The structure of an endogenous Drosophila centromere reveals the prevalence of tandemly repeated sequences able to form i-motifs

Centromeres are the chromosomal loci at which spindle microtubules attach to mediate chromosome segregation during mitosis and meiosis. In most eukaryotes, centromeres are made up of highly repetitive DNA sequences (satellite DNA) interspersed with middle repetitive DNA sequences (transposable elements). Despite the efforts to establish complete genomic sequences of eukaryotic organisms, the so-called 'finished' genomes are not actually complete because the centromeres have not been assembled due to the intrinsic difficulties in constructing both physical maps and complete sequence assemblies of long stretches of tandemly repetitive DNA. Here we show the first molecular structure of an endogenous Drosophila centromere and the ability of the C-rich dodeca satellite strand to form dimeric i-motifs. The finding of i-motif structures in simple and complex centromeric satellite DNAs leads us to suggest that these centromeric sequences may have been selected not by their primary sequence but by their ability to form noncanonical secondary structures.Peer Reviewe

Multi-isotopic assessments of spatio-temporal diet variability: the case of two sympatric gulls in the western Mediterranean

In predator populations, changes in foraging behaviour in response to spatio-temporal variability of prey are expected. Prey depletion might cause trophic niche widening in generalist species, but not in specialists, which are expected to increase their foraging effort without diet shifts. In sympatric species feeding on similar resources, reduced food availability can increase interspecific competition and cause trophic niche segregation. To understand these processes, we studied the spatio-temporal variability in diet and niche width in 2 sympatric gull species, the yellow-legged gull Larus michahellis and Audouin’s gull Ichthyaetus audouinii, which have experienced exponential growth in recent decades due to an increase in anthropogenic food subsidies. We sampled feathers from chicks of both species in several colonies along the western Mediterranean from 2009 to 2011 and performed stable isotope analysis of carbon, nitrogen and sulphur on these feathers. Bayesian modelling shows that both species displayed opportunistic behaviour if different types of resources were available, but could also narrow their trophic niche if 1 resource was abundant. We also provide evidence of trophic segregation between the 2 gull species, suggesting the occurrence of interspecific competition for food. Our meta-population approach provides a comprehensive view of the trophic ecology and the competitive interactions of these gull species. We emphasize the usefulness of 3-dimensional isotope analyses to correctly assess spatio-temporal variability in trophic behaviour of predator species, revealing differences that would remain hidden in single population studies or when using only the isotopic ratios of 2 elements.info:eu-repo/semantics/acceptedVersio

Cytomegalovirus-Associated Pancreatitis in Acquired Immunodeficiency Syndrome

A middle-aged woman suffering from CMV pancreatitis and HIV positive was treate

Metodología analítica para la determinación de los ácidos grasos que componen el D003 mediante Cromatografía Gaseosa con columna de relleno

El D003 es una sustancia con efectos reductores de colesterol y antiagregantes plaquetarios constituida por una mezcla de ácidos grasos (AG) entre C24:0 y C36:0, la cual ha sido aislada de la cera de la caña de azúcar.1-4 El control de su calidad se realiza mediante una metodología analítica por cromatografía gaseosa (CG) con columna capilar widebore debidamente validada.5 Sin embargo, el empleo de la CG con columna de relleno puede constituir una alternativa para el control de calidad de rutina, pues pese a su menor poder de resolución respecto a la CG en columna capilar, con ella se han logrado resultados confiables y reproducibles en el control de la calidad y estudios de estabilidad de medicamentos y nutracéuticos.6,

La Basílica de Pátzcuaro en Pátzcuaro, México: su innovador sistema estructural en la fachada y cubierta

La basílica de Pátzcuaro es el único vestigio del magno proyecto catedralicio que en el S. XVI concibiera Vasco de Quiroga para la diócesis de Michoacán, es en el último tercio de este siglo que la estructura material de la ex catedral y como producto de solicitaciones sísmicas que se dan a través de tres siglos, el edificio presenta modificaciones en su estructura, presentándose las finales en el S. XIX. La vulnerabilidad sísmica obedece a una obra de fábrica de considerable luz, las intervenciones realizadas en el periodo comprendido de 1850 y 1872, básicamente en la fachada y cubierta, muestran la aplicación de los conocimientos de la época, la singularidad de la cubierta de la basílica, consiste en ser el primer edificio histórico que presenta apoyos discontinuos entre la estructura de madera y la corona del muro, interacción que le otorga un comportamiento elástico resultando en un factor de amortiguamiento ante solicitaciones sísmicas. Una singularidad lo constituye la interacción entre la estructura de madera y el mecanismo de apoyo en la corona del muro como una adecuación de la viga de Winkler.5.- Patrimonios Urbano, Rural, Industrial y Religioso. Cementerios Patrimoniales. Técnicas de Evaluación, Limpieza, Reparación y Conservación. Intervenciones en construcciones con patologías estructurales y aplicación de refuerzos

Sensitivity and representativeness of One-Health surveillance for diseases of zoonotic potential at health facilities relative to household visits in rural Guatemala

Most human and animal disease notification systems are unintegrated and passive, resulting in underreporting. Active surveillance can complement passive efforts, but because they are resource-intensive, their attributes must be evaluated. We assessed the sensitivity and representativeness of One-Health surveillance conducted at health facilities compared to health facilities plus monthly household visits in three rural communities of Guatemala. From September 2017 to November 2018, we screened humans for acute diarrheal, febrile and respiratory infectious syndromes and canines, swine, equines and bovines for syndromic events or deaths. We estimated the relative sensitivity as the incidence rate ratio of detecting an event in health facility surveillance compared to household surveillance from Poisson models. We used interaction terms between the surveillance method and sociodemographic factors or time trends to assess effect modification as a measure of relative representativeness. We used generalized additive models with smoothing splines to model incidence over time by surveillance method. We randomized 216 households to health facility surveillance and 198 to health facility surveillance plus monthly household visits. Health facility surveillance alone was less sensitive than when combined with household surveillance by 0.42 (95% CI: 0.34, 0.53), 0.56 (95% CI: 0.39, 0.79), 0.02 (95% CI: 0.00, 0.10), 0.28 (95% CI: 0.15, 0.50) and 0.22 (95% CI: 0.03, 0.92) times for human acute infections, human severe acute infections, and deaths in canines, swine and equines, respectively. Health facility surveillance alone underrepresented Spanish speakers (interaction p-value = 0.0003) and persons in higher economic assets (interaction p-values = 0.0008). The trend in incidence over time was different between the two study groups, with a larger decrease in the group with household surveillance (all interaction p-values <0.10). Surveillance at health facilities under ascertains syndromes in humans and animals which leads to underestimation of the burden of zoonotic disease. The magnitude of under ascertainment was differentially by sociodemographic factors, yielding an unrepresentative sample of health events. However, it is less time-intensive, thus might be sustained over time longer than household surveillance. The choice between methodologies should be evaluated against surveillance goals and available resources

Prueba de límite para n-hexano residual en policosanol por Espacio de Cabeza Estático -Cromatografía de Gases

Como parte del proceso de obtención de un ingrediente activo, se deben tratar de eliminar en la mayor medida posible los disolventes empleados, pues sus restos constituyen impurezas potencialmente tóxicas. Por este motivo, los organismos reguladores de la Industria Farmacéutica tienen definidos los contenidos máximos permisibles para los disolventes residuales de los ingredientes activos utilizados en la producción de medicamentos, entre los cuales el n-hexano, empleado en la obtención del policosanol, presenta un límite de 290 ppm.