Fine Scale Nest Site Selection of Greater Sage-Grouse In The Centennial Valley, Montana

The purpose of this study was to determine fine scale nest site selection of greater sage-grouse (Centrocercus urophasianus) in the Centennial Valley, MT. A total of ninety nests were found during 2014-2015 using radio-collared sage-grouse. Vegetation surveys were conducted at nests and random sites that measured the nest shrub and the cover available within 3m of the nest. Length of the branch over the nest (Lgth.LB), average axis width of the nest shrub (AvgAxis), lateral cover of the nest shrub (LCShrub), aerial cover of the nest shrub (ACShrub), and height of the lower branch over the nest (Ht.LB) were the habitat variables that received the most support. All habitat variables that were included in the top model were nest shrub morphological characteristics and cover provided by the nest shrub. Therefore, there is strong support that sage-grouse in the Centennial Valley are selecting nest sites based on the morphology of the nest shrub and the cover provided by that nest shrub. None of the habitat variables associated with herbaceous cover received much support for inclusion in our models. On average, residual cover (i.e. grass from previous year) provided concealment for only 4% of the nest bowl. The relative probability of a shrub being selected for a nest site is maximized when Lgth.LB >75cm long, AvgAxis >130cm wide, LCShrub >80%, and ACShrub > 70%. Managers should focus on conserving mountain big sagebrush (Artemisia tridentata ssp. vaseyana) and three-tip sagebrush (Artemisia tripartita) habitats because they were more likely to meet those shrub characteristics

Niche shifts and energetic condition of songbirds in response to phenology of food-resource availability in a high-elevation sagebrush ecosystem

Seasonal fluctuations in food availability can affect diets of consumers, which in turn may influence the physiological state of individuals and shape intra- and inter-specific patterns of resource use. High-elevation ecosystems often exhibit a pronounced seasonal “pulse” in productivity, although few studies document how resource use and energetic condition by avian consumers change in relation to food-resource availability in these ecosystems. We tested the hypothesis that seasonal increases (pulses) in food resources in high-elevation sagebrush ecosystems result in 2 changes after the pulse, relative to the before-pulse period: (1) reduced diet breadth of, and overlap between, 2 sympatric sparrow species; and (2) enhanced energetic condition in both species. We tracked breeding-season diets using stable isotopes and energetic condition using plasma metabolites of Brewer\u27s Sparrows (Spizella breweri), Vesper Sparrows (Pooecetes gramineus), and their food resources during 2011, and of only Brewer\u27s Sparrows and their food resources during 2013. We quantify diet breadth and overlap between both species, along with coincident physiological consequences of temporal changes in resource use. After invertebrate biomass increased following periods of rainfall in 2011, dietary breadth decreased by 35% in Brewer\u27s Sparrows and by 48% in Vesper Sparrows, while dietary overlap decreased by 88%. Energetic condition of both species increased when dietary overlap was lower and diet breadth decreased, after the rapid rise of food-resource availability. However, energetic condition of Brewer\u27s Sparrows remained constant in 2013, a year with low precipitation and lack of a strong pulse in food resources, even though the species\u27 dietary breadth again decreased that year. Our results indicate that diet breadth and overlap in these sparrow species inhabiting sagebrush ecosystems generally varied as predicted in relation to intra- and interannual changes in food resources, and this difference in diet was associated with improved energetic condition of sparrows at least in one year

Data from: The effects of timing of grazing on plant and arthropod communities in high-elevation grasslands

Livestock grazing can be used as a key management tool for maintaining healthy ecosystems. However, the effectiveness of using grazing to modify habitat for species of conservation concern depends on how the grazing regime is implemented. Timing of grazing is one grazing regime component that is less understood than grazing intensity and grazer identity, but is predicted to have important implications for plant and higher trophic level responses. We experimentally assessed how timing of cattle grazing affected plant and arthropod communities in high-elevation grasslands of southwest Montana to better evaluate its use as a tool for multi-trophic level management. We manipulated timing of grazing, with one grazing treatment beginning in mid-June and the other in mid-July, in two experiments conducted in different grassland habitat types (i.e., wet meadow and upland) in 2011 and 2012. In the upland grassland experiment, we found that both early and late grazing treatments reduced forb biomass, whereas graminoid biomass was only reduced with late grazing. Grazing earlier in the growing season versus later did not result in greater recovery of graminoid or forb biomass as expected. In addition, the density of the most ubiquitous grassland arthropod order (Hemiptera) was reduced by both grazing treatments in upland grasslands. A comparison of end-of-season plant responses to grazing in upland versus wet meadow grasslands revealed that grazing reduced graminoid biomass in the wet meadow and forb biomass in the upland, irrespective of timing of grazing. Both grazing treatments also reduced end-of-season total arthropod and Hemiptera densities and Hemiptera biomass in both grassland habitat types. Our results indicate that both early and late season herbivory affect many plant and arthropod characteristics in a similar manner, but grazing earlier may negatively impact species of conservation concern requiring forage earlier in the growing season

The effects of timing of grazing on plant and arthropod communities in high-elevation grasslands.

Livestock grazing can be used as a key management tool for maintaining healthy ecosystems. However, the effectiveness of using grazing to modify habitat for species of conservation concern depends on how the grazing regime is implemented. Timing of grazing is one grazing regime component that is less understood than grazing intensity and grazer identity, but is predicted to have important implications for plant and higher trophic level responses. We experimentally assessed how timing of cattle grazing affected plant and arthropod communities in high-elevation grasslands of southwest Montana to better evaluate its use as a tool for multi-trophic level management. We manipulated timing of grazing, with one grazing treatment beginning in mid-June and the other in mid-July, in two experiments conducted in different grassland habitat types (i.e., wet meadow and upland) in 2011 and 2012. In the upland grassland experiment, we found that both early and late grazing treatments reduced forb biomass, whereas graminoid biomass was only reduced with late grazing. Grazing earlier in the growing season versus later did not result in greater recovery of graminoid or forb biomass as expected. In addition, the density of the most ubiquitous grassland arthropod order (Hemiptera) was reduced by both grazing treatments in upland grasslands. A comparison of end-of-season plant responses to grazing in upland versus wet meadow grasslands revealed that grazing reduced graminoid biomass in the wet meadow and forb biomass in the upland, irrespective of timing of grazing. Both grazing treatments also reduced end-of-season total arthropod and Hemiptera densities and Hemiptera biomass in both grassland habitat types. Our results indicate that both early and late season herbivory affect many plant and arthropod characteristics in a similar manner, but grazing earlier may negatively impact species of conservation concern requiring forage earlier in the growing season

Dataset for finalized Arctic Grayling MS_Cutting et al. 2016

The excel table contains all the data used in the manuscript by Cutting et al. (2016) titled, "Seasonal Change in Trophic Niche of Adfluvial Arctic Grayling (Thymallus arcticus) and Coexisting Fishes in a High-elevation Lake System". Data are separated by individual spread sheets names. Individuals sheets can be used to reproduce all figures and analyses

Data from: Seasonal change in trophic niche of adfluvial arctic grayling (Thymallus arcticus) and coexisting fishes in a high-elevation lake system

Introduction of non-native species is a leading threat to global aquatic biodiversity. Competition between native and non-native species is often influenced by changes in food availability or suitable habitat conditions. We investigated diet breadth and degree of trophic niche overlap for a fish assemblage of native and non-native species inhabiting a shallow, high elevation lake system. This assemblage includes one of the last remaining post-glacial endemic populations of adfluvial Arctic grayling (Thymallus arcticus) in the contiguous United States. We examined gut contents and stable isotope values of fish taxa in fall and spring to assess both short- (within season) and long-term (between season) changes in trophic niches. We incorporated these data into a secondary isotopic analysis using a Bayesian statistical framework to estimate long-term trophic niche. Our data suggest that in this system, Arctic grayling share both a short- and long-term common food base with non-native trout of cutthroat x rainbow hybrid species (Oncorhynchus clarkia bouvieri x Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis). In addition, trophic niche overlap among Arctic grayling, hybrid trout, and brook trout appeared to be stronger during spring. In contrast, the native species of Arctic grayling, burbot (Lota lota), and suckers (Catostomus spp.) largely consumed different prey items. Our results suggest strong seasonal differences in trophic niche overlap among non-native trout and Arctic grayling, with a potential for greatest competition for food during spring. We suggest that conservation of endemic Arctic grayling in high-elevation lakes will require recognition of the potential for coexisting non-native taxa to impede well-intentioned recovery efforts

Use of Stable Isotopes to Investigate Keratin Deposition in the Claw Tips of Ducks

<div><p>Stable isotopes derived from the claws of birds could be used to determine the migratory origins of birds if the time periods represented in excised sections of claws were known. We investigated new keratin growth in the claws of adult female Lesser Scaup (<i>Aythya affinis</i>) by estimating the equilibration rates of stable isotopes (<i>δ</i>¹³C, <i>δ</i>¹⁵N, and <i>δ</i>²H) from the breeding grounds into 1 mm claw tips. We sampled birds on their breeding ground through time and found that it took approximately 3–3.5 months for isotope values in most claw tips to equilibrate to isotope values that reflected those present in the environment on their breeding grounds. Results from this study suggest that isotopes equilibrate slowly into claw tips of Lesser Scaup, suggesting isotopes could potentially be used to determine the wintering grounds of birds. We suggest using controlled feeding experiments or longitudinal field investigations to understand claw growth and isotopic equilibration in claw tips. Such information would be valuable in ascertaining whether claw tips can be used in future studies to identify the migratory origins of birds. </p> </div

FishMovementAccepted

Data file used for movement velocity analysis

GraylingPassageAccepted

Data file used for both grayling passage and cumulative passage analysis