Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors

ABSTRACT Colonization of plant roots by Bacillus subtilis is mutually beneficial to plants and bacteria. Plants can secrete up to 30% of their fixed carbon via root exudates, thereby feeding the bacteria, and in return the associated B. subtilis bacteria provide the plant with many growth-promoting traits. Formation of a biofilm on the root by matrix-producing B. subtilis is a well-established requirement for long-term colonization. However, we observed that cells start forming a biofilm only several hours after motile cells first settle on the plant. We also found that intact chemotaxis machinery is required for early root colonization by B. subtilis and for plant protection. Arabidopsis thaliana root exudates attract B. subtilis in vitro, an activity mediated by the two characterized chemoreceptors, McpB and McpC, as well as by the orphan receptor TlpC. Nonetheless, bacteria lacking these chemoreceptors are still able to colonize the root, suggesting that other chemoreceptors might also play a role in this process. These observations suggest that A. thaliana actively recruits B. subtilis through root-secreted molecules, and our results stress the important roles of B. subtilis chemoreceptors for efficient colonization of plants in natural environments. These results demonstrate a remarkable strategy adapted by beneficial rhizobacteria to utilize carbon-rich root exudates, which may facilitate rhizobacterial colonization and a mutualistic association with the host

Expanded encyclopaedias of DNA elements in the human and mouse genomes

All data are available on the ENCODE data portal: www.encodeproject. org. All code is available on GitHub from the links provided in the methods section. Code related to the Registry of cCREs can be found at https:// github.com/weng-lab/ENCODE-cCREs. Code related to SCREEN can be found at https://github.com/weng-lab/SCREEN.© The Author(s) 2020. The human and mouse genomes contain instructions that specify RNAs and proteins and govern the timing, magnitude, and cellular context of their production. To better delineate these elements, phase III of the Encyclopedia of DNA Elements (ENCODE) Project has expanded analysis of the cell and tissue repertoires of RNA transcription, chromatin structure and modification, DNA methylation, chromatin looping, and occupancy by transcription factors and RNA-binding proteins. Here we summarize these efforts, which have produced 5,992 new experimental datasets, including systematic determinations across mouse fetal development. All data are available through the ENCODE data portal (https://www.encodeproject.org), including phase II ENCODE1 and Roadmap Epigenomics2 data. We have developed a registry of 926,535 human and 339,815 mouse candidate cis-regulatory elements, covering 7.9 and 3.4% of their respective genomes, by integrating selected datatypes associated with gene regulation, and constructed a web-based server (SCREEN; http://screen.encodeproject.org) to provide flexible, user-defined access to this resource. Collectively, the ENCODE data and registry provide an expansive resource for the scientific community to build a better understanding of the organization and function of the human and mouse genomes.This work was supported by grants from the NIH under U01HG007019, U01HG007033, U01HG007036, U01HG007037, U41HG006992, U41HG006993, U41HG006994, U41HG006995, U41HG006996, U41HG006997, U41HG006998, U41HG006999, U41HG007000, U41HG007001, U41HG007002, U41HG007003, U54HG006991, U54HG006997, U54HG006998, U54HG007004, U54HG007005, U54HG007010 and UM1HG009442

COMPORTEMENT CRITIQUE DE DEUX FERROMAGNÉTIQUES FAIBLEMENT ANISOTROPES MISE EN ÉVIDENCE DU CROSS-OVER DÛ A L'ANISOTROPIE

Nous avons mesuré les exposants critiques β, γ et δ des sels ferromagnétiques Cu(NH4)2Br4, 2 H2O et CuRb2Br4, 2 H2O. Les lois d'échelle sont approximativement satisfaites. L'équation d'état au voisinage de Tc est proche de l'équation d'état théorique du modèle de Heisenberg à 3 dimensions. Les susceptibilités mesurées coïncident bien avec les résultats théoriques du modèle de Heisenberg. Deux cross-over dus à l'anisotropie puis aux interactions dipolaires, permettent d'expliquer les exposants critiques expérimentaux.The critical exponents β, γ, δ and the state function of ferromagnetic Cu(NH4)2Br4, 2 H2O and CuRb2Br4, 2 H2O have been determined. The experimental state function is close to the theoretical one of 3-dimensional Heisenberg model and the scaling laws are approximately fulfilled. The measured susceptibilities agree well with theoretical results for slightly anisotropic Heisenberg ferromagnet. Two cross-over, first with anisotropy, next with dipolar interactions, allow to explain the experimental values of critical exponents

Stable carbon and oxygen isotope compositions of invertebrate carbonate shells and the reconstruction of paleotemperatures and paleosalinities-A case study of the early Pleistocene of Rhodes, Greece

The coastal sediments of Rhodes in the eastern Mediterranean have recorded transgression-regression cycles that took place during the early Pleistocene. The sedimentary deposits from the Kritika Member of the Rhodes Formation consist in conglomerates, sandstones, siltstones and clays deposited in brackish to shallow marine environments. Faunal associations are dominated by molluscs and reveal rapid ecological changes. Carbon and oxygen isotope ratios of aquatic skeletal carbonates show that these ecological changes were most likely driven by large salinity changes while water temperature remained rather constant at about 22.0 ± 1.5 °C. The tectonic activity of the island rather than glacio-eustatic variations of climatic origin is advocated to be responsible for the ecological and salinity changes and sea-level variations recorded in the sedimentary sequence. © 2012 Elsevier B.V