Characterisation of invasive group B streptococci based on investigation of surface proteins and genes encoding surface proteins

ABSTRACTThe joint distributions of the six genes bca, bac, ɛ/alp1, alp2, alp3 and rib (encoding α-C-protein, β-C-protein, ɛ/Alp1, Alp2, Alp3, and Rib, respectively) and the proteins α-C-protein, β-C-protein and Rib were investigated in invasive isolates of group B streptococcus (GBS). In total, 297 invasive isolates (123 from neonates, 174 from adults) from south-west Sweden were collected during a 13-year period. Genes were detected using multiplex and specific PCRs, and expression of the surface proteins was demonstrated using monoclonal antibodies. The genes studied were found alone or in combinations in 294 (99%) of the invasive isolates. The most common genes were rib (n = 127 isolates, 43%), alp3 (n = 78, 26%) and ɛ/alp1 (n = 42, 14%). The bac gene was never found alone, but was found in combination with one other gene in 36 isolates. The surface proteins studied were detected alone or in combinations in 152 (51%) isolates, with the most common being Rib (n = 80, 27%), α-C-protein (n = 68, 23%) and β-C-protein (n = 24, 8%). Several genes were associated significantly with particular serotypes (e.g., ɛ/alp1 with serotype Ia; bca and bac with serotypes Ib and II; rib with serotype III; alp3 with serotype V). Overall, it was concluded that demonstration of different genes and surface proteins of GBS strains can be useful in epidemiological studies and in formulation of vaccines, but disappointingly, no single gene or surface protein included in the study was sufficiently common for it to be considered as the basis for a successful GBS vaccine

Assessment of Soil Quality along the Proposed Main Road through Ngorongoro and Northern Serengeti, Tanzania

A new road through northern Serengeti National Park is proposed to be built. The purpose of this study was to collect baseline data on soils along the route of the proposed road before its construction. The physicochemical properties were used to characterize the soil before the construction of the proposed road. Levels of soil macro-elements mainly potassium (K), calcium (Ca), magnesium (Mg) and sodium (Na), and physicochemical properties: electrical conductivity (EC), pH, organic carbon (OC), soil organic matter (SOM), total nitrogen (TN), cation exchange capacity (CEC), phosphorus (P), aluminium (Al) were determined. Also, heavy metals: cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), lead (Pb), manganese (Mn), mercury (Hg), nickel (Ni) and zinc (Zn) were analyzed. This study was done on the eastern (Ngorongoro District) and western (Serengeti District) segments of the proposed road. The ranges in average values obtained for the physicochemical parameters and metal concentrations in the soils were: 0.05–0.57 mS/m for EC, 6.5–7.9 for pH, 0.71–1.23% for OC, 0.30–0.74 g/kg for TN, 1.23–2.12% for SOM, 20.6–79.7 mg/kg for available P, 0.27–2.08 meq/100-g for available K, 9.89–30.3 meq/100-g for available Ca, 0.29–0.67 meq/100-g for available Mg, 0.06–1.35 meq/100-g for available Na, 11.04–33.12 meq/100-g for CEC, 46702.9–63963.3 mg/kg for Al, BDL for Cd, 24.70–101.55 mg/kg for Cr, 27.31–34.96 mg/kg for Cu, 32390.8–42439 mg/kg for Fe, 18.06–22.19 mg/kg for Pb, 957.1–1458.9 mg/kg for Mn, BDL for Hg, 20.24–32.52 mg/kg for Ni and 96.09–124.14 mg/kg for Zn. These observed levels indicate that the soils before road construction are unpolluted, moderately fertile and within the specifications of good agricultural soil. On the western segment of the proposed road, the soil will need application of fertilizers for better agricultural usage. Keywords:    Physicochemical properties; soil quality; total nitrogen; macro-elements; heavy metals

The frequency of genes encoding three putative group B streptococcal virulence factors among invasive and colonizing isolates

BACKGROUND: Group B Streptococcus (GBS) causes severe infections in very young infants and invasive disease in pregnant women and adults with underlying medical conditions. GBS pathogenicity varies between and within serotypes, with considerable variation in genetic content between strains. Three proteins, Rib encoded by rib, and alpha and beta C proteins encoded by bca and bac, respectively, have been suggested as potential vaccine candidates for GBS. It is not known, however, whether these genes occur more frequently in invasive versus colonizing GBS strains. METHODS: We screened 162 invasive and 338 colonizing GBS strains from different collections using dot blot hybridization to assess the frequency of bca, bac and rib. All strains were defined by serotyping for capsular type, and frequency differences were tested using the Chi square test. RESULTS: Genes encoding the beta C protein (bac) and Rib (rib) occurred at similar frequencies among invasive and colonizing isolates, bac (20% vs. 23%), and rib (28% vs. 20%), while the alpha (bca) C protein was more frequently found in colonizing strains (46%) vs, invasive (29%). Invasive strains were associated with specific serotype/gene combinations. CONCLUSION: Novel virulence factors must be identified to better understand GBS disease

Changes in grassland management and linear infrastructures associated to the decline of an endangered bird population

European grassland birds are experiencing major population declines, mainly due to changes in farmland management. We analyzed the role of habitat availability, grazing management and linear infrastructures (roads and power lines) in explaining spatial and temporal variation in the population density of little bustards (Tetrax tetrax) in Portugal, during a decade in which the species population size halved. We used data from 51 areas (totaling ca. 1,50,000 ha) that were sampled in two different periods (2003–2006 and 2016). In 2003–2006, when the species occurred at high densities, habitat availability was the only factor affecting spatial variation in bustard density. In the 2016 survey, variation in density was explained by habitat availability and livestock management, with reduced bird numbers in areas with higher proportions of cattle. Population declines across the study period were steeper in areas that initially held higher densities of bustards and in areas with a higher proportion of cattle in the total stocking rate. Areas with higher densities of power lines also registered greater density declines, probably due to avoidance behavior and to increased mortality. Overall, our results show little bustards are currently lacking high quality grassland habitat, whose persistence depends on extensive grazing regimes and low linear infrastructure densitiesinfo:eu-repo/semantics/publishedVersio

Minimising Mortality in Endangered Raptors Due to Power Lines: The Importance of Spatial Aggregation to Optimize the Application of Mitigation Measures

Electrocution by power lines is one of the main causes of non-natural mortality in birds of prey. In an area in central Spain, we surveyed 6304 pylons from 333 power lines to determine electrocution rates, environmental and design factors that may influence electrocution and the efficacy of mitigation measures used to minimise electrocution cases. A total of 952 electrocuted raptors, representing 14 different species, were observed. Electrocuted raptors were concentrated in certain areas and the environmental factors associated with increased electrocution events were: greater numbers of prey animals; greater vegetation cover; and shorter distance to roads. The structural elements associated with electrocutions were shorter strings of insulators, one or more phases over the crossarm, cross-shaped design and pylon function. Of the 952 carcasses found, 148 were eagles, including golden eagle (Aquila chrysaetos), Spanish imperial eagle (Aquila adalberti) and Bonelli's eagle (Aquila fasciata). Electrocuted eagles were clustered in smaller areas than other electrocuted raptors. The factors associated with increased eagle electrocution events were: pylons function, shorter strings of insulators, higher slopes surrounding the pylon, and more numerous potential prey animals. Pylons with increased string of insulators had lower raptor electrocution rates than unimproved pylons, although this technique was unsuccessful for eagles. Pylons with cable insulation showed higher electrocution rates than unimproved pylons, both for raptors and eagles, despite this is the most widely used and recommended mitigation measure in several countries. To optimize the application of mitigation measures, our results recommend the substitution of pin-type insulators to suspended ones and elongating the strings of insulators