Expression of CK-19 and CEA MRNA in peripheral blood of gastric cancer patients

The aim of this paper is to investigate the clinical and pathological relevance of detection of circulating tumor cells (CTC) in the peripheral blood of gastric carcinoma patients before operation

Expression of CK-19 and CEA mRNA in peripheral blood of gastric cancer patients

Aim: To investigate the clinical and pathological relevance of detection of circulating tumor cells (CTC) in the peripheral blood of gastric carcinoma patients before operation. Patients and Methods: Fifty patients with gastric adenocarcinoma were analysed prospectively. Patients were divided into two groups according to the extent of the tumor. Group I (unresectable) consisted of 22, and group II (resectable) consisted of 28 patients. Peripheral blood samples were collected pre-operatively from all 50 patients as well as from ten healthy controls and analyzed for carcinoembryonic antigen (CEA) and cytokeratin-19 (CK-19) messenger ribonucleic acids (mRNAs). Tumor localisation, stage, presence of signet cell formation, nodal metastases, serousal and lymphovascular invasion were recorded for all patients. Results: Expression of CK-19 was detected in 24 (48%), and CEA in 10 (20%) cases. Nine patients (40%) in group I and 15 (53.6%) in group II were positive for CK-19 expression. CEA expression was more frequent among group I patients (6 vs. 4 cases). There was no significant difference between the groups in the expression of CK-19 and CEA mRNA, tumor localisation, presence of signet formation, and presence and extent of nodal metastases. Patients with major vascular invasion (MVI) expressed significantly higher levels of CTC mRNA compared to those without MVI (p = 0.023 for CEA, and p = 0.009 for CK-19). The median 1 and 2-year survival was 9.5 and 10.5 months for group I, and 20 and 28.5 months for group II, respectively (p = 0.001). The mean survival was 6.7 months for patients with MVI, and 30.2 months for those without MVI (p = 0.0001). Conclusions: High levels of CTCs were observed in patients with MVI invasion, rather than other causes of unresectability. It can be suggested that expression of both CEA and CK-19 in the peripheral blood of gastric cancer patients are strong predictors of MVI and significantly worse survival rates. Copyright © Experimental Oncology, 2010

Electrodeposition of Cd,Te,CdS and CdTe on Platinum and ITO/glass electrodes. Surface characterization of CdS/ITO, CdTe/ITO and CdS/CdTe/ITO electrodes

Electrodeposition of Cd and Te has been investigated on platinum and ITO(indium tin oxide)/glass electrodes to obtain the optimum deposition conditions of CdS and CdTe on ITO/glass electrodes. Formation of CdS and CdTe thin films by cathodic deposition on different substrates (Pt, ITO) is described along with the influence of the substrate, pH, deposition potential and concentration of ions. Surface morphology and stochiometry of the CdS. CdTe and CdS/CdTe/ITO electrodes have been studied by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS)

Lymphoedema after mastectomy for breast cancer: Importance of supportive care

WOS: 000339966100003PubMed: 25216094Background. Lymphoedema resulting from axillary lymph node dissection remains a challenging complication after modified radical mastectomy. Objective. To examine the effects of supportive therapy such as rehabilitation and medical and physical treatment on the development of lymphoedema, in an attempt to establish non-surgical ways to help prevent or reduce it. Methods. Patients (N= 5 064) who underwent breast cancer surgery in the Department of General Surgery, Ankara Oncology Research and Training Hospital, Turkey, between 1995 and 2010 were included. Data were collected by retrospectively examining all the patients' files and the pre- and postoperative breast cancer follow-up forms. Results. Of the patients in the study, 19.9% developed lymphoedema. It was significantly less common in patients who participated in physiotherapy than in those who did not, and it was more common in patients with a body mass index (BMI, kg/m(2)) between 30 and 34.9 than in those with lower BMIs. Postoperative axillary radiotherapy did not affect the occurrence of lymphoedema. Conclusion. It is clear that the most successful method to reduce the impact of lymphoedema is to prevent it. We believe that educating patients about the risk factors for developing lymphoedema and referring them to postoperative physical therapy and rehabilitation clinics are the most important ways to avoid this distressing condition