Genomic and biological characterization of a velogenic Newcastle disease virus isolated from a healthy backyard poultry flock in 2010

<p>Abstract</p> <p>Background</p> <p>Newcastle disease virus (NDV) causes severe and economically important disease in poultry around the globe. None of NDV strains in Pakistan have been completely characterized and the role of rural poultry in harbouring NDV is unclear. Since they have a very important role for long-term circulation of the virus, samples were collected from apparently healthy backyard poultry (BYP) flocks. These samples were biologically analyzed using mean death time (MDT) and intracerebral pathogenicity index (ICPI), whereas genotypically characterized by the real-time PCRs coupled with sequencing of the complete genome.</p> <p>Findings</p> <p>Despite of being non-pathogenic for BYP, the isolate exhibited MDT of 49.6 h in embryonated chicken eggs and an ICPI value of 1.5. The F gene based real-time PCR was positive, whereas M-gene based was negative due to substantial changes in the probe-binding site. The entire genome of the isolate was found to be 15192 nucleotides long and encodes for six genes with an order of 3'-NP-P-M-F-HN-L-5'. The F protein cleavage site, an indicative of pathogenicity, was ¹¹²RRQKRF¹¹⁷. Complete genome comparison indicated that the RNA dependent RNA polymerase gene was the most and the phosphoprotein was least conserved gene, among all the genes. The isolate showed an Y526Q substitution in the HN protein, which determines neuraminidase receptor binding and fusion activity of NDV. Phylogenetic analysis, based on F and HN genes, classified this isolate into genotype VII, a predominant genotype responsible for ND outbreaks in Asian countries. However, it clustered well apart from other isolates in this genotype to be considered a new subgenotype (VII-f).</p> <p>Conclusions</p> <p>These results revealed that this isolate was similar to virulent strains of NDV and was avirulent in BYP either due to resistance of local breeds or due to other factors such as substantial mutations in the HN protein. Furthermore, we have characterized the first isolate of NDV, which could act as domestic reference strain and could help in development and selection of appropriate strain of NDV for vaccine in the country.</p

Związek między zmianami demograficznymi osób w wieku 45-64 lata a ich zatrudnieniem w krajach grupy Wyszehradzkiej (V4)

The aim of this research was to prove that in ageing society the increasing number of the seniors has significant role in employment. The study included Visegrad 4 countries (Czech Republic, Hungary, Poland and Slovakia). The determination and correlation coefficients and the trend equations show the different but significant connection between the employment of senior workers and their increasing number in society. If the state does not take any proactive measure to invest into the value of the human capital of older workers, the ageing, sick, unemployment citizens will cause a serious social and financing problem in the near future.Celem badań było wykazanie, że w starzejącym się społeczeństwie wzrastająca liczba seniorów odgrywa znacząca rolę w zatrudnieniu. Badaniami objęto 4 kraje należące do Grupy Wyszehradzkiej (V4), tj. Polskę, Węgry, Czechy i Słowację. Obliczone współczynniki korelacji i determinacji oraz równania trendu wskazują na różne w poszczególnych krajach, ale istotne zależności pomiędzy zatrudnieniem starszych pracowników a ich rosnącą liczbą w społeczeństwie. Jeśli kraje nie zainwestują w rozwój kapitału ludzkiego wśród osób starszych, to starzejące się społeczeństwo, osoby chore i bezrobotne mogą stać się przyczyną poważnych problemów społecznych i finansowych w najbliższej przyszłości