided in Figure 8.1. Fire management objectives result from fire related knowledge . For example, they may relate to sound ecological reasons for prescribed burning in a particular land management context, or to frequent, uncontrolled fires threatening valuable natural or human resources. Whatever the issues, appropriate objectives require scientific knowledge (such as fire impact on ecosystems components, such as soil and vegetation), as well as up-to date monitoring information (such as vegetation status, fire locations, land use, socioeconomic context, etc.). Policies, generally at a national and governmental level, provide the official or legal long term framework (e.g. five to ten years) to undertake actions. A proper documentation of different fire issues, and their evolution, will allow their integration into appropriate policies, whether specific to fire management, or complementary to other policies in areas such as forestry, rangeland, biodiversity, land tenure, etc. Strategies are found at all levels of fire management. They provide a shorter-term framework (e.g. one to five years) to prioritise fire management activities. They involve the development of a clear set of objectives and a clear set of activities to achieve these objectives. They may also include research and training inputs required, in order to build capacity and to answer specific questions needed to improve fire management. The chosen strategy will result from a trade-off between priority fire management objectives and the available capacity to act (e.g. institutional framework, budget, staff, etc.), and will lead towards a better allocation of resources for fire management operations to achieve specific objectives. One example in achieving an objective of conserving biotic diversity may be the implementation of a patch-mosaic burning system (Brockett et al., 200 1 ) instead of a prescribed block burning system, based on an assumption that the former should better promote biodiversity in the long-term than the latter (Parr & Brockett, 1999). This strategy requires the implementation of early season fires to reduce the size of later season fires. The knowledge of population movements, new settlements or a coming El Nino season, should help focus the resources usage, as these factors might influence the proportion as well as the locations of area burned. Another strategy may be to prioritise the grading of fire lines earlier than usual based on information on high biomass accumulation. However, whatever the strategies, they need to be based on reliable information