a<p>The transformation efficiency was expressed as the percentage of chloramphenicol-resistant transformants divided by the total number of recipient cells. All experiments were performed in triplicate from three independent experiments. Statistical significance: * WT(pSK9) vs. WT(pIB166); <sup>¶</sup>ΔIGR176(pSK9) vs. ΔIGR176(pIB166).</p