Self-assembly of Fmoc-tetrapeptides based on the RGDS cell adhesion motif

Self-assembly in aqueous solution has been investigated for two Fmoc [Fmoc ¼ N-(fluorenyl)-9-methoxycarbonyl] tetrapeptides comprising the RGDS cell adhesion motif from fibronectin or the scrambled sequence GRDS. The hydrophobic Fmoc unit confers amphiphilicity on the molecules, and introduces aromatic stacking interactions. Circular dichroism and FTIR spectroscopy show that the self-assembly of both peptides at low concentration is dominated by interactions among Fmoc units, although Fmoc-GRDS shows b-sheet features, at lower concentration than Fmoc-RGDS. Fibre X-ray diffraction indicates b-sheet formation by both peptides at sufficiently high concentration. Strong alignment effects are revealed by linear dichroism experiments for Fmoc-GRDS. Cryo-TEM and smallangle X-ray scattering (SAXS) reveal that both samples form fibrils with a diameter of approximately 10 nm. Both Fmoc-tetrapeptides form self-supporting hydrogels at sufficiently high concentration. Dynamic shear rheometry enabled measurements of the moduli for the Fmoc-GRDS hydrogel, however syneresis was observed for the Fmoc-RGDS hydrogel which was significantly less stable to shear. Molecular dynamics computer simulations were carried out considering parallel and antiparallel b-sheet configurations of systems containing 7 and 21 molecules of Fmoc-RGDS or Fmoc-GRDS, the results being analyzed in terms of both intermolecular structural parameters and energy contributions

Optical amplification enhancement in photonic crystals

Improving and controlling the efficiency of a gain medium is one of the most challenging problems of laser research. By measuring the gain length in an opal based photonic crystal doped with laser dye, we demonstrate that optical amplification is more than twenty-fold enhanced along the Gamma-K symmetry directions of the face centered cubic photonic crystal. These results are theoretically explained by directional variations of the density of states, providing a quantitative connection between density of the states and light amplification

Deep into the Water Fountains: The case of IRAS 18043-2116

(Abridged) The formation of large-scale (hundreds to few thousands of AU) bipolar structures in the circumstellar envelopes (CSEs) of post-Asymptotic Giant Branch (post-AGB) stars is poorly understood. The shape of these structures, traced by emission from fast molecular outflows, suggests that the dynamics at the innermost regions of these CSEs does not depend only on the energy of the radiation field of the central star. Deep into the Water Fountains is an observational project based on the results of programs carried out with three telescope facilities: The Karl G. Jansky Very Large Array (JVLA), The Australia Telescope Compact Array (ATCA), and the Very Large Telescope (SINFONI-VLT). Here we report the results of the observations towards the WF nebula IRAS 18043$-$2116: Detection of radio continuum emission in the frequency range 1.5GHz - 8.0GHz; H$_{2}$O maser spectral features and radio continuum emission detected at 22GHz, and H$_{2}$ ro-vibrational emission lines detected at the near infrared. The high-velocity H$_{2}$O maser spectral features, and the shock-excited H$_{2}$ emission detected could be produced in molecular layers which are swept up as a consequence of the propagation of a jet-driven wind. Using the derived H$_{2}$ column density, we estimated a molecular mass-loss rate of the order of $10^{-9}$M$_{\odot}$yr$^{-1}$. On the other hand, if the radio continuum flux detected is generated as a consequence of the propagation of a thermal radio jet, the mass-loss rate associated to the outflowing ionized material is of the order of 10$^{-5}$M$_{\odot}$yr$^{-1}$. The presence of a rotating disk could be a plausible explanation for the mass-loss rates estimated.Comment: 10 pages, 5 figures. Accepted for publication in A&

A Role for DPPX Modulating External TEA Sensitivity of Kv4 Channels

Shal-type (Kv4) channels are expressed in a large variety of tissues, where they contribute to transient voltage-dependent K+ currents. Kv4 are the molecular correlate of the A-type current of neurons (ISA), the fast component of ITO current in the heart, and also of the oxygen-sensitive K+ current (KO2) in rabbit carotid body (CB) chemoreceptor cells. The enormous degree of variability in the physiological properties of Kv4-mediated currents can be attributable to the complexity of their regulation together with the large number of ancillary subunits and scaffolding proteins that associate with Kv4 proteins to modify their trafficking and their kinetic properties. Among those, KChIPs and DPPX proteins have been demonstrated to be integral components of ISA and ITO currents, as their coexpression with Kv4 subunits recapitulates the kinetics of native currents. Here, we explore the presence and functional contribution of DPPX to KO2 currents in rabbit CB chemoreceptor cells by using DPPX functional knockdown with siRNA. Additionally, we investigate if the presence of DPPX endows Kv4 channels with new pharmacological properties, as we have observed anomalous tetraethylammonium (TEA) sensitivity in the native KO2 currents. DPPX association with Kv4 channels induced an increased TEA sensitivity both in heterologous expression systems and in CB chemoreceptor cells. Moreover, TEA application to Kv4-DPPX heteromultimers leads to marked kinetic effects that could be explained by an augmented closed-state inactivation. Our data suggest that DPPX proteins are integral components of KO2 currents, and that their association with Kv4 subunits modulate the pharmacological profile of the heteromultimers

Nonlinear Interaction of Transversal Modes in a CO2 Laser

We show the possibility of achieving experimentally a Takens-Bogdanov bifurcation for the nonlinear interaction of two transverse modes ($l = \pm 1$) in a $CO_2$ laser. The system has a basic O(2) symmetry which is perturbed by some symmetry-breaking effects that still preserve the $Z_2$ symmetry. The pattern dynamics near this codimension two bifurcation under such symmetries is described. This dynamics changes drastically when the laser properties are modified.Comment: 16 pages, 0 figure

Temporal and spatial variations of the absolute reflectivity of Jupiter and Saturn from 0.38 to 1.7 μ\mum with PlanetCam-UPV/EHU

We provide measurements of the absolute reflectivity of Jupiter and Saturn along their central meridians in filters covering a wide range of visible and near-infrared wavelengths (from 0.38 to 1.7 $\mu$m) that are not often presented in the literature. We also give measurements of the geometric albedo of both planets and discuss the limb-darkening behavior and temporal variability of their reflectivity values for a period of four years (2012-2016). This work is based on observations with the PlanetCam-UPV/EHU instrument at the 1.23 m and 2.2 m telescopes in Calar Alto Observatory (Spain). The instrument simultaneously observes in two channels: visible (VIS; 0.38-1.0 $\mu$m) and short-wave infrared (SWIR; 1.0--1.7 $\mu$m). We obtained high-resolution observations via the lucky-imaging method. We show that our calibration is consistent with previous independent determinations of reflectivity values of these planets and, for future reference, provide new data extended in the wavelength range and in the time. Our results have an uncertainty in absolute calibration of 10--20\%. We show that under the hypothesis of constant geometric albedo, we are able to detect absolute reflectivity changes related to planetary temporal evolution of about 5-10\%.Comment: 13 pages, 18 figures, (in press

Circulating kisspeptin and anti-müllerian hormone levels, and insulin resistance in women with polycystic ovary syndrome: a systematic review, meta-analysis, and meta-regression

Objective: This systematic review and meta-analysis aimed to summarize the available evidence regarding circulating kisspeptin and anti-müllerian hormone (AMH) and the homeostasis model assessment of insulin resistance (HOMA-IR) index in adolescents and women with and without polycystic ovary syndrome (PCOS). Method: We performed a comprehensive literature search in Medline, Embase, Cochrane, Scopus, and Web of Science for studies evaluating circulating kisspeptin levels in women with and without PCOS published until September 24th, 2020. Co-primary outcomes were the HOMA-IR index and AMH. The quality of included studies was assessed using the Newcastle-Ottawa Scale. Random-effects models were used to estimate outcomes, and effects reported as mean difference (MD) or standardized MD (SMD) and their 95 % confidence interval (CI). The systematic review and meta-analysis was registered in the International Prospective Register of Systematic Reviews (PROSPERO) as number CRD42020205030. Results: We evaluated 18 studies including, 1282 PCOS cases and 977 controls. Participants with PCOS were younger (MD = −2.38 years, 95 %CI -4.32 to -0.44), with higher BMI (MD = 1.16, 95 % CI 0.54–1.78), waist-to-hip ratio (MD = 0.04, 95 %CI 0.02 to 0.05), circulating kisspeptin (SMD = 1.15, 95 %CI 0.68–1.62), luteinizing hormone (SMD = 1.29, 95 %CI 0.76–1.83), AMH (SMD = 0.97, 95 %CI 0.60–1,34), total testosterone (SMD = 2.48, 95 %CI 1.73–3.23), free testosterone (SMD = 1.37, 95 %CI 0.56–2.17), and dehydroepiandrosterone sulphate (SMD = 0.72, 95 %CI 0.32–1.13) levels, and Ferriman-Gallwey score (SMD = 5.08, 95 %CI 2.76–7.39), and lower sex hormone-binding globulin level (SMD = −1.34, 95 %CI −2.15 to −0.52). Besides, participants with PCOS had higher HOMA-IR index (SMD = 0.76, 95 %CI 0.35–1.17), and circulating insulin (SMD = 0.75, 95 %CI 0.30–1.19), leptin (SMD = 2.82, 95 %CI 1.35–4.29), and triglycerides (SMD = 2.15, 95 %CI 1.08–3.23) levels than participants without the syndrome. The meta-regression did not identify significant factors influencing circulating kisspeptin. Conclusion: Patients with PCOS showed higher kisspeptin, LH, insulin, AMH, and androgen levels and HOMA-IR index, and lower sex hormone-binding globulin levels than those without the syndrome