Agrobacterium-mediated barley transformation.

More than ten years have passed since the first successful Agrobacterium-mediated barley transformation experiment, however it is still quite challenging to establish a stably functioning agroinfiltration protocol. Efficiency of the method depends mainly on the transformation and co-cultivation conditions, and also the components of the tissue-culture media. With the use of an optimized media we have been able to set up a reliable, properly functioning transformation protocol. The first generation of transgenic barley plants, transformed with a transformation cassette carrying an aldo-keto-reductase gene from Arabidopsis thaliana and the hpt marker gene, were analyzed at nucleic acid (both DNA and RNA) and at protein levels. The key factors of success proved to be the use of Silwet L-77 (surfactant) in transformation inoculum, the Cu-content of regenerating media and the continuous visual monitoring of the transformed callus during the somatic embryogenesi

Emotional Processes in Elaborating a Historical Trauma in the Daily Press

Twentieth century has witnessed several cases of mass traumatization when groups as wholes were ostracized even threated with annihilation. From the perspectives of identity trauma, when harms are afflicted to a group of people by other groups because of their categorical membership, ethnic and national traumas stand out. This paper aims to investigate long-term consequences of permanent traumatization on national identity with presenting a narrative social psychological study as a potential way of empirical exploration of the processes of collective traumatization and trauma elaboration. A Narrative Trauma Elaboration Model has been introduced which identifies linguistic markers of the elaboration process. Newspaper articles (word count = 203172) about a significant national trauma of the Hungarian history, Treaty of Trianon (1920), were chosen from a ninety year time span and emotional expressions of narratives were analysed with a narrative categorical content analytic tool (NarrCat). Longitudinal pattern of data show very weak emotional processing of the traumatic event. Results are discussed in terms of collective victimhood as core element of national identity and its effects on trauma elaboration

Wiener Index and Remoteness in Triangulations and Quadrangulations

Let $G$ be a a connected graph. The Wiener index of a connected graph is the sum of the distances between all unordered pairs of vertices. We provide asymptotic formulae for the maximum Wiener index of simple triangulations and quadrangulations with given connectivity, as the order increases, and make conjectures for the extremal triangulations and quadrangulations based on computational evidence. If $\overline{\sigma}(v)$ denotes the arithmetic mean of the distances from $v$ to all other vertices of $G$, then the remoteness of $G$ is defined as the largest value of $\overline{\sigma}(v)$ over all vertices $v$ of $G$. We give sharp upper bounds on the remoteness of simple triangulations and quadrangulations of given order and connectivity