Effect of feed evaluation system on the cost of rations for dairy cows

El objetivo del presente trabajo fue comparar la valoración de las necesidades y los aportes de energía calculados según sistemas históricos y modernos de alimentación para vacas lecheras -unidades alimenticias escandinavas (UA), energía neta grasa (ROSTOCK), energía metabolizable (MAFF), energía neta de lactación (NRC) y unidades forrajeras leche (INRA)- con el fin de investigar la repercusión de aquella en el coste de la ración diaria. Las necesidades energéticas se expresaron como energía neta de lactación (ENL) y se calcularon a intervalos de 30 días en leche (DEL) para una vaca Holstein adulta de 680 kg de peso vivo con una producción de 10000 kg de leche en 305 días y un intervalo entre partos de un año. El aporte de ENL según cada sistema se calculó a partir de diez raciones optimizadas con el programa CPMDairy 1.0 para satisfacer las necesidades nutritivas de la vaca modelo cada 30 DEL. Exceptuando los valores obtenidos con el sistema de unidades alimenticias y el comienzo inmediato de la lactación, las diferencias entre las necesidades de ENL calculadas según los distintos sistemas fueron relativamente bajas (entre 1,1 y 7,5%) y achacables fundamentalmente a la valoración energética del cambio de peso. El aporte calculado de energía mostró diferencias importantes entre los sistemas, oscilando de 19,1 a 25,2%. A lo largo de la lactación, los aportes calculados fueron inferiores a las necesidades en todos los sistemas, con la excepción de NRC y MAFF a 30 y 270 DEL, respectivamente, y UA desde 90 DEL. La diferencia promedio estuvo comprendida entre -1,4 ± 1,1 Mcal d-1 (4,5 ± 2,9%; MAFF) y -5,5 ± 1,7 Mcal d-1 (15,6 ± 4,5%; INRA). El coste unitario de la Mcal varió notablemente entre sistemas (de 18,7 a 25,5%) al igual que el coste de satisfacer los requerimientos diarios de energía (de 13,5 a 50,2%). Se concluyó que, independientemente de cual sea la precisión real, el sistema de valoración de energía empleado en la formulación tiene una importante repercusión sobre el coste de las raciones para vacas lecherasThe objective of the present work was to compare energy requirements and supply calculated according to old and modern feed evaluation systems for dairy cattle - Scandinavian feed units (UA), net energy fat (ROSTOCK), metabolizable energy (MAFF), net energy of lactation (NRC) and milk fodder units (INRA) - to assess the differences in the cost of the ration. The energy requirements were expressed as net energy of lactation (NEL) and were calculated every 30 days in milk (DIM) for a mature Holstein cow weighing 680 kg, producing 10000 kg of milk in 305 days, and calving every year. Ten rations optimized with CPM-Dairy 1.0 to satisfy the nutritional requirements of the model cow every 30 DIM were used to compare the calculated NEL supply according to the systems being evaluated. Except for the values obtained according to the feed units system and the onset of lactation, the differences between the systems in the calculated energy requirements were relatively low (from 1.1% to 7.5%) and mainly attributable to the energy value assigned to the live weight change. The differences between the systems in the NEL supply of the rations were high and oscillated between 19.1% and 25.2%. The calculated supplies were smaller than the requirements in all the systems throughout lactation, except for UA from 90 DIM and NRC and MAFF at 30 and 270 DIM, respectively. The average differences were in the range from -1.4 ± 1.1 Mcal d-1 (4.5 ± 2.9%; MAFF) to -5.5 ± 1.7 Mcal d-1 (15.6 ± 4.5%; INRA). The cost of the Mcal differed between the systems (from 18.7% to 25.5%) and the same was true for the cost of satisfying the daily energy requirements (from 13.5% to 50.2%). It was concluded that, without taking into account the real accuracy, the energy evaluation system used in the formulation affects considerably the cost of rations for lactating dairy cattle

Adding plant oils to dairy goat diets: Effect on fatty acid content of milk fat

[ES]: El objetivo del presente trabajo fue investigar el efecto de la adición de aceites vegetales de diferente grado de insaturación a la dieta de cabras lecheras sobre el perfil de ácidos grasos de la grasa láctea. Doce cabras de raza Malagueña fueron asignadas al azar a uno de cuatro tratamientos: dieta basal sin aceite añadido y la misma dieta basal adicionada con 48 g/d de aceite de girasol alto oleico, aceite de girasol normal o aceite de lino. La dieta estuvo compuesta por heno de alfalfa (30%) y un concentrado granulado (70%) en el que se incluyó el aceite correspondiente. Todos los aceites redujeron el contenido de ácidos grasos saturados de cadena media de la grasa láctea (P 0,05). El aceite de girasol normal incrementó el contenido de los ácidos C18:1t10, vaccénico y ruménico y aumentó el valor de la ratio linoleico/α-linolénico (P 0,05). Los resultados obtenidos permiten concluir que la adición de aceite de lino a la dieta de cabras en lactación, en comparación con la de los aceites de girasol alto oleico y normal, modifica el perfil de ácidos grasos de la grasa láctea en un sentido más favorable desde el punto de vista de la salud humana.[EN]: The aim of this study was to investigate the effects of adding differently unsaturated vegetable oils to a dairy goat diet on fatty acid content of milk fat. Twelve Malagueña goats were randomly allocated to one of four treatments: no oil basal diet and the same basal diet supplemented with 48 g/d of high oleic sunflower oil, regular sunflower oil or linseed oil. The basal diet was made of alfalfa hay and a pelleted concentrate (30:70 w/w). The concentrate included the respective oils. Supplemented diets decreased medium chain saturated fatty acid content in milk fat (P 0.05). Regular sunflower oil increased C18:1t10, vaccenic and rumenic acid contents as well as linoleic to α-linolenic acid ratio (P 0.05). It was concluded that compared to high oleic o regular sunflower oils, adding linseed oil in dairy goat diets modifies milk fat fatty acid content more favourably from the point of view of human health.Peer Reviewe

Evaluating the cellular consequences of ACLY inhibition in primary hepatocytes and pancreatic islets

Motivation: Metabolic diseases and their pandemic proportions cause a significant loss in quality and expectancy of life in around 25% of the population. Therefore, the development of more efficient therapies should be a clinical priority. This project aims to evaluate the cellular consequences of a chronic Ac-CoA cytosolic pool reduction induced by the inhibition of ATP-citrate lyase (ACLY), core enzyme in metabolism and major source of Ac-CoA within the cytoplasm. Ac-Coa is a central molecule in many metabolic pathways involved in de novo lipogenesis, cholesterol production and protein acetylation.Methods: Most of the assays were performed on mouse primary cultured hepatocytes and islets of Langerhans. Hepatocytes were obtained by perfusing the mouse liver with collagenase and isolated by filtering and differential centrifugation. Islets of Langerhans were obtained by perfusing the mouse pancreas with collagenase and were isolated by handpicking. Cells were treated with 0, 10, 30, 50 and 100 μM of SB-204990 (SB), an ACLY inhibitor with a Ki of 1 μM. Metabolic cell activity, cell death and urea secretion were measured. Radioactive metabolite tracing was performed to assess ACLY-dependent metabolic functions. Proteins of treated hepatocytes were extracted to perform western blots.Results: In primary hepatocytes, SB neither affected metabolic activity nor urea production when concentration was <50 μM SB. However, exposure to this drug resulted in a significant increase in cell toxicity at ≥30 μM SB. We detected a dose-dependent decrease in liponeogenesis upon treatment. β-oxidation of fatty acids was affected by SB following a hormetic effect, with a rise at 10 μM and a reduction at 50 and 100 μM. The GAPDH protein expression was higher at 10 μM SB, suggesting a modulation of glycolysis/gluconeogenesis. LC3B protein expression patterns indicated autophagy was reduced at 10 μM SB and incremented at 100 μM SB. In primary islets, SB did not impair metabolic activity, while cell death, determined by ELISA, showed a reduction at 30 μM and no effects at other concentrations.Conclusions: The effects of ACLY inhibition showed that this enzyme plays a key role in the cellular homeostasis of primary hepatocytes. Fatty acids catabolism and glucose utilization relies on the presence of functional ACLY. In islets, SB revealed to be non toxic. Our results suggest that ACLY may represent a promising target for the treatment of metabolc disorders

Towards a component-based framework for developing Semantic Web applications

For those outside the research community, to develop Semantic Web applications entails real difficulty. This difficulty is due in part to the lack of usable approaches for planning Semantic Web solutions, even though Semantic Web tools have already reached industrial maturity. We propose here the Semantic Web Framework, a component-based framework for analysing rapidly the required components, the dependencies between them, and selecting existing solutions. This approach has been tested with a number of industrial partners, which justifies the effort made in this direction

Diet digestibility and production performance in dairy goats consuming plant oils

The aim of this study was to investigate the effects of differently unsaturated vegetable oils added to dairy goat diets on apparent digestibility and performance traits. Twelve Malagueña goats were randomly assigned to one of four treatments: no oil (CONTROL), 48 g/d of high oleic sunflower oil (GAO), regular sunflower oil (RSFO) or linseed oil (LIN). The basal diet was made of alfalfa hay and a pelleted concentrate, which included the respective oil and chromium as indicator. The experimental period lasted 21 days. The digestibility of dietary components, except for fat, did not differ between treatments (P > 0.05). Oil inclusion in the diet significantly (P < 0.05) increased fat digestibility. There were no significant differences (P > 0.05) in dry matter intake, milk yield and milk composition between treatments. It was concluded that moderate amounts of unsaturated plant oils can be added to dairy goat diets without negative effects on diet digestibility or performance traits

Límites nutricionales para dietas de cabras lecheras en crecimiento

El objetivo del presente trabajo fue revisar el consumo de materia seca y las necesidades nutritivas de cabras lecheras de reposición estabuladas para establecer límites nutricionales aplicables a la formulación de dietas por programación lineal a mínimo coste. El diseño de dietas nutricionalmente adecuadas y económicas requiere que el nutricionista estime correctamente el consumo de materia seca y forraje y los aportes y las necesidades de nutrientes. La bibliografía revisada sugiere que el consumo total de materia seca de las cabras oscila entre los límites físico y fisiológico impuestos por la capacidad del tracto digestivo y las necesidades energéticas diarias. El efecto de llenado digestivo puede relacionarse con los carbohidratos estructurales de la dieta, mientras la saciedad metabólica se relaciona con la concentración energética de la dieta. El consumo mínimo de forraje necesario para mantener la salud ruminal es bajo, mientras que el consumo máximo de forraje está determinado probablemente por el contenido de fibra neutrodetergente de la dieta. Los aportes y las necesidades de energía y proteína calculadas según los dos sistemas de valoración más modernos son similares, por lo que es indistinto usar cualquiera de ambos para optimizar dietas. Las propuestas recientes para el cálculo de los aportes y las necesidades de calcio y fósforo ofrecen una mayor precisión

Lipid metabolism in ruminants

El objetivo de este trabajo fue revisar algunos aspectos del metabolismo lipídico y características relevantes de la grasa de la carne y la leche de los rumiantes. Los ácidos grasos disponibles para la absorción en el intestino delgado de los rumiantes proceden de los alimentos y los microorganismos ruminales, y son mayoritariamente ácidos grasos saturados y no esterificados debido a la digestión microbiana ruminal. Los ácidos grasos absorbidos que tienen menos de 12 carbonos son vertidos directamente a la vena porta y transportados al hígado unidos a la albúmina sérica; el resto son esterificados e incorporados a lipoproteínas de muy baja densidad y quilomicrones que se transportan por vía linfática hasta el torrente sanguíneo para su distribución a los tejidos. El hígado de los rumiantes tiene menor importancia en el metabolismo lipídico que el de los monogástricos, pero adquiere especial relevancia en situaciones de balance energético negativo en las que la alteración del metabolismo hepático de los lípidos puede provocar graves patologías. Los depósitos grasos distintos de la musculatura están constituidos casi exclusivamente por triglicéridos y son la principal reserva de energía del organismo. Por el contrario, la grasa intramuscular posee distintas proporciones de fosfolípidos y triglicéridos en función del grado de engrasamiento. Los fosfolípidos de las membranas celulares son el lugar preferente de deposición de los ácidos grasos poliinsaturados disponibles. La composición de la grasa láctea varía en función del origen de los ácidos grasos: ácidos grasos de cadena larga de origen alimentario o movilizados desde el tejido adiposo, o ácidos grasos de cadena corta y media sintetizados in situ a partir de acetato y betahidroxibutirato. La mayor parte de los ácidos grasos incorporados a los triglicéridos lácteos son captados de la sangre. La importante contribución de los ácidos grasos de la dieta consumida por los rumiantes a los lípidos de sus productos ofrece la posibilidad de modificar el contenido de los ácidos grasos de la carne y, sobre todo, la leche en un sentido favorable para la salud de los consumidores.In this paper, key aspects of lipid metabolism and characteristics of ruminants’ meat and milk fat were reviewed. Fatty acids available for absorption in the small intestine of ruminants are from dietary and microbial origin and, because of microbial digestion in the rumen, are mainly nonsterified saturated fatty acids. Short chain fatty acids (less than 12 carbon atoms) are absorbed into the bloodstream, bound to serum albumin and transported to the liver through the portal vein. Medium and long chain fatty acids are esterified upon their absorption and transported via lymph to the bloodstream as chylomicrons and very low density lipoproteins, to be used by the different tissues. In ruminants, the liver has a minor role in lipid metabolism compared with monogastrics, but it is especially relevant in situations of intense negative energy balance when the hepatic metabolism of lipids may be altered causing severe pathologies. Fat depots other than intramuscular depots are composed mainly of triglycerides and are the major energy reserve of the body. However, the proportion of phospholipids and triglycerides in the intramuscular fat depends on the degree of fatness. The cell membrane phospholipids are the preferred site of deposition of available polyunsaturated fatty acids. Milk fat composition depends on the origin of the fatty acids: long chain fatty acids from dietary origin or mobilized from adipose tissue, or medium and short chain fatty acids synthesized in situ from acetate and betahydroxybutyrate. Most fatty acids incorporated into milk triglycerides are taken from the blood. The major contribution of dietary fatty acids to meat and, specially, milk fatty acids offers the possibility of changing the fatty acid profile of ruminant products to promote human consumer health