Accurate timekeeping is controlled by a cycling activator in Arabidopsis.

Transcriptional feedback loops are key to circadian clock function in many organisms. Current models of the Arabidopsis circadian network consist of several coupled feedback loops composed almost exclusively of transcriptional repressors. Indeed, a central regulatory mechanism is the repression of evening-phased clock genes via the binding of morning-phased Myb-like repressors to evening element (EE) promoter motifs. We now demonstrate that a related Myb-like protein, REVEILLE8 (RVE8), is a direct transcriptional activator of EE-containing clock and output genes. Loss of RVE8 and its close homologs causes a delay and reduction in levels of evening-phased clock gene transcripts and significant lengthening of clock pace. Our data suggest a substantially revised model of the circadian oscillator, with a clock-regulated activator essential both for clock progression and control of clock outputs. Further, our work suggests that the plant clock consists of a highly interconnected, complex regulatory network rather than of coupled morning and evening feedback loops. DOI:http://dx.doi.org/10.7554/eLife.00473.001

What is broken? Expected lifetime, perception of brokenness and attitude towards maintenance and repair

This paper addresses the discrepancy between the expected and actual lifetimes of vacuum cleaners considering perceived ‘brokenness’ as a driver for replacement. Among electrical products, vacuum cleaners have a high rate of domestic ownership in the UK. They also embody large quantities of greenhouse gases which could be reduced by increasing their longevity and resource efficiency (Schreiber et al., 2012). A focus on energy efficiency has only shown limited or even negative results, therefore to meet recent European Union regulations on durability requirements a focus on product longevity is needed. Around one half of new vacuum cleaner purchasers replace one less than 5 years old, below the expected lifespan, with perceived breakage, poor performance and unreliability as the major reasons for replacement. Their relative simplicity could allow vacuum cleaners to last for significantly longer. The nature of the common causes of failure is known, including stretched cords or blockages, and WRAP has developed guidelines for product improvements. However, many working or repairable machines are disposed of because they are perceived to be ‘irremediably’ broken

The joy of vacuuming? How the user experience affects vacuum cleaner longevity

An apparent reduction in the average lifetime of vacuum cleaners is explored in this paper in relation to their perceived usability and increasingly frequent product replacement. Motivations for product disposal combine perceived and real product failure with a perceived or real improved product offer. From an historical perspective, vacuum cleaners typify this pattern, continually offering a ‘cheaper and improved’ product. Vacuum cleaner manufacturers reinvigorate the sense of satisfaction and revulsion associated with extracting dirt from our homes through new performance focused product development. For example, increased motor power, filtration, bag-less machines and clear bin compartments have all acted as sales drivers, whilst cost effective materials and offshore and more efficient manufacturing have reduced purchase prices. The latter, cost-driven, processes can create machines that are more likely to be functionally and aesthetically damaged in use, reinforcing the trend for faster replacement. The market appears likely to continue to focus on improved user experience, with growth in market share for lighter weight cordless battery powered machines posing the risk of an increased environmental burden. Drawing from qualitative and quantitative research undertaken for a study for Defra, we explore the user’s relationship to the product, investigating the frustrations and joys of vacuum cleaner use and ownership. The findings illustrate that the revulsion and attraction of cleaning, as well as the tedium and satisfaction fostered by the product, have direct implications for vacuum cleaner longevity

The relationship between ideas about cleanliness and actions that affect product longevity

As Mary Douglas famously put it, ‘where there is dirt there is system’ (1991 (1966): 35). She was concerned particularly with the cultural systems that determine the ideas about dirt that motivate and constrain people’s actions with material objects. This paper assumes that such motivations and constraints may affect consumers’ willingness to keep or to dispose of their possessions, and therefore have an impact on product longevity. It reports on ongoing empirical research using product analysis, ethnographic interviews, a questionnaire and student design work into the possibility of increasing the longevity of vacuum cleaners by design interventions. Because its object of study is a cleaning product used in everyday cleaning practices, the research naturally connects with Douglas’ ideas as well as more recent work such as Dant 2003 that focuses on how people deal practically with the materiality of dirt, not determined by cultural categories. This paper builds on Vaussard et al.’s (2014) classification of individuals by their degree of concern for keeping their house clean, into ‘Spartan’, ‘Minimalistic’, ‘Caring’ and ‘Committed’ cleaners and their implications for vacuum cleaner replacement. Introducing a short history of concern about dirt since germ theory, it considers whether the desire for a more up to date/efficient/powerful/good looking/clean/shiny machine may accelerate replacement. It finally considers whether a design that ‘ages gracefully’ might have a longer life-span, either as a personal possession or as part of a service system

New paradoxical games based on Brownian ratchets

Based on Brownian ratchets, a counter-intuitive phenomenon has recently emerged -- namely, that two losing games can yield, when combined, a paradoxical tendency to win. A restriction of this phenomenon is that the rules depend on the current capital of the player. Here we present new games where all the rules depend only on the history of the game and not on the capital. This new history-dependent structure significantly increases the parameter space for which the effect operates.Comment: 4 pages, 3 eps figures, revte

Human engineering design criteria study Final report

Human engineering design criteria for use in designing earth launch vehicle systems and equipmen

Mutation of Arabidopsis SPLICEOSOMAL TIMEKEEPER LOCUS1 Causes Circadian Clock Defects

The circadian clock plays a crucial role in coordinating plant metabolic and physiological functions with predictable environmental variables, such as dusk and dawn, while also modulating responses to biotic and abiotic challenges. Much of the initial characterization of the circadian system has focused on transcriptional initiation, but it is now apparent that considerable regulation is exerted after this key regulatory step. Transcript processing, protein stability, and cofactor availability have all been reported to influence circadian rhythms in a variety of species. We used a genetic screen to identify a mutation within a putative RNA binding protein (SPLICEOSOMAL TIMEKEEPER LOCUS1 [STIPL1]) that induces a long circadian period phenotype under constant conditions. STIPL1 is a homolog of the spliceosomal proteins TFP11 (Homo sapiens) and Ntr1p (Saccharomyces cerevisiae) involved in spliceosome disassembly. Analysis of general and alternative splicing using a high-resolution RT-PCR system revealed that mutation of this protein causes less efficient splicing of most but not all of the introns analyzed. In particular, the altered accumulation of circadian-associated transcripts may contribute to the observed mutant phenotype. Interestingly, mutation of a close homolog of STIPL1, STIP-LIKE2, does not cause a circadian phenotype, which suggests divergence in function between these family members. Our work highlights the importance of posttranscriptional control within the clock mechanism. © 2012 American Society of Plant Biologists. All rights reserved

The Impact of Radiotherapy Dose on Local Control of Ewing's Sarcoma of Bone

Purpose. Improvements in the systemic management of Ewing's sarcoma of bone over the last 20 years have led to a dramatic improvement in survival. The corollary is that treatment of the primary disease requires re-evaluation, since a significant number of patients still suffer local relapse

Evaluation of low density array technology for quantitative parallel measurement of multiple genes in human tissue

BACKGROUND: Low density arrays (LDAs) have recently been introduced as a novel approach to gene expression profiling. Based on real time quantitative RT-PCR (QRT-PCR), these arrays enable a more focused and sensitive approach to the study of gene expression than gene chips, while offering higher throughput than more established approaches to QRT-PCR. We have now evaluated LDAs as a means of determining the expression of multiple genes simultaneously in human tissues and cells. RESULTS: Comparisons between LDAs reveal low variability, with correlation coefficients close to 1. By performing 2-fold and 10-fold serial dilutions of cDNA samples in the LDAs we determined a clear linear relationship between the gene expression data points over 5 orders of magnitude. We also showed that it is possible to use LDAs to accurately and quantitatively detect 2-fold changes in target copy number as well as measuring genes that are expressed with low and high copy numbers in the range of 1 × 10(2 )– 1 × 10(6 )copies. Furthermore, the data generated by the LDA from a cell based pharmacological study were comparable to data generated by conventional QRT-PCR. CONCLUSION: LDAs represent a valuable new approach for sensitive and quantitative gene expression profiling

Multiple Light Signaling Pathways Control Solar Tracking in Sunflowers

Sunflowers are famous for their ability to track the sun throughout the day and then reorient at night to face east the following morning. This occurs by differential growth patterns, with the east sides of stems growing more during the day and the west sides of stems growing more at night. This process, termed heliotropism, is generally believed to be a specialized form of phototropism; however, the underlying mechanism is unknown. To better understand heliotropism, we compared gene expression patterns in plants undergoing phototropism in a controlled environment and in plants initiating and maintaining heliotropic growth in the field. We found the expected transcriptome signatures of phototropin-mediated phototropism in sunflower stems bending towards monochromatic blue light. Surprisingly, the expression patterns of these phototropism-regulated genes are quite different in heliotropic plants. Most genes rapidly induced during phototropism display only minor differences in expression across solar tracking stems. However, some genes that are both rapidly induced during phototropism and are implicated in growth responses to foliar shade are rapidly induced on the west sides of stems at the onset of heliotropism, suggesting a possible role for red light photoreceptors in solar tracking. To test the involvement of different photoreceptor signaling pathways in heliotropism, we modulated the light environment of plants initiating solar tracking. We found that depletion of either red and far-red light or blue light did not hinder the initiation or maintenance of heliotropism in the field. Together, our results suggest that the transcriptional regulation of heliotropism is distinct from phototropin-mediated phototropism and likely involves inputs from multiple light signaling pathways