Metabolic responses of microbial community in soil amended with fresh leaves and leaf extracts from eucalyptus spp.

Field observations reveal that often large areas of soil surface beneath Eucalyptus occidentalis Endl. are completely bare or with scant vegetation. Moreover, previous studies have showed that other species of Eucalyptus, such as Eucalyptus camaldulensis Dehn, may be effective in suppressing seed germination and weed growth. Such effects have been ascribed to the large number of secondary metabolites within Eucalyptus leaves. Due to their inhibition activity against weeds, leaf extracts might be used for an integrated weed management context, in accordance with the Directive 2009/128/EC. Several studies exist about the effects of Eucalyptus leaf extracts on weeds, whereas they are lacking on soil microorganisms. Therefore, the aim of this study was to assess the effects of Eucalyptus leaf extracts on soil microbial biomass and activity, as well as on the relative abundance of main microbial groups. The extracts were obtained by hydrodistillation [essential oils (EOs) and hydrolates] and by water cold extraction (aqueous extracts) from leaves of both E. camaldulensis and E. occidentalis. Also, fresh leaves were tested. The soil was spread with EOs at doses of 2.64 or 3.52 \uf06dl g-1. They were applied by adding a proper water solution containing the given amount of EOs to 350 g of dry soil, so reaching 50% of its water holding capacity. The above solution held the emulsifier Fitoil at 0.5 mL L-1. Fresh leaves, dried at 40\ub0C for 48 hours, were chopped and applied at doses of 6.6 and 5.0 mg g-1 of dry soil for E. camaldulensis and E. occidentalis, respectively. Such litter application doses were those found, at field conditions (780 and 575 g m-2, respectively). Two controls were also prepared: one with water and another with water and Fitoil. After the addition of all the treatments, soils were incubated at room temperature (20-23\ub0C) in the dark for 35 days. At days 7 and 35, soils were analyzed for soil microbial biomass C and N. At the same days, the main microbial groups were investigated through the ester linked fatty acids (FAMEs). Moreover, 20 g of soil were incubated as above in 200 mL jars to determine the soil respiration rate (CO2 emission) during 36 days of incubation. Preliminary results showed, that EOs from the two species and at both doses exerted a significant biocidal action on soil microorganisms, while hydrolates, aqueous extracts and fresh leaves stimulated both microbial biomass and activity

L’acupuncture : comme alternative dans la prise en charge de la paralysie faciale peripherique idiopathique : experience tunisienne

La paralysie faciale périphérique idiopathique ou à frigoré (PfPi) est la plus fréquente des paralysies faciales. Elle pose un problème thérapeutique malgré la diversité des traitements proposés. L’acupuncture est une des alternatives proposées parmi cet arsenal thérapeutique. Le but de ce travail est d’évaluer l’apport de l’acupuncture dans le traitement de la PfPi et de dégager les différents facteurs pronostiques pouvant influencer la récupération de la fonction faciale. Notre étude a regroupé 40 patients porteurs de PfPi recrutés aux services d’ORL de l’hôpital régional de Jendouba et de l’hôpital de Charles Nicolle sur une période de 14 mois et pris en charge aux centres d’acupuncture à l’hôpital Mongi Slim et à l’hôpital régional de Jendouba. Tous les patients ont été traités, auparavant, selon le concept de la médecine occidentale, et n’ont été adressés qu’après échec du traitement médical conventionnel. L’évaluation de notre travail est basée sur deux tests : le testing musculaire de freyss et le grading de House et Brackman. L’étude statistique s’est basée sur le logiciel « SPSS ». Les résultats de cette étude montrent que le traitement par acupuncture trouve son indication lorsque le traitement médical conventionnel a échoué, même après un long délai de consultation.Mots-clés : paralysie faciale périphérique, acupuncture, traitement.The Bell’s palsy is the most common peripheral facial paralysis. it poses a therapeutic problem despite the diversity of treatments. Acupuncture is an alternative among the proposed therapeutic arsenal. The purpose of this study was to evaluate the contribution of acupuncture in the treatment of facial paralysis and to identify different prognostic factors that may influence the recovery of facial function. Our study included 40 patients with Bell’s palsy recruited in the services of ENT in Jendouba hospital and Charles Nicolle hospital over a period of 14 months and supported in centers of acupuncture in Mongi Slim hospital and Jendouba hospital. All patients were previously under the concept of medical treatment, and were sent after failure of this treatment. The evaluation of our work is based on two tests: muscle testing of freyss and grading of House and Brackman. The study is based on statistical software "SPSS". The results of this study show that treatment with acupuncture is indicated in Bell’s palsy when conventional medical treatment has failed and even after a long period of consultation.Keywords: facial palsy, acupuncture, treatment

Herbicidal activity of essential oils extracted from different Mediterranean species against Echinochloa crus galli

Weed infestation in agricultural fields can cause huge economic losses and low-quality crop yields. Echinochloa crus-galli (L.) P.Beauv. (barnyardgrass) is one of the greatest yields limiting weeds in rice cultivation systems. It is a cosmopolitan weed in both temperate and tropical regions and is reported as a weed in 36 different crops in 61 countries. The success of this weed may be attributed to the production of large numbers of small, easily dispersed seeds per plant, possession of seed dormancy, rapid development and ability to flower under a wide range of photoperiods, and relative resistance of mature plants to herbicide sprays. Thus, the best way to control barnyardgrass in an environmentally acceptable and sustainable approach is to develop eco-friendly and effective alternative means based on natural allelochemicals. Among the natural plant products, essential oils (EOs) constitute an important group. EOs may help reducing the use of synthetic herbicides and lead to less pollution and more safe agricultural products. Recently, the interest in exploring EOs with phytotoxic effects from aromatic plants for weed control has increased tremendously, because they do not persist in soil and do not leach into ground water. EOs extracted from Thymbra capitata (L.) Cav.; Mentha x piperita L.; and Santolina chamaecyparissus L. were reported to reduce the emergence and seedling growth of many weeds. The present investigation allows for a more detailed insight into the herbicidal activity of the previously mentioned EOs during spray foliar and irrigation application in post-emergence on E. crus-galli plants. T. capitata M. piperita and S. chamaecyparissus EOs were purchased from “Bordas Chinchurreta”, “Sigma-Aldrich” and and “Aromas de Ademuz”, respectively. Soil for the experiments was collected from the topsoil of a citrus field not treated with herbicides (Valencia, Spain), that was air-dried and sieved at 1 cm. E.crus-galli seeds were purchased from Herbiseed (England), and germinated in a germination-growth chamber at 30 ± 0.1 °C, 16 h in light and 20 ± 0.1 °C, 8 h in dark. Once the seedlings of E.crus-galli emerged, they were placed on pots (8x8x7 cm) filled with 2 cm of perlite at the bottom and 5 cm of the collected soil. Ten pots per treatment were prepared and placed in the greenhouse. In order to find the most effective dose of EOs and its adequate mode of application, different concentrations were used: 12, 16 and 20 µL/mL for Mentha and Santolina EOs and 4, 8 and 12 µL/mL for Thymbra EO. Fitoil was used as emulsifier at a concentration of 0.05% (v/v). Plants of barnyardgrass were treated at two-leaf stage by irrigating and spraying mode of application. To monitor the experiments, photos were taken after 24, 48 and 72 hours; and then once a week. The photos were processed with Digimizer software to obtain different data: efficacy of the treatment, level of damage, height of plants, weed plant fresh and dry weight

The factor structure of the mood disorder questionnaire in Tunisian patients

Background: The Mood Disorder Questionnaire (MDQ) is a frequently used screening tool for the early detection of Bipolar Disorder (BD), which is often unrecognized or misdiagnosed at its onset. In this study, data from Tunisia has been used to evaluate the psychometric properties of the Arabic MDQ. Methods: The sample included 151 patients with a current major depressive episode. The Arabic adapted version of the Structured Clinical Interview for DSM-IV-TR was used to formulate a diagnosis, yielding 62 patients with BD and 89 with unipolar Major Depressive Disorder (MDD). Principal component analysis with parallel analysis was used to establish the spontaneous distribution of the 13 core items of the MDQ. Confirmatory Factor Analysis (CFA) was used to check the available factor models. Receiver Operating Characteristic (ROC) analysis was used to assess the capacity of the MDQ to distinguish patients with BD from those with MDD. Results: Cronbach’s α in the sample was 0.80 (95%CI: 0.75 to 0.85). Ordinal α was 0.88. Parallel analysis suggested two main components, which explained 59% of variance in the data. CFA found a good fit for the existing unidimensional, the two-factor, and the three-factor models. ROC analysis showed that at a threshold of 7, the MDQ was able to distinguish patients with BD from those with MDD with extraordinary negative predictive value (0.92) and a positive diagnostic likelihood ratio of 3.8. Conclusion: The Arabic version of the MDQ showed good measurement properties in terms of reliability, factorial validity and discriminative properties

Alkali poisoning of Fe-Cu-ZSM-5 catalyst for the selective catalytic reduction of NO with NH3

[EN] Fe (2 wt%)-Cu (1.5 wt%)-ZSM-5 SCR catalyst contacted 1.5 wt% of Na and 1.8 wt% of K in order to simulate poisoning by species more specifically contained in exhaust gases from exhaust gases of diesel engines and power plants. Poisoning agents do not cause loss of surface area nor pore occlusion. XRD and SEM results showed that alkali metals introduction did not deteriorate the crystallinity and morphology of zeolite crystals. However, a significant loss of surface acidity was observed upon alkali-poisoned catalysts causing a dramatic deactivation of the NH3-SCR of NO reaction. Na-doped catalyst showed higher low-temperature SCR activity, while potassium has a stronger deactivation effect on Fe-Cu-ZSM-5 than sodium beyond 400 degrees C.Jouini, H.; Mejri, I.; Martinez-Ortigosa, J.; Cerrillo, JL.; Petitto, C.; Mhamdi, M.; Blasco Lanzuela, T.... (2022). Alkali poisoning of Fe-Cu-ZSM-5 catalyst for the selective catalytic reduction of NO with NH3. Research on Chemical Intermediates. 48(8):3415-3428. https://doi.org/10.1007/s11164-022-04768-93415342848

Accuracy of the Arabic HCL - 32 and MDQ in detecting patients with bipolar disorder

Background: Studies about the two most used and validated instruments for the early detection of Bipolar Disorder (BD), the 32 - item Hypomania Checklist (HCL - 32) and the Mood Disorder Questionnaire (MDQ), are scarce in non-Western countries. This study aimed to explore the reliability, factor structure, and criterion validity of their Arabic versions in a sample of Tunisian patients diagnosed with mood disorders. Methods: The sample included 59 patients with BD, 86 with unipolar Major Depressive Disorder (MDD) and 281 controls. Confirmatory factor analysis was applied to show that a single global score was an appropriate summary measure of the screeners in the sample. Receiver Operating Characteristic analysis was used to assess the capacity of the translated screeners to distinguish patients with BD from those with MDD and controls. Results: Reliability was good for both tools in all samples. The bifactor implementation of the most reported two-factor model had the best fit for both screeners. Both were able to distinguish patients diagnosed with BD from putatively healthy controls, and equally able to distinguish patients diagnosed with BD from patients with MDD. Conclusion: Both screeners work best in excluding the presence of BD in patients with MDD, which is an advantage in deciding whether or not to prescribe an antidepressant

Characterization of CTX-M ESBLs in Enterobacter cloacae, Escherichia coli and Klebsiella pneumoniae clinical isolates from Cairo, Egypt

<p>Abstract</p> <p>Background</p> <p>A high rate of resistance to 3^rdgeneration cephalosporins among Enterobacteriaceae isolates from Egypt has been previously reported. This study aims to characterize the resistance mechanism (s) to extended spectrum cephalosporins among resistant clinical isolates at a medical institute in Cairo, Egypt.</p> <p>Methods</p> <p>Nonconsecutive <it>Klebsiella pneumoniae </it>(Kp), <it>Enterobacter cloacae </it>(ENT) and <it>Escherichia coli </it>(EC) isolates were obtained from the clinical laboratory at the medical institute. Antibiotic susceptibility was tested by CLSI disk diffusion and ESBL confirmatory tests. MICs were determined using broth microdilution. Isoelectric focusing (IEF) was used to determine the pI values, inhibitor profiles, and cefotaxime (CTX) hydrolysis by the β-lactamases. PCR and sequencing were performed using <it>bla</it>_CTX-Mand IS<it>Ecp1</it>-specific primers, with DNA obtained from the clinical isolates. Conjugation experiments were done to determine the mobility of <it>bla</it>_CTX-M.</p> <p>Results</p> <p>All five clinical isolates were resistant to CTX, and were positive for ESBL screening. IEF revealed multiple β-lactamases produced by each isolate, including a β-lactamase with a pI of 8.0 in Kp and ENT and a β-lactamase with a pI of 9.0 in EC. Both β-lactamases were inhibited by clavulanic acid and hydrolyzed CTX. PCR and sequence analysis identified <it>bla</it>_CTX-M-14in Kp and ENT and a <it>bla</it>_CTX-M-15in EC. Both <it>bla</it>_CTX-M-14and <it>bla</it>_CTX-M-15were preceded by IS<it>Ecp1 </it>elements as revealed by partial sequence analysis of the upstream region of the <it>bla</it>_CTX-Mgenes. <it>bla</it>_CTX-M-15 was transferable but not <it>bla</it>_CTX-M-14.</p> <p>Conclusion</p> <p>This is the first report of CTX-M-14 in Kp and ENT isolates from Egypt, the Middle East and North Africa.</p

On the Existence of Shadow Prices

For utility maximization problems under proportional transaction costs, it has been observed that the original market with transaction costs can sometimes be replaced by a frictionless "shadow market" that yields the same optimal strategy and utility. However, the question of whether or not this indeed holds in generality has remained elusive so far. In this paper we present a counterexample which shows that shadow prices may fail to exist. On the other hand, we prove that short selling constraints are a sufficient condition to warrant their existence, even in very general multi-currency market models with possibly discontinuous bid-ask-spreads.Comment: 14 pages, 1 figure, to appear in "Finance and Stochastics

Set optimization - a rather short introduction

Recent developments in set optimization are surveyed and extended including various set relations as well as fundamental constructions of a convex analysis for set- and vector-valued functions, and duality for set optimization problems. Extensive sections with bibliographical comments summarize the state of the art. Applications to vector optimization and financial risk measures are discussed along with algorithmic approaches to set optimization problems