41 research outputs found

    Les fondements de la distinction entre l’expropriation et la rĂ©glementation en droit international

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    Dans cet article, l’auteure propose des Ă©lĂ©ments de rĂ©ponse Ă  la question de savoir ce qui distingue l’expropriation indirecte de la rĂ©glementation en droit international. Selon elle, et contrairement Ă  ce qu’affirment certains auteurs, il n’existe pas de vide juridique sur cette question. Toutefois, un retour aux sources du droit international est nĂ©cessaire afin d’identifier des Ă©lĂ©ments de rĂ©ponse porteurs. La thĂšse principale de cet article est que la notion de propriĂ©tĂ©, combinĂ©e Ă  d’autres Ă©lĂ©ments, est dĂ©terminante dans la recherche de la ligne de dĂ©marcation entre l’expropriation et la rĂ©glementation.This article explores possible approaches to the distinction between indirect expropriation and regulation in international law. Contrary to what some authors have suggested, international law does provide solutions to this problem, although this requires a return to the fundamentals of law. This leads to a reconsideration of the concept of property, combined with other elements, as the key to differentiating between expropriation and regulation

    Characterization of Streptococcus thermophilus Host Range Phage Mutants

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    To investigate phage-host interactions in Streptococcus thermophilus, a phage-resistant derivative (SMQ-301R) was obtained by challenging a Tn917 library of phage-sensitive strain S. thermophilus SMQ-301 with virulent phage DT1. Mutants of phages DT1 and MD2 capable of infecting SMQ-301 and SMQ-301R were isolated at a frequency of 10(−6). Four host range phage mutants were analyzed further and compared to the two wild-type phages. Altogether, three genes (orf15, orf17, and orf18) contained point mutations leading to amino acid substitutions and were responsible for the expanded host range. These three proteins were also identified in both phages by N-terminal sequencing and/or matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. The results suggest that at least three phage structural proteins may be involved in phage-host interactions in S. thermophilus

    Effects of mild-overexpression of Fst-Sm/srSm type I TA system on <i>S. mutans</i> persister formation.

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    <p>Oxacillin-treated (<b>A</b>), cefotaxime-treated (<b>B</b>), and vancomycin-treated (<b>C</b>) cells were removed at the indicated time points, serially diluted, spot plated onto THYE agar plates, and the number of CFU per ml was determined from plate counts. The curves presented are the averages and standard deviations of results from three independent cultures.</p

    Bacterial strains and plasmids used in this study.

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    a<p>Em<sup>r</sup>, erythromycin resistance; Cm<sup>r</sup>, chloramphenicol resistance; Km<sup>r</sup>, kanamycin resistance.</p

    Characterization of the Fst-Sm/srSm TA system in <i>E. coli</i>.

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    <p>Cells of LMG194 containing pSK1 (Fst-Sm), pSK2 (Fst-Sm/srSm), and pSK8 (NT-Fst) were grown to mid-log phase, at which time arabinose (induced) and glucose (uninduced control) were added. After induction, appropriate dilutions were plated on LB agar for determination of the number of CFU per ml. The curves presented are the averages and standard deviations of results from three independent cultures.</p

    <i>S. mutans</i> toxicity assay based on natural competence.

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    a<p>The transformation efficiency was expressed as the percentage of chloramphenicol-resistant transformants divided by the total number of recipient cells. All experiments were performed in triplicate from three independent experiments. Statistical significance: * WT(pSK9) vs. WT(pIB166); <sup>¶</sup>ΔIGR176(pSK9) vs. ΔIGR176(pIB166).</p
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